Literature DB >> 7859304

Molecular karyotype alterations induced by transformation in Aspergillus nidulans are mitotically stable.

X Xuei1, P L Skatrud.   

Abstract

Clamped homogeneous electric field (CHEF)-gel electrophoresis was used to define the electrophoretic molecular karyotype of Aspergillus nidulans strain OC-1 before and after protoplast-based genetic transformation. The transforming DNA caused alterations in the molecular karyotypes in all transformants examined. Rather dramatic changes were observed in karyotypes, including apparent chromosome loss, massive size alterations, and the appearance of large chromosomes. Changes in molecular karyotype were mitotically stable.

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Year:  1994        PMID： 7859304     DOI： 10.1007/bf00309551

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  9 in total

1.  Autonomously replicating plasmids and chromosome rearrangement during transformation of Nectria haematococca.

Authors:  H C Kistler; U Benny
Journal:  Gene       Date:  1992-08-01       Impact factor: 3.688

2.  Selectable genes for transformation of the fungal plant pathogen Glomerella cingulata f. sp. phaseoli (Colletotrichum lindemuthianum).

Authors:  R J Rodriguez; O C Yoder
Journal:  Gene       Date:  1987       Impact factor: 3.688

3.  Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph.

Authors:  D Cullen; S A Leong; L J Wilson; D J Henner
Journal:  Gene       Date:  1987       Impact factor: 3.688

4.  Electrophoretic karyotype of Aspergillus nidulans.

Authors:  H Brody; J Carbon
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205

5.  Transformation of Aspergillus nidulans by using a trpC plasmid.

Authors:  M M Yelton; J E Hamer; W E Timberlake
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

6.  Efficient isolation and mapping of rad genes of the fungus Coprinus cinereus using chromosome-specific libraries.

Authors:  M E Zolan; J R Crittenden; N K Heyler; L C Seitz
Journal:  Nucleic Acids Res       Date:  1992-08-11       Impact factor: 16.971

7.  Transformation of Aspergillus nidulans by the orotidine-5'-phosphate decarboxylase gene of Neurospora crassa.

Authors:  D J Ballance; F P Buxton; G Turner
Journal:  Biochem Biophys Res Commun       Date:  1983-04-15       Impact factor: 3.575

8.  Unusually large telomeric repeats in the yeast Candida albicans.

Authors:  M J McEachern; J B Hicks
Journal:  Mol Cell Biol       Date:  1993-01       Impact factor: 4.272

9.  Efficient integrative transformation of Cephalosporium acremonium.

Authors:  P L Skatrud; S W Queener; L G Carr; D L Fisher
Journal:  Curr Genet       Date:  1987       Impact factor: 3.886
  9 in total
  5 in total

Review 1.  Chromosome-length polymorphism in fungi.

Authors:  M E Zolan
Journal:  Microbiol Rev       Date:  1995-12

2.  Generation of large chromosomal deletions in koji molds Aspergillus oryzae and Aspergillus sojae via a loop-out recombination.

Authors:  Tadashi Takahashi; Feng Jie Jin; Misao Sunagawa; Masayuki Machida; Yasuji Koyama
Journal:  Appl Environ Microbiol       Date:  2008-10-24       Impact factor: 4.792

3.  Characterization of essential genes by parasexual genetics in the human fungal pathogen Aspergillus fumigatus: impact of genomic rearrangements associated with electroporation of DNA.

Authors:  Arnaud Firon; Anne Beauvais; Jean-Paul Latgé; Elisabeth Couvé; Marie-Claire Grosjean-Cournoyer; Christophe D'Enfert
Journal:  Genetics       Date:  2002-07       Impact factor: 4.562

4.  Integrative and replicative genetic transformation of Aureobasidium pullulans.

Authors:  S J Thornewell; R B Peery; P L Skatrud
Journal:  Curr Genet       Date:  1995-12       Impact factor: 3.886

5.  Transformation of Sordaria macrospora to hygromycin B resistance: characterization of transformants by electrophoretic karyotyping and tetrad analysis.

Authors:  M Walz; U Kück
Journal:  Curr Genet       Date:  1995-12       Impact factor: 3.886
  5 in total

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