Literature DB >> 7908004

Genome size and restriction fragment length polymorphism analysis of Vibrio cholerae strains belonging to different serovars and biotypes.

S R Choudhury1, R K Bhadra, J Das.   

Abstract

The genome size of Vibrio cholerae has been determined by pulsed field gel electrophoresis following digestion of chromosomal DNA with endonucleases. The genome size of all the classical strains examined was about 3000 kb and that of El Tor biotype was 2500 kb. The NotI and SfiI digestion patterns of the genomes of several V. cholerae strains belonging to different serovars and biotypes showed distinct restriction fragment length polymorphism (RFLP). RFLP analysis together with the genome size can be used to differentiate strains of different serovars and biotypes of V. cholerae.

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Year:  1994        PMID: 7908004     DOI: 10.1111/j.1574-6968.1994.tb06659.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN: 0378-1097            Impact factor:   2.742


  10 in total

1.  Resurgent Vibrio cholerae O139: rearrangement of cholera toxin genetic elements and amplification of rrn operon.

Authors:  G Khetawat; R K Bhadra; S Nandi; J Das
Journal:  Infect Immun       Date:  1999-01       Impact factor: 3.441

2.  Vibrio cholerae O139 Bengal: combined physical and genetic map and comparative analysis with the genome of V. cholerae O1.

Authors:  G Khetawat; R K Bhadra; S Kar; J Das
Journal:  J Bacteriol       Date:  1998-09       Impact factor: 3.490

3.  Phenotypic and genotypic biotyping of environmental strains of Vibrio cholerae non-O1 isolated in Italy.

Authors:  E Filetici; L Bonadonna; M Ciccozzi; M P Anastasio; M Fantasia; T Shimada
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

4.  Genetic diversity of clinical and environmental isolates of Vibrio cholerae determined by amplified fragment length polymorphism fingerprinting.

Authors:  S C Jiang; M Matte; G Matte; A Huq; R R Colwell
Journal:  Appl Environ Microbiol       Date:  2000-01       Impact factor: 4.792

5.  Genetic diversity of Vibrio cholerae in Chesapeake Bay determined by amplified fragment length polymorphism fingerprinting.

Authors:  S C Jiang; V Louis; N Choopun; A Sharma; A Huq; R R Colwell
Journal:  Appl Environ Microbiol       Date:  2000-01       Impact factor: 4.792

Review 6.  Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing.

Authors:  F C Tenover; R D Arbeit; R V Goering; P A Mickelsen; B E Murray; D H Persing; B Swaminathan
Journal:  J Clin Microbiol       Date:  1995-09       Impact factor: 5.948

7.  Ordered cloned DNA map of the genome of Vibrio cholerae 569B and localization of genetic markers.

Authors:  S Chatterjee; A K Mondal; N A Begum; S Roychoudhury; J Das
Journal:  J Bacteriol       Date:  1998-02       Impact factor: 3.490

8.  Molecular epidemiology of Vibrio cholerae O139 in China: polymorphism of ribotypes and CTX elements.

Authors:  Mei Qu; Jing Xu; Yanpeng Ding; Ruibai Wang; Peng Liu; Biao Kan; Guoming Qi; Yanqing Liu; Shouyi Gao
Journal:  J Clin Microbiol       Date:  2003-06       Impact factor: 5.948

9.  Physical map of the genome of Vibrio cholerae 569B and localization of genetic markers.

Authors:  R Majumder; S Sengupta; G Khetawat; R K Bhadra; S Roychoudhury; J Das
Journal:  J Bacteriol       Date:  1996-02       Impact factor: 3.490

10.  Enterobacterial repetitive intergenic consensus sequences and the PCR to generate fingerprints of genomic DNAs from Vibrio cholerae O1, O139, and non-O1 strains.

Authors:  I G Rivera; M A Chowdhury; A Huq; D Jacobs; M T Martins; R R Colwell
Journal:  Appl Environ Microbiol       Date:  1995-08       Impact factor: 4.792

  10 in total

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