Literature DB >> 2159949

Construction and characterization of band-specific DNA libraries.

H J Lüdecke1, G Senger, U Claussen, B Horsthemke.   

Abstract

A universally primed polymerase chain reaction was developed to amplify DNA dissected from GTG-banded human chromosomes. The amplification products are cloned into plasmid vectors, which allow the rapid characterization of recombinant clones. Starting from 20-40 chromosome fragments, several thousand independent clones detecting single-copy sequences can be obtained. Although these libraries comprise only a few percent of the dissected DNA, they provide narrowly spaced anchor clones for the molecular characterization of chromosome bands and the identification of gene sequences. Here we describe the construction and characterization of DNA libraries for the Langer-Giedion syndrome chromosome region (LGCR, 8q23-24.1), Wilms tumor chromosome region 1 (WT1, 11p13), Prader-Willi syndrome/Angelman syndrome chromosome region (PWCR/ANCR, 15q11.2-12), meningioma chromosome region (MGCR, 22q12-13), and fragile X chromosome region (FRAXA, Xq27.3).

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Year:  1990        PMID： 2159949     DOI： 10.1007/BF00210800

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  17 in total

1.  Microdissection of banded human chromosomes.

Authors:  G Senger; H J Lüdecke; B Horsthemke; U Claussen
Journal:  Hum Genet       Date:  1990-05       Impact factor: 4.132

2.  A model to describe the size distribution of mammalian genomic fragments recovered by microcloning.

Authors:  S D Brown; A J Greenfield
Journal:  Gene       Date:  1987       Impact factor: 3.688

3.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

4.  Microcloning of mammalian metaphase chromosomes.

Authors:  J E Edström; R Kaiser; D Röhme
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

5.  Base sequence studies of 300 nucleotide renatured repeated human DNA clones.

Authors:  P L Deininger; D J Jolly; C M Rubin; T Friedmann; C W Schmid
Journal:  J Mol Biol       Date:  1981-09-05       Impact factor: 5.469

6.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

7.  A model for restriction fragment length distributions.

Authors:  D T Bishop; J A Williamson; M H Skolnick
Journal:  Am J Hum Genet       Date:  1983-09       Impact factor: 11.025

8.  Isolation of molecular probes associated with the chromosome 15 instability in the Prader-Willi syndrome.

Authors:  T A Donlon; M Lalande; A Wyman; G Bruns; S A Latt
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205

9.  Microdissection of the Prader-Willi syndrome chromosome region and identification of potential gene sequences.

Authors:  K Buiting; M Neumann; H J Lüdecke; G Senger; U Claussen; J Antich; E Passarge; B Horsthemke
Journal:  Genomics       Date:  1990-03       Impact factor: 5.736

10.  Polymer-stimulated ligation: enhanced blunt- or cohesive-end ligation of DNA or deoxyribooligonucleotides by T4 DNA ligase in polymer solutions.

Authors:  B H Pheiffer; S B Zimmerman
Journal:  Nucleic Acids Res       Date:  1983-11-25       Impact factor: 16.971
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  18 in total

Review 1.  Long-range walking techniques in positional cloning strategies.

Authors:  L Stubbs
Journal:  Mamm Genome       Date:  1992       Impact factor: 2.957

2.  The development of chromosome microdissection and microcloning technique and its applications in genomic research.

Authors:  Ruo-Nan Zhou; Zan-Min Hu
Journal:  Curr Genomics       Date:  2007-03       Impact factor: 2.236

3.  Construction of an RFLP map of barley.

Authors:  A Graner; A Jahoor; J Schondelmaier; H Siedler; K Pillen; G Fischbeck; G Wenzel; R G Herrmann
Journal:  Theor Appl Genet       Date:  1991-12       Impact factor: 5.699

4.  Chromosome-band-specific painting: chromosome in situ suppression hybridization using PCR products from a microdissected chromosome band as a probe pool.

Authors:  H X Deng; K Yoshiura; R W Dirks; N Harada; T Hirota; K Tsukamoto; Y Jinno; N Niikawa
Journal:  Hum Genet       Date:  1992-04       Impact factor: 4.132

5.  Microdissection of banded human chromosomes.

Authors:  G Senger; H J Lüdecke; B Horsthemke; U Claussen
Journal:  Hum Genet       Date:  1990-05       Impact factor: 4.132

6.  Somatic cell hybrid and long-range physical mapping of 11p13 microdissected genomic clones.

Authors:  L M Davis; G Senger; H J Lüdecke; U Claussen; B Horsthemke; S S Zhang; B Metzroth; K Hohenfellner; B Zabel; T B Shows
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

7.  Isolation of DNA from the centromere of human chromosome 7 by microdissection.

Authors:  F Behrens; U Claussen; L M Iyer; E D Green; B Horsthemke; R Williamson; C Huxley; C Coutelle
Journal:  Chromosome Res       Date:  1997-06       Impact factor: 5.239

8.  Chromosome microdissection and microcloning.

Authors:  Y Saitoh; J E Ikeda
Journal:  Chromosome Res       Date:  1997-04       Impact factor: 5.239

9.  Gene mapping from a bovine 1;29 DNA library prepared with chromosome microdissection.

Authors:  S M Schmutz; T G Berryere; J S Moker; T D Thue; D C Winkelman
Journal:  Mamm Genome       Date:  1994-03       Impact factor: 2.957

10.  A region-specific microdissection library for human chromosome 2p23-p25 and the analysis of an interstitial deletion of 2p23.3-p25.1.

Authors:  J Yu; J Qi; S Tong; F T Kao
Journal:  Hum Genet       Date:  1994-05       Impact factor: 4.132
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