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Literature DB >> 7764230

Repeat units from a maize rDNA external spacer region exhibit DNA curvature and interact with high-mobility-group proteins.

Abstract

The 3-kb external spacer from a maize (Zea mays L. cv. A619) nuclear rRNA gene unit which contains nine highly homologous 200-bp repeat elements was found to include a region with DNA-curvature properties. The centre of curvature was localized within repeats 5 and 6 using a circular permutation assay. A 60-bp-long subfragment of this region was found to interact with nuclear proteins, including high-mobility-group (HMG) proteins, and with the maize HMGa protein synthesized in Escherichia coli from a recombinant plasmid. The potential influence of the binding of the HMG proteins on the conformation of this subfragment was studied with a permutation assay based on a bending vector.

Entities: Gene Species

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Year: 1993 PMID： 7764230 DOI： 10.1007/BF00195754

Source DB: PubMed Journal: Planta ISSN： 0032-0935 Impact factor: 4.116

48 in total

10. Molecular analysis of the Escherichia coli hns gene encoding a DNA-binding protein, which preferentially recognizes curved DNA sequences.

Authors: H Yamada; T Yoshida; K Tanaka; C Sasakawa; T Mizuno
Journal: Mol Gen Genet Date: 1991-11

5 in total

1. Interaction of nuclear proteins with intrinsically curved DNA in a matrix attachment region of a tobacco gene.

Authors: Y Fukuda
Journal: Plant Mol Biol Date: 2000-09 Impact factor: 4.076

2. An intrinsically bent region upstream of the transcription start site of the rRNA genes of Arabidopsis thaliana interacts with an HMG-related protein.

Authors: C Kneidl; E Dinkl; F Grummt
Journal: Plant Mol Biol Date: 1995-02 Impact factor: 4.076

3. Stability of the maize chromosomal high-mobility-group proteins, HMGa and HMGb, in vivo.

Authors: K D Grasser; W Hetz; G Feix
Journal: Plant Mol Biol Date: 1994-06 Impact factor: 4.076

4. Comparative analysis of chromosomal HMG proteins from monocotyledons and dicotyledons.

Authors: K D Grasser; T Wohlfarth; H Bäumlein; G Feix
Journal: Plant Mol Biol Date: 1993-11 Impact factor: 4.076

5. Ti plasmid-encoded octopine and nopaline catabolism in Agrobacterium: specificities of the LysR-type regulators OccR and NocR, and protein-induced DNA bending.

Authors: D Kreusch; J von Lintig; J Schröder
Journal: Mol Gen Genet Date: 1995-11-01

5 in total

Repeat units from a maize rDNA external spacer region exhibit DNA curvature and interact with high-mobility-group proteins.

1. Isolation and characterization of maize cDNAs encoding a high mobility group protein displaying a HMG-box.

2. Characterization of inherent curvature in DNA lacking polyadenine runs.

3. A non-curved chicken lysozyme 5' matrix attachment site is 3' followed by a strongly curved DNA sequence.

4. Occurrence of 9 homologous repeat units in the external spacer region of a nuclear maize rRNA gene unit.

5. Curved helix segments can uniquely orient the topology of supertwisted DNA.

6. Specific binding of nuclear proteins to the promoter region of a maize nuclear rRNA gene unit.

7. DNA bending and binding factors of the human beta-actin promoter.

8. A transcription factor, TFIS, interacts with both the promoter and enhancer of the Xenopus rRNA genes.