Jin-Fang Wu1, Lu Xia, Yu-Xin Liu, Xin Zhang, Chen Li, Ming-Li Li. 1. Department of Obstetrics and Gynecology, Xi'an Jiaotong University Medical School Second Affiliated Hospital, Xi Wu Road 157, Xi'an City, Xi'an, 710004, China. wujinfang234@163.com
Abstract
PURPOSE: This study investigated the allelic frequency of 4 short terminal repeats (STRs) in Han and She populations from eastern China. METHODS: DNA samples from Han (N = 110) and She (N = 110) healthy subjects were amplified using PCR and primers specific for the STRs. D21S11 and D21S1446 (chromosome 21) and D18S865 and D18S535 (chromosome 18). The frequency and homozygocity of different STRs were evaluated. RESULTS: For both populations, D21S11 and D18S535 were more polymorphic than D21S1446 and D18S865, and the D21S1446 1 allele was very common (48.6% and 55.0% for the Han and She groups, respectively).The Han and She groups significantly differed from one another in respect to the distribution of D21S11, D18S865, and D18S535 allelic frequency (all P -values ≤ 0.48) but had a similar allelic distribution for D21S1446 (P = 0.106). D21S1446 was found to be homozygous about 50% of the time which was significantly greater compared to the other STRs analyzed. CONCLUSION: This study found that the Han and She differ genetically at 3 autosomal STRs located on chromosome 18 and 21. The STR D21S1146 was often homozygous within these groups, indicating it would be poor marker for analyzing trisomy within these populations.
PURPOSE: This study investigated the allelic frequency of 4 short terminal repeats (STRs) in Han and She populations from eastern China. METHODS: DNA samples from Han (N = 110) and She (N = 110) healthy subjects were amplified using PCR and primers specific for the STRs. D21S11 and D21S1446 (chromosome 21) and D18S865 and D18S535 (chromosome 18). The frequency and homozygocity of different STRs were evaluated. RESULTS: For both populations, D21S11 and D18S535 were more polymorphic than D21S1446 and D18S865, and the D21S1446 1 allele was very common (48.6% and 55.0% for the Han and She groups, respectively).The Han and She groups significantly differed from one another in respect to the distribution of D21S11, D18S865, and D18S535 allelic frequency (all P -values ≤ 0.48) but had a similar allelic distribution for D21S1446 (P = 0.106). D21S1446 was found to be homozygous about 50% of the time which was significantly greater compared to the other STRs analyzed. CONCLUSION: This study found that the Han and She differ genetically at 3 autosomal STRs located on chromosome 18 and 21. The STR D21S1146 was often homozygous within these groups, indicating it would be poor marker for analyzing trisomy within these populations.
Authors: Noah A Rosenberg; Jonathan K Pritchard; James L Weber; Howard M Cann; Kenneth K Kidd; Lev A Zhivotovsky; Marcus W Feldman Journal: Science Date: 2002-12-20 Impact factor: 47.728
Authors: N E Lamb; S B Freeman; A Savage-Austin; D Pettay; L Taft; J Hersey; Y Gu; J Shen; D Saker; K M May; D Avramopoulos; M B Petersen; A Hallberg; M Mikkelsen; T J Hassold; S L Sherman Journal: Nat Genet Date: 1996-12 Impact factor: 38.330
Authors: Rossa W K Chiu; K C Allen Chan; Yuan Gao; Virginia Y M Lau; Wenli Zheng; Tak Y Leung; Chris H F Foo; Bin Xie; Nancy B Y Tsui; Fiona M F Lun; Benny C Y Zee; Tze K Lau; Charles R Cantor; Y M Dennis Lo Journal: Proc Natl Acad Sci U S A Date: 2008-12-10 Impact factor: 11.205