Literature DB >> 2369840

Rapid subchromosomal localization of cosmids by nonradioactive in situ hybridization.

T Kievits1, J G Dauwerse, J Wiegant, P Devilee, M H Breuning, C J Cornelisse, G J van Ommen, P L Pearson.   

Abstract

A rapid method for localizing large numbers of complete cosmids by nonradioactive in situ hybridization is described. The cosmids are nick translated in the presence of biotin-16-dUTP, incubated with an excess of sonicated human DNA, and used as a probe for in situ hybridization. Sites of hybridization are detected by successive treatments with FITC-labeled avidin and biotinylated anti-avidin antibody. Fifty-two cosmids were localized on chromosome 16 in 5 d relative to translocation breakpoints contained in two cell lines. Rapid identification of chromosome 16 was achieved by cohybridization with a chromosome 16-specific centromeric repeat probe.

Entities:  

Mesh：

Year:  1990        PMID： 2369840     DOI： 10.1159/000132913

Source DB:  PubMed          Journal:  Cytogenet Cell Genet        ISSN： 0301-0171


  32 in total

1.  Detection of Epstein-Barr virus in Hodgkin-Reed-Sternberg cells : no evidence for the persistence of integrated viral fragments inLatent membrane protein-1 (LMP-1)-negative classical Hodgkin's disease.

Authors:  A Staratschek-Jox; S Kotkowski; G Belge; T Rüdiger; J Bullerdiek; V Diehl; J Wolf
Journal:  Am J Pathol       Date:  2000-01       Impact factor: 4.307

2.  Mapping of two human homologs of a Drosophila heterochromatin protein gene to the X chromosome.

Authors:  W Reik; M A Leversha; N R Waterfield; P B Singh
Journal:  Mamm Genome       Date:  1992       Impact factor: 2.957

3.  A YAC contig across the fragile X site defines the region of fragility.

Authors:  M C Hirst; K Rack; Y Nakahori; A Roche; M V Bell; G Flynn; Z Christadoulou; R N MacKinnon; M Francis; A J Littler
Journal:  Nucleic Acids Res       Date:  1991-06-25       Impact factor: 16.971

4.  Use of 3' untranslated sequences of human cDNAs for rapid chromosome assignment and conversion to STSs: implications for an expression map of the genome.

Authors:  A S Wilcox; A S Khan; J A Hopkins; J M Sikela
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

5.  Mspl RFLP at the D5S122 locus tightly linked to APC.

Authors:  C Breukel; C Tops; E Ras; P M Khan
Journal:  Nucleic Acids Res       Date:  1991-02-11       Impact factor: 16.971

6.  Assignment of the gene coding for human cytochrome c oxidase subunit VIb to chromosome 19, band q13.1, by fluorescence in situ hybridisation.

Authors:  J W Taanman; A Y van der Veen; C Schrage; H de Vries; C H Buys
Journal:  Hum Genet       Date:  1991-07       Impact factor: 4.132

7.  Saturating the region of the polycystic kidney disease gene with NotI linking clones.

Authors:  H Himmelbauer; G G Germino; I Ceccherini; G Romeo; S T Reeders; A M Frischauf
Journal:  Am J Hum Genet       Date:  1991-02       Impact factor: 11.025

8.  Not para-, not peri-, but centric inversion of chromosome 12.

Authors:  A N Silahtaroglu; S Hacihanefioglu; G S Güven; A Cenani; J Wirth; N Tommerup; Z Tümer
Journal:  J Med Genet       Date:  1998-08       Impact factor: 6.318

9.  Human thrombopoietin: gene structure, cDNA sequence, expression, and chromosomal localization.

Authors:  D C Foster; C A Sprecher; F J Grant; J M Kramer; J L Kuijper; R D Holly; T E Whitmore; M D Heipel; L A Bell; A F Ching
Journal:  Proc Natl Acad Sci U S A       Date:  1994-12-20       Impact factor: 11.205

10.  DNA diagnosis of Prader-Willi and Angelman syndromes with the probe PW71 (D15S63).

Authors:  A M van den Ouweland; M N van der Est; E Wesby-van Swaay; T S Tijmensen; F J Los; J O Van Hemel; R C Hennekam; H J Meijers-Heijboer; M F Niermeijer; D J Halley
Journal:  Hum Genet       Date:  1995-05       Impact factor: 4.132
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