Literature DB >> 7765885

Acinetobacter calcoaceticus liberates chromosomal DNA during induction of competence by cell lysis.

R Palmen1, K J Hellingwerf.   

Abstract

A transformation assay was used to assay the amount of DNA present in the extracellular medium of a growing culture of Acinetobacter calcoaceticus. It was observed that small amounts of DNA were liberated during the entire exponential growth phase in a batch culture. Release of DNA could be fully accounted for by lysis of cells. Lysis was quantified via simultaneous measurement of beta-galactosidase activity of cells and supernatant, with a strain that contained a plasmid (pAPA100) with lacZ under control of a constitutive beta-lactamase promoter. In conclusion, no evidence could be obtained indicating that Acinetobacter calcoaceticus actively excretes DNA, to be used for DNA exchange.

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Year:  1995        PMID： 7765885     DOI： 10.1007/BF00294516

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  14 in total

1.  Characterization of transformation-deficient mutants of Acinetobacter calcoaceticus.

Authors:  R Palmen; B Vosman; R Kok; J R van der Zee; K J Hellingwerf
Journal:  Mol Microbiol       Date:  1992-07       Impact factor: 3.501

Review 2.  Uses of transposons with emphasis on Tn10.

Authors:  N Kleckner; J Bender; S Gottesman
Journal:  Methods Enzymol       Date:  1991       Impact factor: 1.600

3.  Interspecies transformation of Acinetobacter: genetic evidence for a ubiquitous genus.

Authors:  E Juni
Journal:  J Bacteriol       Date:  1972-11       Impact factor: 3.490

4.  Dynamics of extracellular DNA in the marine environment.

Authors:  J H Paul; W H Jeffrey; M F DeFlaun
Journal:  Appl Environ Microbiol       Date:  1987-01       Impact factor: 4.792

5.  Structural plasmid instability in Bacillus subtilis: effect of direct and inverted repeats.

Authors:  B P Peeters; J H de Boer; S Bron; G Venema
Journal:  Mol Gen Genet       Date:  1988-06

6.  Transfer of Haemophilus influenzae chromosomal genes by cell-to-cell contact.

Authors:  W L Albritton; J K Setlow; L Slaney
Journal:  J Bacteriol       Date:  1982-12       Impact factor: 3.490

7.  Analysis and nucleotide sequence of an origin of DNA replication in Acinetobacter calcoaceticus and its use for Escherichia coli shuttle plasmids.

Authors:  M Hunger; R Schmucker; V Kishan; W Hillen
Journal:  Gene       Date:  1990-03-01       Impact factor: 3.688

8.  Diphenylamine-colorimetric method for DNA assay: a shortened procedure by incubating samples at 50 degrees C.

Authors:  G J Gendimenico; P L Bouquin; K M Tramposch
Journal:  Anal Biochem       Date:  1988-08-15       Impact factor: 3.365

9.  Release of transforming plasmid and chromosomal DNA from two cultured soil bacteria.

Authors:  M G Lorenz; D Gerjets; W Wackernagel
Journal:  Arch Microbiol       Date:  1991       Impact factor: 2.552

10.  Growth-phase-dependent expression of the lipolytic system of Acinetobacter calcoaceticus BD413: cloning of a gene encoding one of the esterases.

Authors:  R G Kok; V M Christoffels; B Vosman; K J Hellingwerf
Journal:  J Gen Microbiol       Date:  1993-10
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  7 in total

1.  DNA as an adhesin: Bacillus cereus requires extracellular DNA to form biofilms.

Authors:  Sébastien Vilain; Jakobus M Pretorius; Jacques Theron; Volker S Brözel
Journal:  Appl Environ Microbiol       Date:  2009-02-27       Impact factor: 4.792

2.  Gene transfer potential of outer membrane vesicles of Acinetobacter baylyi and effects of stress on vesiculation.

Authors:  Shweta Fulsundar; Klaus Harms; Gøril E Flaten; Pål J Johnsen; Balu Ananda Chopade; Kaare M Nielsen
Journal:  Appl Environ Microbiol       Date:  2014-03-21       Impact factor: 4.792

3.  Natural transformation of Acinetobacter sp. strain BD413 with cell lysates of Acinetobacter sp., Pseudomonas fluorescens, and Burkholderia cepacia in soil microcosms.

Authors:  K M Nielsen; K Smalla; J D van Elsas
Journal:  Appl Environ Microbiol       Date:  2000-01       Impact factor: 4.792

4.  Global regulator H-NS and lipoprotein NlpI influence production of extracellular DNA in Escherichia coli.

Authors:  Viviana Sanchez-Torres; Toshinari Maeda; Thomas K Wood
Journal:  Biochem Biophys Res Commun       Date:  2010-09-15       Impact factor: 3.575

5.  Genome instability mediates the loss of key traits by Acinetobacter baylyi ADP1 during laboratory evolution.

Authors:  Brian A Renda; Aurko Dasgupta; Dacia Leon; Jeffrey E Barrick
Journal:  J Bacteriol       Date:  2014-12-15       Impact factor: 3.490

Review 6.  Mechanisms and Regulation of Extracellular DNA Release and Its Biological Roles in Microbial Communities.

Authors:  Alejandra L Ibáñez de Aldecoa; Olga Zafra; José E González-Pastor
Journal:  Front Microbiol       Date:  2017-07-26       Impact factor: 5.640

7.  DNA builds and strengthens the extracellular matrix in Myxococcus xanthus biofilms by interacting with exopolysaccharides.

Authors:  Wei Hu; Lina Li; Shivani Sharma; Jing Wang; Ian McHardy; Renate Lux; Zhe Yang; Xuesong He; James K Gimzewski; Yuezhong Li; Wenyuan Shi
Journal:  PLoS One       Date:  2012-12-26       Impact factor: 3.240
  7 in total

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