Literature DB >> 7819480

A liquid crystalline phase in spermidine-condensed DNA.

J L Sikorav1, J Pelta, F Livolant.   

Abstract

Over a large range of salt and spermidine concentrations, short DNA fragments precipitated by spermidine (a polyamine) sediment in a pellet from a dilute isotropic supernatant. We report here that the DNA-condensed phase consists of a cholesteric liquid crystal in equilibrium with a more concentrated phase. These results are discussed according to Flory's theory for the ordering of rigid polymers. The liquid crystal described here corresponds to an ordering in the presence of attractive interactions, in contrast with classical liquid crystalline DNA. Polyamines are often used in vitro to study the functional properties of DNA. We suggest that the existence of a liquid crystalline state in spermidine-condensed DNA is relevant to these studies.

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Year:  1994        PMID： 7819480      PMCID： PMC1225502          DOI： 10.1016/S0006-3495(94)80640-X

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  25 in total

1.  DNA packing in single crystals inferred from freeze-fracture-etch replicas.

Authors:  L S Lerman; L S Wilkerson; J H Venable; B H Robinson
Journal:  J Mol Biol       Date:  1976-12       Impact factor: 5.469

2.  Catalysis of DNA reassociation by the Escherichia coli DNA binding protein: A polyamine-dependent reaction.

Authors:  C Christiansen; R L Baldwin
Journal:  J Mol Biol       Date:  1977-09-25       Impact factor: 5.469

Review 3.  The molecular theory of polyelectrolyte solutions with applications to the electrostatic properties of polynucleotides.

Authors:  G S Manning
Journal:  Q Rev Biophys       Date:  1978-05       Impact factor: 5.318

4.  Counterion-induced condesation of deoxyribonucleic acid. a light-scattering study.

Authors:  R W Wilson; V A Bloomfield
Journal:  Biochemistry       Date:  1979-05-29       Impact factor: 3.162

5.  Chromosomal analogues: long-range order in psi-condensed DNA.

Authors:  L S Lerman
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1974

6.  Spermine-DNA complexes build up metastable structures. Small-angle X-ray scattering and circular dichroism studies.

Authors:  M Becker; R Misselwitz; H Damaschun; G Damaschun; D Zirwer
Journal:  Nucleic Acids Res       Date:  1979-11-10       Impact factor: 16.971

7.  Catenation of DNA rings by topoisomerases. Mechanism of control by spermidine.

Authors:  M A Krasnow; N R Cozzarelli
Journal:  J Biol Chem       Date:  1982-03-10       Impact factor: 5.157

8.  DNA condensation with polyamines. II. Electron microscopic studies.

Authors:  D K Chattoraj; L C Gosule; A Schellman
Journal:  J Mol Biol       Date:  1978-05-25       Impact factor: 5.469

9.  Contribution of light scattering to the circular dichroism of deoxyribonucleic acid films, deoxyribonucleic acid-polylysine complexes, and deoxyribonucleic acid particles in ethanolic buffers.

Authors:  M F Maestre; C Reich
Journal:  Biochemistry       Date:  1980-11-11       Impact factor: 3.162

Review 10.  Multienzyme systems of DNA replication.

Authors:  R Schekman; A Weiner; A Kornberg
Journal:  Science       Date:  1974-12-13       Impact factor: 47.728
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  16 in total

1.  Regulated phase transitions of bacterial chromatin: a non-enzymatic pathway for generic DNA protection.

Authors:  D Frenkiel-Krispin; S Levin-Zaidman; E Shimoni; S G Wolf; E J Wachtel; T Arad; S E Finkel; R Kolter; A Minsky
Journal:  EMBO J       Date:  2001-03-01       Impact factor: 11.598

2.  Formation of impeller-like helical DNA-silica complexes by polyamines induced chiral packing.

Authors:  Ben Liu; Lu Han; Shunai Che
Journal:  Interface Focus       Date:  2012-02-22       Impact factor: 3.906

3.  The observation of the local ordering characteristics of spermidine-condensed DNA: atomic force microscopy and polarizing microscopy studies.

Authors:  Z Lin; C Wang; X Feng; M Liu; J Li; C Bai
Journal:  Nucleic Acids Res       Date:  1998-07-01       Impact factor: 16.971

4.  Precipitation of DNA by polyamines: a polyelectrolyte behavior.

Authors:  E Raspaud; M Olvera de la Cruz; J L Sikorav; F Livolant
Journal:  Biophys J       Date:  1998-01       Impact factor: 4.033

5.  DNA mesophases induced by spermidine: structural properties and biological implications.

Authors:  J Pelta; D Durand; J Doucet; F Livolant
Journal:  Biophys J       Date:  1996-07       Impact factor: 4.033

6.  Condensation and localization of the partitioning protein ParB on the bacterial chromosome.

Authors:  Chase P Broedersz; Xindan Wang; Yigal Meir; Joseph J Loparo; David Z Rudner; Ned S Wingreen
Journal:  Proc Natl Acad Sci U S A       Date:  2014-06-03       Impact factor: 11.205

7.  Ligand-induced DNA condensation: choosing the model.

Authors:  Vladimir B Teif
Journal:  Biophys J       Date:  2005-08-05       Impact factor: 4.033

8.  Charge inversion, condensation and decondensation of DNA and polystyrene sulfonate by polyethylenimine.

Authors:  V Mengarelli; L Auvray; D Pastré; M Zeghal
Journal:  Eur Phys J E Soft Matter       Date:  2011-11-25       Impact factor: 1.890

9.  Interhelical spacing in liquid crystalline spermine and spermidine-DNA precipitates.

Authors:  E Raspaud; D Durand; F Livolant
Journal:  Biophys J       Date:  2004-10-15       Impact factor: 4.033

10.  Polyamine structural effects on the induction and stabilization of liquid crystalline DNA: potential applications to DNA packaging, gene therapy and polyamine therapeutics.

Authors:  M Saminathan; Thresia Thomas; Akira Shirahata; C K S Pillai; T J Thomas
Journal:  Nucleic Acids Res       Date:  2002-09-01       Impact factor: 16.971
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