Literature DB >> 2118627

Site-directed mutagenesis using a double-stranded DNA fragment as a PCR primer.

L B Giebel1, R A Spritz.   

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Year:  1990        PMID： 2118627      PMCID： PMC332011          DOI： 10.1093/nar/18.16.4947

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


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  3 in total

1.  The "megaprimer" method of site-directed mutagenesis.

Authors:  G Sarkar; S S Sommer
Journal:  Biotechniques       Date:  1990-04       Impact factor: 1.993

2.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

3.  Site-directed mutagenesis by overlap extension using the polymerase chain reaction.

Authors:  S N Ho; H D Hunt; R M Horton; J K Pullen; L R Pease
Journal:  Gene       Date:  1989-04-15       Impact factor: 3.688
  3 in total
  19 in total

1.  Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h.

Authors:  Joyce Chiu; Paul E March; Ryan Lee; Daniel Tillett
Journal:  Nucleic Acids Res       Date:  2004-12-07       Impact factor: 16.971

2.  Sequence requirements of the bidirectional yeast TRP4 mRNA 3'-end formation signal.

Authors:  C M Egli; K Düvel; N Trabesinger-Rüf; S Irniger; G H Braus
Journal:  Nucleic Acids Res       Date:  1997-01-15       Impact factor: 16.971

3.  Mutations in the YRB1 gene encoding yeast ran-binding-protein-1 that impair nucleocytoplasmic transport and suppress yeast mating defects.

Authors:  M Künzler; J Trueheart; C Sette; E Hurt; J Thorner
Journal:  Genetics       Date:  2001-03       Impact factor: 4.562

4.  Intestinal alkaline sphingomyelinase hydrolyses and inactivates platelet-activating factor by a phospholipase C activity.

Authors:  Jun Wu; Ake Nilsson; Bo A G Jönsson; Hanna Stenstad; William Agace; Yajun Cheng; Rui-Dong Duan
Journal:  Biochem J       Date:  2006-02-15       Impact factor: 3.857

5.  A glutamate residue in the catalytic center of the yeast chorismate mutase restricts enzyme activity to acidic conditions.

Authors:  G Schnappauf; N Sträter; W N Lipscomb; G H Braus
Journal:  Proc Natl Acad Sci U S A       Date:  1997-08-05       Impact factor: 11.205

6.  Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli.

Authors:  Liang Wang; Yoshifumi Hashimoto; Chen-Yu Tsao; James J Valdes; William E Bentley
Journal:  J Bacteriol       Date:  2005-03       Impact factor: 3.490

7.  Saturation mutagenesis of a polyadenylation signal reveals a hexanucleotide element essential for mRNA 3' end formation in Saccharomyces cerevisiae.

Authors:  S Irniger; G H Braus
Journal:  Proc Natl Acad Sci U S A       Date:  1994-01-04       Impact factor: 11.205

8.  Functional studies of human intestinal alkaline sphingomyelinase by deglycosylation and mutagenesis.

Authors:  Jun Wu; Gert H Hansen; Ake Nilsson; Rui-Dong Duan
Journal:  Biochem J       Date:  2005-02-15       Impact factor: 3.857

9.  Analysis of feedback-resistant anthranilate synthases from Saccharomyces cerevisiae.

Authors:  R Graf; B Mehmann; G H Braus
Journal:  J Bacteriol       Date:  1993-02       Impact factor: 3.490

10.  Different sequence elements are required for function of the cauliflower mosaic virus polyadenylation site in Saccharomyces cerevisiae compared with in plants.

Authors:  S Irniger; H Sanfaçon; C M Egli; G H Braus
Journal:  Mol Cell Biol       Date:  1992-05       Impact factor: 4.272
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