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Literature DB >> 7829682

Rapid and simple method for preparation of genomic DNA from easily obtainable clotted blood.

Abstract

A method was developed for the preparation of genomic DNA from clotted blood that is usually discarded after extraction, for other laboratory tests. The method, which involves proteinase K digestion, salt/chloroform extraction and 90% ethanol precipitation of DNA from clotted blood, is rapid, simple, and easy because it does not impose an extra burden on the patient.

Entities: Chemical Species

Mesh：

Substances：
DNA

Year: 1994 PMID： 7829682 PMCID： PMC503071 DOI： 10.1136/jcp.47.11.1043

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

3 in total

1. Improved method for direct PCR amplification from whole blood.

Authors: J McCusker; M T Dawson; D Noone; F Gannon; T Smith
Journal: Nucleic Acids Res Date: 1992-12-25 Impact factor: 16.971

2. An efficient salt-chloroform extraction of DNA from blood and tissues.

Authors: R Müllenbach; P J Lagoda; C Welter
Journal: Trends Genet Date: 1989-12 Impact factor: 11.639

3. PCR based diagnosis in the presence of 8% (v/v) blood.

Authors: M Panaccio; A Lew
Journal: Nucleic Acids Res Date: 1991-03-11 Impact factor: 16.971

3 in total

14 in total

1. Mutation in the Sp1 motif of the bovine leptin gene affects its expression.

Authors: Tatiana Adamowicz; Krzysztof Flisikowski; Rafał Starzyński; Lech Zwierzchowski; Marek Switoński
Journal: Mamm Genome Date: 2006-01-13 Impact factor: 2.957

2. A new SNP in the 3'UTR region of the bovine calpain small subunit (CAPNS1) gene.

Authors: E Juszczuk-Kubiak; K Flisikowski; K Wicińska
Journal: Mol Biol Rep Date: 2009-08-02 Impact factor: 2.316

3. Sensitive and universal method for microbial DNA extraction from blood products.

Authors: N Golbang; J P Burnie; P E Klapper; A Bostock; P Williamson
Journal: J Clin Pathol Date: 1996-10 Impact factor: 3.411

4. A novel polymorphisms in intron 12 of the bovine calpastatin gene.

Authors: Edyta Juszczuk-Kubiak; Joanna Wyszyńska-Koko; Krystyna Wicińska; Stanisław Rosochacki
Journal: Mol Biol Rep Date: 2007-01-09 Impact factor: 2.316

5. Maternal care according to offspring sex and maternal physical condition in mantled howler monkeys (Alouatta palliata).

Authors: Amalia de la Torre; Alejandro Coyohua Fuentes; Ariadna Rangel Negrín; Daniel A Velarde Garcéz; Domingo Canales Espinosa; Patricia Cervantes Acosta; Pedro Américo D Dias
Journal: Primates Date: 2021-02-01 Impact factor: 2.163

Rapid and simple method for preparation of genomic DNA from easily obtainable clotted blood.

1. Improved method for direct PCR amplification from whole blood.

2. An efficient salt-chloroform extraction of DNA from blood and tissues.

3. PCR based diagnosis in the presence of 8% (v/v) blood.

1. Mutation in the Sp1 motif of the bovine leptin gene affects its expression.

2. A new SNP in the 3'UTR region of the bovine calpain small subunit (CAPNS1) gene.

3. Sensitive and universal method for microbial DNA extraction from blood products.

4. A novel polymorphisms in intron 12 of the bovine calpastatin gene.

5. Maternal care according to offspring sex and maternal physical condition in mantled howler monkeys (Alouatta palliata).

6. A practical and novel method to extract genomic DNA from blood collection kits for plasma protein preservation.

7. Expression in promoter variant of the ERalpha gene in bos taurus liver.

8. Hsa-miRNA-23a-3p promotes atherogenesis in a novel mouse model of atherosclerosis.

9. A note on the organization and expression of β-defensin genes in Polish goats.

10. Allelic gene expression imbalance of bovine IGF2, LEP and CCL2 genes in liver, kidney and pituitary.