Literature DB >> 7789947

Coverage of chromosome 6 by chromosome microdissection: generation of 14 subregion-specific probes.

X Y Guan1, P S Meltzer, A C Burgess, J M Trent.   

Abstract

Human chromosome 6 has been subdivided by chromosome microdissection into 14 unique regions. Following microdissection, polymerase chain reaction (PCR) amplification of dissected DNA was performed using a universal primer to generate subregion-specific probes that provided complete coverage of chromosome 6. All 16 microdissections have been regionally assigned along chromosome 6 by fluorescence in situ hybridization (FISH) using biotin-labeled dissected DNA hybridized to G-banded normal metaphase chromosomes. These probes can be used as region-specific paints to generate unique "bar codes" and for analysis of chromosome alterations involving chromosome 6 that are unidentifiable by conventional banding analysis.

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Year:  1995        PMID： 7789947     DOI： 10.1007/bf00209479

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  16 in total

1.  A method for the rapid sequence-independent amplification of microdissected chromosomal material.

Authors:  S K Bohlander; R Espinosa; M M Le Beau; J D Rowley; M O Díaz
Journal:  Genomics       Date:  1992-08       Impact factor: 5.736

2.  Chromosome microdissection and cloning in human genome and genetic disease analysis.

Authors:  F T Kao; J W Yu
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-01       Impact factor: 11.205

3.  Isolation of region-specific cosmids from chromosome 5 by hybridization with microdissection clones.

Authors:  D L Saltman; G M Dolganov; B S Pearce; S S Kuo; P J Callahan; M L Cleary; M Lovett
Journal:  Nucleic Acids Res       Date:  1992-03-25       Impact factor: 16.971

4.  Degenerate oligonucleotide-primed PCR: general amplification of target DNA by a single degenerate primer.

Authors:  H Telenius; N P Carter; C E Bebb; M Nordenskjöld; B A Ponder; A Tunnacliffe
Journal:  Genomics       Date:  1992-07       Impact factor: 5.736

5.  Laser microdissection of the fragile X region: identification of cosmid clones and of conserved sequences in this region.

Authors:  M Djabali; C Nguyen; I Biunno; B A Oostra; M G Mattei; J E Ikeda; B R Jordan
Journal:  Genomics       Date:  1991-08       Impact factor: 5.736

6.  Chromosome-band-specific painting: chromosome in situ suppression hybridization using PCR products from a microdissected chromosome band as a probe pool.

Authors:  H X Deng; K Yoshiura; R W Dirks; N Harada; T Hirota; K Tsukamoto; Y Jinno; N Niikawa
Journal:  Hum Genet       Date:  1992-04       Impact factor: 4.132

7.  Chromosomal bar codes produced by multicolor fluorescence in situ hybridization with multiple YAC clones and whole chromosome painting probes.

Authors:  C Lengauer; M R Speicher; S Popp; A Jauch; M Taniwaki; R Nagaraja; H C Riethman; H Donis-Keller; M D'Urso; D Schlessinger
Journal:  Hum Mol Genet       Date:  1993-05       Impact factor: 6.150

8.  Microdissection of human chromosomal regions 8q23.3-q24.11 and 2q33-qter: construction of DNA libraries and isolation of their clones.

Authors:  T Hirota; K Tsukamoto; H X Deng; K Yoshiura; T Ohta; T Tohma; T Kibe; N Harada; Y Jinno; N Niikawa
Journal:  Genomics       Date:  1992-06       Impact factor: 5.736

9.  Construction and characterization of band-specific DNA libraries.

Authors:  H J Lüdecke; G Senger; U Claussen; B Horsthemke
Journal:  Hum Genet       Date:  1990-05       Impact factor: 4.132

10.  Mutations of a mutS homolog in hereditary nonpolyposis colorectal cancer.

Authors:  F S Leach; N C Nicolaides; N Papadopoulos; B Liu; J Jen; R Parsons; P Peltomäki; P Sistonen; L A Aaltonen; M Nyström-Lahti
Journal:  Cell       Date:  1993-12-17       Impact factor: 41.582
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  7 in total

1.  Disclosure of five breakpoints in a complex chromosome rearrangement by microdissection and FISH.

Authors:  J J Engelen; W J Loots; J C Albrechts; P C Motoh; J P Fryns; A J Hamers; J P Geraedts
Journal:  J Med Genet       Date:  1996-07       Impact factor: 6.318

2.  Regional and temporal specialization in the nucleus: a transcriptionally-active nuclear domain rich in PTF, Oct1 and PIKA antigens associates with specific chromosomes early in the cell cycle.

Authors:  A Pombo; P Cuello; W Schul; J B Yoon; R G Roeder; P R Cook; S Murphy
Journal:  EMBO J       Date:  1998-03-16       Impact factor: 11.598

3.  Separate and variably shaped chromosome arm domains are disclosed by chromosome arm painting in human cell nuclei.

Authors:  S Dietzel; A Jauch; D Kienle; G Qu; H Holtgreve-Grez; R Eils; C Münkel; M Bittner; P S Meltzer; J M Trent; T Cremer
Journal:  Chromosome Res       Date:  1998-01       Impact factor: 5.239

4.  Region-specific YAC banding and painting probes for comparative genome mapping: implications for the evolution of human chromosome 2.

Authors:  T Haaf; P Bray-Ward
Journal:  Chromosoma       Date:  1996-06       Impact factor: 4.316

5.  Multiplex-FISH for pre- and postnatal diagnostic applications.

Authors:  S Uhrig; S Schuffenhauer; C Fauth; A Wirtz; C Daumer-Haas; C Apacik; M Cohen; J Müller-Navia; T Cremer; J Murken; M R Speicher
Journal:  Am J Hum Genet       Date:  1999-08       Impact factor: 11.025

6.  'Chromosomal Rainbows' Detect Oncogenic Rearrangements of Signaling Molecules in Thyroid Tumors.

Authors:  Benjamin O'Brien; Gregg H Jossart; Yuko Ito; Karin M Greulich-Bode; Jingly F Weier; Santiago Munne; Orlo H Clark; Heinz-Ulrich G Weier
Journal:  Open Cell Signal J       Date:  2010

7.  Interstitial deletion of chromosome 6q: precise definition of the breakpoints by microdissection, DNA amplification, and reverse painting.

Authors:  N Rubtsov; G Senger; H Kuzcera; A Neumann; C Kelbova; K Junker; V Beensen; U Claussen
Journal:  Hum Genet       Date:  1996-06       Impact factor: 4.132
  7 in total

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