Literature DB >> 7883726

Modulation of the allosteric equilibrium of yeast chorismate mutase by variation of a single amino acid residue.

R Graf1, Y Dubaquié, G H Braus.   

Abstract

Chorismate mutase (EC 5.4.99.5) from the yeast Saccharomyces cerevisiae is an allosteric enzyme which can be locked in its active R (relaxed) state by a single threonine-to-isoleucine exchange at position 226. Seven new replacements of residue 226 reveal that this position is able to direct the enzyme's allosteric equilibrium, without interfering with the catalytic constant or the affinity for the activator.

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Year:  1995        PMID： 7883726      PMCID： PMC176788          DOI： 10.1128/jb.177.6.1645-1648.1995

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  14 in total

1.  ON THE NATURE OF ALLOSTERIC TRANSITIONS: A PLAUSIBLE MODEL.

Authors:  J MONOD; J WYMAN; J P CHANGEUX
Journal:  J Mol Biol       Date:  1965-05       Impact factor: 5.469

2.  Site-directed mutagenesis using a double-stranded DNA fragment as a PCR primer.

Authors:  L B Giebel; R A Spritz
Journal:  Nucleic Acids Res       Date:  1990-08-25       Impact factor: 16.971

3.  Regulation of chorismate mutase activity of various yeast species by aromatic amino acids.

Authors:  R Bode; D Birnbaum
Journal:  Antonie Van Leeuwenhoek       Date:  1991-01       Impact factor: 2.271

4.  Routes to catalysis: structure of a catalytic antibody and comparison with its natural counterpart.

Authors:  M R Haynes; E A Stura; D Hilvert; I A Wilson
Journal:  Science       Date:  1994-02-04       Impact factor: 47.728

5.  Chorismate Mutase Isoenzymes from Selected Plants and Their Immunological Comparison with the Isoenzymes from Sorghum bicolor.

Authors:  B K Singh; S G Lonergan; E E Conn
Journal:  Plant Physiol       Date:  1986-07       Impact factor: 8.340

6.  Yeast allosteric chorismate mutase is locked in the activated state by a single amino acid substitution.

Authors:  T Schmidheini; H U Mösch; J N Evans; G Braus
Journal:  Biochemistry       Date:  1990-04-17       Impact factor: 3.162

7.  Cloning and expression in yeast of a higher plant chorismate mutase. Molecular cloning, sequencing of the cDNA and characterization of the Arabidopsis thaliana enzyme expressed in yeast.

Authors:  J Eberhard; H R Raesecke; J Schmid; N Amrhein
Journal:  FEBS Lett       Date:  1993-11-15       Impact factor: 4.124

8.  The crystal structure of allosteric chorismate mutase at 2.2-A resolution.

Authors:  Y Xue; W N Lipscomb; R Graf; G Schnappauf; G Braus
Journal:  Proc Natl Acad Sci U S A       Date:  1994-11-08       Impact factor: 11.205

Review 9.  Aromatic amino acid biosynthesis in the yeast Saccharomyces cerevisiae: a model system for the regulation of a eukaryotic biosynthetic pathway.

Authors:  G H Braus
Journal:  Microbiol Rev       Date:  1991-09

10.  A single point mutation results in a constitutively activated and feedback-resistant chorismate mutase of Saccharomyces cerevisiae.

Authors:  T Schmidheini; P Sperisen; G Paravicini; R Hütter; G Braus
Journal:  J Bacteriol       Date:  1989-03       Impact factor: 3.490
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  6 in total

Review 1.  Allosteric regulation of catalytic activity: Escherichia coli aspartate transcarbamoylase versus yeast chorismate mutase.

Authors:  K Helmstaedt; S Krappmann; G H Braus
Journal:  Microbiol Mol Biol Rev       Date:  2001-09       Impact factor: 11.056

2.  Coevolution of transcriptional and allosteric regulation at the chorismate metabolic branch point of Saccharomyces cerevisiae.

Authors:  S Krappmann; W N Lipscomb; G H Braus
Journal:  Proc Natl Acad Sci U S A       Date:  2000-12-05       Impact factor: 11.205

3.  A glutamate residue in the catalytic center of the yeast chorismate mutase restricts enzyme activity to acidic conditions.

Authors:  G Schnappauf; N Sträter; W N Lipscomb; G H Braus
Journal:  Proc Natl Acad Sci U S A       Date:  1997-08-05       Impact factor: 11.205

4.  Refined molecular hinge between allosteric and catalytic domain determines allosteric regulation and stability of fungal chorismate mutase.

Authors:  Kerstin Helmstaedt; Gabriele Heinrich; William N Lipscomb; Gerhard H Braus
Journal:  Proc Natl Acad Sci U S A       Date:  2002-05-07       Impact factor: 11.205

5.  HARO7 encodes chorismate mutase of the methylotrophic yeast Hansenula polymorpha and is derepressed upon methanol utilization.

Authors:  S Krappmann; R Pries; G Gellissen; M Hiller; G H Braus
Journal:  J Bacteriol       Date:  2000-08       Impact factor: 3.490

6.  Separation of inhibition and activation of the allosteric yeast chorismate mutase.

Authors:  G Schnappauf; W N Lipscomb; G H Braus
Journal:  Proc Natl Acad Sci U S A       Date:  1998-03-17       Impact factor: 11.205
  6 in total

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