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Literature DB >> 7906570

Rapid mapping of cosmid clones on pig chromosomes by fluorescence in situ hybridization.

M Yerle¹, A Goureau, J Gellin, P Le Tissier, C Moran.

Abstract

Nineteen cosmids have been mapped to pig chromosomes by fluorescence in situ hybridization. Two kinds of cosmid clones were isolated as potential physical and genetic markers for the pig genome. Anonymous cosmids were obtained by screening a commercial cosmid library and were localized to Chromosomes (Chrs) 1, 2, 6, 7, 8, 10, 11, 12, 13, and 14. Some of these cosmids were found to reveal RFLP type DNA polymorphism. Microsatellite-containing cosmid clones were isolated by screening a pig cosmid library with a (CA)10 probe and were regionally mapped to Chrs 2, 6, 7, 13, and 14. Ten of the 19 chromosomes in the pig were labeled with these probes. Two-color fluorescence in situ hybridization was used to increase the efficiency of the cosmid localizations.

Entities: Chemical

Mesh：

Year: 1994 PMID： 7906570 DOI： 10.1007/BF00360565

Source DB: PubMed Journal: Mamm Genome ISSN： 0938-8990 Impact factor: 2.957

9 in total

1. Assignment of the porcine tumour necrosis factor alpha and beta genes to the chromosome region 7p11-q11 by in situ hybridization.

Authors: S Solinas; U Pauli; P Kuhnert; E Peterhans; R Fries
Journal: Anim Genet Date: 1992 Impact factor: 3.169

2. The swine steroid 21-hydroxylase gene (CYP21): cloning and mapping within the swine leucocyte antigen complex.

Authors: C Geffrotin; P Chardon; D F de Andres-Cara; R Feil; C Renard; M Vaiman
Journal: Anim Genet Date: 1990 Impact factor: 3.169

3. Rapid subchromosomal localization of cosmids by nonradioactive in situ hybridization.

Authors: T Kievits; J G Dauwerse; J Wiegant; P Devilee; M H Breuning; C J Cornelisse; G J van Ommen; P L Pearson
Journal: Cytogenet Cell Genet Date: 1990

4. A cosmid vector for systematic chromosome walking.

Authors: S H Cross; P F Little
Journal: Gene Date: 1986 Impact factor: 3.688

5. Pig microsatellites isolated from cosmids revealing polymorphism and localized on chromosomes.

Authors: A Robic; J L Parrou; M Yerle; A Goureau; M Dalens; D Milan; J Gellin
Journal: Anim Genet Date: 1995-02 Impact factor: 3.169

6. Localization of the porcine growth hormone gene to chromosome 12p1.2-->p1.5.

Authors: M Yerle; Y Lahbib-Mansais; P D Thomsen; J Gellin
Journal: Anim Genet Date: 1993-04 Impact factor: 3.169

7. Mapping of human chromosome Xq28 by two-color fluorescence in situ hybridization of DNA sequences to interphase cell nuclei.

Authors: B J Trask; H Massa; S Kenwrick; J Gitschier
Journal: Am J Hum Genet Date: 1991-01 Impact factor: 11.025

8. Rapid physical mapping of cloned DNA on banded mouse chromosomes by fluorescence in situ hybridization.

Authors: A L Boyle; D M Feltquite; N C Dracopoli; D E Housman; D C Ward
Journal: Genomics Date: 1992-01 Impact factor: 5.736

9. Localization of the pig luteinizing hormone/choriogonadotropin receptor gene (LHCGR) by radioactive and nonradioactive in situ hybridization.

Authors: M Yerle; O Galman; Y Lahbib-Mansais; J Gellin
Journal: Cytogenet Cell Genet Date: 1992

9 in total

16 in total

Rapid mapping of cosmid clones on pig chromosomes by fluorescence in situ hybridization.

1. Assignment of the porcine tumour necrosis factor alpha and beta genes to the chromosome region 7p11-q11 by in situ hybridization.

2. The swine steroid 21-hydroxylase gene (CYP21): cloning and mapping within the swine leucocyte antigen complex.

3. Rapid subchromosomal localization of cosmids by nonradioactive in situ hybridization.

4. A cosmid vector for systematic chromosome walking.

5. Pig microsatellites isolated from cosmids revealing polymorphism and localized on chromosomes.

6. Localization of the porcine growth hormone gene to chromosome 12p1.2-->p1.5.

7. Mapping of human chromosome Xq28 by two-color fluorescence in situ hybridization of DNA sequences to interphase cell nuclei.

8. Rapid physical mapping of cloned DNA on banded mouse chromosomes by fluorescence in situ hybridization.

9. Localization of the pig luteinizing hormone/choriogonadotropin receptor gene (LHCGR) by radioactive and nonradioactive in situ hybridization.

1. Localization of beta-galactoside alpha2,6-sialyltransferase gene on pig chromosome 13q4.1.

2. Transcriptomic and nuclear architecture of immune cells after LPS activation.

3. Construction of a swine YAC library allowing an efficient recovery of unique and centromeric repeated sequences.

4. The cytogenetic map of the domestic pig.

5. Mapping of the melatonin receptor 1a (MTNR1A) gene in pigs, sheep, and cattle.

6. Anchorage of an unassigned linkage group to pig chromosome 10 with P1 clones.

7. Nuclear architecture of resting and LPS-stimulated porcine neutrophils by 3D FISH.

8. Human DNA polymerase eta is required for common fragile site stability during unperturbed DNA replication.

9. The PiGMaP consortium linkage map of the pig (Sus scrofa).

Review 10. The PiGMaP consortium cytogenetic map of the domestic pig (Sus scrofa domestica).