Literature DB >> 1417071

Measurement of S phase duration in human epidermis using cyclin immunostaining and 3H-thymidine pulse labelling.

E Berlingin1, M Heenen, P Galand.   

Abstract

Cyclin/PCNA (proliferating cell nuclear antigen) is a nuclear protein strongly associated with DNA replication sites. Under methanol fixation, cyclin immunostaining might serve as a specific marker for S-phase cells. Associated with single labelling with 3H-thymidine, this immunodetection represents an easy method for measuring S-phase duration. Its application to six samples of normal human epidermis allowed us to measure an S phase of 9.7 +/- 0.3 h, a result in fair agreement with a previous estimate of 10.2 h obtained from a classical double-labelling technique with two different concentrations of 3H-thymidine.

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Year:  1992        PMID： 1417071     DOI： 10.1007/bf00375801

Source DB:  PubMed          Journal:  Arch Dermatol Res        ISSN： 0340-3696            Impact factor:   3.017


  19 in total

1.  Autoantibody to a nuclear antigen in proliferating cells.

Authors:  K Miyachi; M J Fritzler; E M Tan
Journal:  J Immunol       Date:  1978-12       Impact factor: 5.422

2.  Cyclin/PCNA immunostaining as an alternative to tritiated thymidine pulse labelling for marking S phase cells in paraffin sections from animal and human tissues.

Authors:  P Galand; C Degraef
Journal:  Cell Tissue Kinet       Date:  1989-09

3.  Expression of proliferating cell nuclear antigen/cyclin in human keratinocytes.

Authors:  S Miyagawa; N Okada; Y Takasaki; T Iida; Y Kitano; K Yoshikawa; K Sakamoto; M L Steinberg
Journal:  J Invest Dermatol       Date:  1989-11       Impact factor: 8.551

4.  Cell population kinetics in human epidermis: in vitro autoradiographic study by double-labeling method.

Authors:  M Heenen; P Galand
Journal:  J Invest Dermatol       Date:  1971-06       Impact factor: 8.551

5.  Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67.

Authors:  J Gerdes; H Lemke; H Baisch; H H Wacker; U Schwab; H Stein
Journal:  J Immunol       Date:  1984-10       Impact factor: 5.422

6.  DNA-synthesizing cells in oral epithelium have a range of cell cycle durations: evidence from double-labelling studies using tritiated thymidine and bromodeoxyuridine.

Authors:  W J Hume
Journal:  Cell Tissue Kinet       Date:  1989-09

7.  Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication.

Authors:  H G Gratzner
Journal:  Science       Date:  1982-10-29       Impact factor: 47.728

8.  Expression of proliferating cell nuclear antigen (PCNA)/cyclin during the cell cycle.

Authors:  P Kurki; M Vanderlaan; F Dolbeare; J Gray; E M Tan
Journal:  Exp Cell Res       Date:  1986-09       Impact factor: 3.905

9.  Monoclonal antibodies to a nuclear protein (PCNA/cyclin) associated with DNA replication.

Authors:  K Ogata; P Kurki; J E Celis; R M Nakamura; E M Tan
Journal:  Exp Cell Res       Date:  1987-02       Impact factor: 3.905

10.  Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites.

Authors:  R Bravo; H Macdonald-Bravo
Journal:  J Cell Biol       Date:  1987-10       Impact factor: 10.539
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  1 in total

1.  Fixation requirements for the immunohistochemical reactivity of PCNA antibody PC10 on cryostat sections.

Authors:  H P Baum; J Reichrath; A Theobald; G Schock
Journal:  Histochem J       Date:  1994-12
  1 in total

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