Literature DB >> 3189801

Diphenylamine-colorimetric method for DNA assay: a shortened procedure by incubating samples at 50 degrees C.

G J Gendimenico1, P L Bouquin, K M Tramposch.   

Abstract

We describe a modified procedure of the diphenylamine-colorimetric method for assay of DNA that is shorter than the procedure described by Burton. This improvement is achieved by raising the sample incubation temperature to 50 degrees C. The higher temperature hastens and stabilizes the diphenylamine reaction with DNA so that absorbances of samples can be measured as early as 3 h after the reaction is started. This shortened assay is able to detect as little as 3 micrograms of calf thymus DNA. This method is suitable for measuring DNA content of epidermal tissue.

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Year:  1988        PMID： 3189801     DOI： 10.1016/0003-2697(88)90156-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  13 in total

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Authors:  J D Singer; B M Manning; T Formosa
Journal:  Mol Cell Biol       Date:  1996-04       Impact factor: 4.272

2.  Transgenic grain legumes obtained by in planta electroporation-mediated gene transfer.

Authors:  G M Chowrira; V Akella; P E Fuerst; P F Lurquin
Journal:  Mol Biotechnol       Date:  1996-04       Impact factor: 2.695

3.  Acinetobacter calcoaceticus liberates chromosomal DNA during induction of competence by cell lysis.

Authors:  R Palmen; K J Hellingwerf
Journal:  Curr Microbiol       Date:  1995-01       Impact factor: 2.188

4.  Plasminogen deficiency leads to impaired lobular reorganization and matrix accumulation after chronic liver injury.

Authors:  J F Pohl; H Melin-Aldana; G Sabla; J L Degen; J A Bezerra
Journal:  Am J Pathol       Date:  2001-12       Impact factor: 4.307

5.  Unmodified prolactin (PRL) promotes PRL secretion and acidophil hypertrophy and is associated with pituitary hyperplasia in female rats.

Authors:  Terence E Johnson; Mayza Vue; Sharyn Brekhus; Amy Khong; Timothy W C Ho; Ameae M Walker
Journal:  Endocrine       Date:  2003 Feb-Mar       Impact factor: 3.633

6.  The colorimetric determination of selectively cleaved adenosines and guanosines in DNA oligomers using bicinchoninic acid and copper.

Authors:  Elizabeth M Thomas; Stephen M Testa
Journal:  J Biol Inorg Chem       Date:  2016-11-02       Impact factor: 3.358

7.  Genome-based metabolic mapping and 13C flux analysis reveal systematic properties of an oleaginous microalga Chlorella protothecoides.

Authors:  Chao Wu; Wei Xiong; Junbiao Dai; Qingyu Wu
Journal:  Plant Physiol       Date:  2014-12-15       Impact factor: 8.340

8.  Characterization of quail intestinal mucin as a ligand for endogenous quail lectin.

Authors:  R Fang; M Mantle; H Ceri
Journal:  Biochem J       Date:  1993-08-01       Impact factor: 3.857

9.  Role of nitric oxide and reactive oxygen species in static magnetic field pre-treatment induced tolerance to ambient UV-B stress in soybean.

Authors:  Sunita Kataria; Anshu Rastogi; Ankita Bele; Meeta Jain
Journal:  Physiol Mol Biol Plants       Date:  2020-04-20

10.  The role of cdc2 and other genes in meiosis in Schizosaccharomyces pombe.

Authors:  Y Iino; Y Hiramine; M Yamamoto
Journal:  Genetics       Date:  1995-08       Impact factor: 4.562
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