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Literature DB >> 3529391

A genetic approach to analyzing membrane protein topology.

Abstract

Fusions of the secreted protein alkaline phosphatase to an integral cytoplasmic membrane protein of Escherichia coli showed different activities depending on where in the membrane protein the alkaline phosphatase was fused. Fusions to positions in or near the periplasmic domain led to high alkaline phosphatase activity, whereas those to positions in the cytoplasmic domain gave low activity. Analysis of alkaline phosphatase fusions to membrane proteins of unknown structure may thus be generally useful in determining their membrane topologies.

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Year: 1986 PMID： 3529391 DOI： 10.1126/science.3529391

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

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Cited

234 in total

1. Analysis of F factor TraD membrane topology by use of gene fusions and trypsin-sensitive insertions.

Authors: M H Lee; N Kosuk; J Bailey; B Traxler; C Manoil
Journal: J Bacteriol Date: 1999-10 Impact factor: 3.490

2. MPtopo: A database of membrane protein topology.

Authors: S Jayasinghe; K Hristova; S H White
Journal: Protein Sci Date: 2001-02 Impact factor: 6.725

3. Genome scanning in Haemophilus influenzae for identification of essential genes.

Authors: K A Reich; L Chovan; P Hessler
Journal: J Bacteriol Date: 1999-08 Impact factor: 3.490

4. The structure of multiple polypeptide domains determines the signal recognition particle targeting requirement of Escherichia coli inner membrane proteins.

Authors: J A Newitt; N D Ulbrandt; H D Bernstein
Journal: J Bacteriol Date: 1999-08 Impact factor: 3.490

5. Mutational analysis and membrane topology of ComP, a quorum-sensing histidine kinase of Bacillus subtilis controlling competence development.

Authors: F Piazza; P Tortosa; D Dubnau
Journal: J Bacteriol Date: 1999-08 Impact factor: 3.490

A genetic approach to analyzing membrane protein topology.

1. Analysis of F factor TraD membrane topology by use of gene fusions and trypsin-sensitive insertions.

2. MPtopo: A database of membrane protein topology.

3. Genome scanning in Haemophilus influenzae for identification of essential genes.

4. The structure of multiple polypeptide domains determines the signal recognition particle targeting requirement of Escherichia coli inner membrane proteins.

5. Mutational analysis and membrane topology of ComP, a quorum-sensing histidine kinase of Bacillus subtilis controlling competence development.

6. Divergent evolution of membrane protein topology: the Escherichia coli RnfA and RnfE homologues.

Review 7. Membrane topology and insertion of membrane proteins: search for topogenic signals.

8. Green fluorescent protein functions as a reporter for protein localization in Escherichia coli.

9. The ArcB sensor kinase of Escherichia coli: genetic exploration of the transmembrane region.

10. Membrane topology of the Bacillus subtilis pro-sigma(K) processing complex.