Literature DB >> 783149

Isolation of temperature-sensitive mutants of R plasmid by in vitro mutagenesis with hydroxylamine.

T Hashimoto, M Sekiguchi.   

Abstract

Temperature-sensitive mutants were isolated from a tetracycline resistance plasmid, pSC101, after mutagenesis with hydroxylamine. They were divided into three classes according to their growth characteristics. They include mutants defective in the replication of plasmid deoxyribonucleic acid and strains having a mutation in the drug resistance gene.

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Year:  1976        PMID： 783149      PMCID： PMC232954          DOI： 10.1128/jb.127.3.1561-1563.1976

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  5 in total

1.  Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.

Authors:  E M Lederberg; S N Cohen
Journal:  J Bacteriol       Date:  1974-09       Impact factor: 3.490

2.  Construction of biologically functional bacterial plasmids in vitro.

Authors:  S N Cohen; A C Chang; H W Boyer; R B Helling
Journal:  Proc Natl Acad Sci U S A       Date:  1973-11       Impact factor: 11.205

3.  Isolation and characterization of a temperature-sensitive amber suppressor mutant of Escherichia coli K12.

Authors:  T Nagata; T Horiuchi
Journal:  Mol Gen Genet       Date:  1973

4.  Mutagenic treatment of double- and single-stranded DNA phages T4 ans S13 with hydroxylamine.

Authors:  I Tessman
Journal:  Virology       Date:  1968-06       Impact factor: 3.616

5.  Indirect selection of bacterial plasmids lacking identifiable phenotypic properties.

Authors:  F J Kretschmer; A C Chang; S N Cohen
Journal:  J Bacteriol       Date:  1975-10       Impact factor: 3.490
  5 in total
  34 in total

1.  Construction of plasmid-free derivatives of Salmonella typhimurium LT2 using temperature-sensitive mutants of pKZ1 for displacement of the resident plasmid, pSLT.

Authors:  R A Kelln; L G Lintott
Journal:  Mol Gen Genet       Date:  1990-07

2.  Involvement of integration host factor (IHF) in maintenance of plasmid pSC101 in Escherichia coli: characterization of pSC101 mutants that replicate in the absence of IHF.

Authors:  D P Biek; S N Cohen
Journal:  J Bacteriol       Date:  1989-04       Impact factor: 3.490

3.  Primary structure of the essential replicon of the plasmid pSC101.

Authors:  C Vocke; D Bastia
Journal:  Proc Natl Acad Sci U S A       Date:  1983-11       Impact factor: 11.205

4.  Transfer of drug resistance factors to the dimorphic bacterium Caulobacter crescentus.

Authors:  B Ely
Journal:  Genetics       Date:  1979-03       Impact factor: 4.562

5.  Isolation and characterization of replication-deficient mutants of ColE1 plasmids.

Authors:  T Hashimoto-Gotoh; J Inselburg
Journal:  J Bacteriol       Date:  1979-08       Impact factor: 3.490

6.  Effect of dna mutations on the replication of plasmid pSC101 in Escherichia coli K-12.

Authors:  K Hasunuma; M Sekiguchi
Journal:  J Bacteriol       Date:  1979-03       Impact factor: 3.490

7.  Genetic analysis of the Saccharomyces cerevisiae RHO3 gene, encoding a rho-type small GTPase, provides evidence for a role in bud formation.

Authors:  J Imai; A Toh-e; Y Matsui
Journal:  Genetics       Date:  1996-02       Impact factor: 4.562

8.  Mutation in the plasmid pUB110 Rep protein affects termination of rolling circle replication.

Authors:  V E Bidnenko; A Gruss; S D Ehrlich
Journal:  J Bacteriol       Date:  1993-09       Impact factor: 3.490

9.  [Genetic analysis of sacB, the structural gene of a secreted enzyme, levansucrase of Bacillus subtilis Marburg].

Authors:  M Steinmetz; D Le Coq; H B Djemia; P Gay
Journal:  Mol Gen Genet       Date:  1983

10.  Mutations in GCR1, a transcriptional activator of Saccharomyces cerevisiae glycolytic genes, function as suppressors of gcr2 mutations.

Authors:  H Uemura; Y Jigami
Journal:  Genetics       Date:  1995-02       Impact factor: 4.562
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