Literature DB >> 7763728

Cloning and sequence analysis of the cyclomaltodextrinase gene from Bacillus sphaericus and expression in Escherichia coli cells.

T Oguma1, A Matsuyama, M Kikuchi, E Nakano.   

Abstract

The gene for cyclomaltodextrinase (CDase; EC 3.2.1.54) from Bacillus sphaericus E-244 was cloned in the recombinant plasmid pCD629. Sequencing a portion of pCD629 revealed a unique open reading frame of 1,773 nucleotides coding for a 591-amino-acid polypeptide. The deduced polypeptide sequence showed about 50% homology with that of a neopullulanase, and was slightly homologous to those of the cyclodextrin glucanotransferases and the alpha-amylases. The optimum pH, specific activity and Km value for beta-cyclodextrin of the CDase that has been produced in Escherichia coli cells were 8.0, 16.4 units/mg protein, and 0.41 mM, respectively. These values were almost identical to those from B. sphaericus E-244.

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Year:  1993        PMID： 7763728     DOI： 10.1007/BF00228606

Source DB:  PubMed          Journal:  Appl Microbiol Biotechnol        ISSN： 0175-7598            Impact factor:   4.813


  21 in total

1.  Nucleotide sequence of the gene that encodes a neopullulanase from an alkalophilic Bacillus.

Authors:  K Igarashi; K Ara; K Saeki; K Ozaki; S Kawai; S Ito
Journal:  Biosci Biotechnol Biochem       Date:  1992-03       Impact factor: 2.043

2.  PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT.

Authors:  H SAITO; K I MIURA
Journal:  Biochim Biophys Acta       Date:  1963-08-20

3.  Purification and some properties of cyclodextrin-hydrolyzing enzyme from Bacillus sphaericus.

Authors:  T Oguma; M Kikuchi; K Mizusawa
Journal:  Biochim Biophys Acta       Date:  1990-10-12

4.  Nucleotide sequence of the Bacillus stearothermophilus alpha-amylase gene.

Authors:  R Nakajima; T Imanaka; S Aiba
Journal:  J Bacteriol       Date:  1985-07       Impact factor: 3.490

5.  Molecular cloning and nucleotide sequence of the cyclomaltodextrin glucanotransferase gene from the alkalophilic Bacillus sp. strain no. 38-2.

Authors:  T Kaneko; T Hamamoto; K Horikoshi
Journal:  J Gen Microbiol       Date:  1988-01

6.  Characterization of thermostable cyclodextrinase from Clostridium thermohydrosulfuricum 39E.

Authors:  B C Saha; J G Zeikus
Journal:  Appl Environ Microbiol       Date:  1990-09       Impact factor: 4.792

7.  Alpha-amylase genes (amyR2 and amyE+) from an alpha-amylase-hyperproducing Bacillus subtilis strain: molecular cloning and nucleotide sequences.

Authors:  H Yamazaki; K Ohmura; A Nakayama; Y Takeichi; K Otozai; M Yamasaki; G Tamura; K Yamane
Journal:  J Bacteriol       Date:  1983-10       Impact factor: 3.490

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  Glutamine phosphoribosylpyrophosphate amidotransferase from cloned Escherichia coli purF. NH2-terminal amino acid sequence, identification of the glutamine site, and trace metal analysis.

Authors:  J Y Tso; M A Hermodson; H Zalkin
Journal:  J Biol Chem       Date:  1982-04-10       Impact factor: 5.157

10.  Penicillinase from Bacillus licheniformis: nucleotide sequence of the gene and implications for the biosynthesis of a secretory protein in a Gram-positive bacterium.

Authors:  K Neugebauer; R Sprengel; H Schaller
Journal:  Nucleic Acids Res       Date:  1981-06-11       Impact factor: 16.971
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  6 in total

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Authors:  B Svensson
Journal:  Plant Mol Biol       Date:  1994-05       Impact factor: 4.076

2.  A new thermoactive pullulanase from Desulfurococcus mucosus: cloning, sequencing, purification, and characterization of the recombinant enzyme after expression in Bacillus subtilis.

Authors:  F Duffner; C Bertoldo; J T Andersen; K Wagner; G Antranikian
Journal:  J Bacteriol       Date:  2000-11       Impact factor: 3.490

3.  Extracellular synthesis, specific recognition, and intracellular degradation of cyclomaltodextrins by the hyperthermophilic archaeon Thermococcus sp. strain B1001.

Authors:  Y Hashimoto; T Yamamoto; S Fujiwara; M Takagi; T Imanaka
Journal:  J Bacteriol       Date:  2001-09       Impact factor: 3.490

4.  Structural elucidation of dextran degradation mechanism by streptococcus mutans dextranase belonging to glycoside hydrolase family 66.

Authors:  Nobuhiro Suzuki; Young-Min Kim; Zui Fujimoto; Mitsuru Momma; Masayuki Okuyama; Haruhide Mori; Kazumi Funane; Atsuo Kimura
Journal:  J Biol Chem       Date:  2012-02-15       Impact factor: 5.157

5.  Modes of action of acarbose hydrolysis and transglycosylation catalyzed by a thermostable maltogenic amylase, the gene for which was cloned from a Thermus strain.

Authors:  T J Kim; M J Kim; B C Kim; J C Kim; T K Cheong; J W Kim; K H Park
Journal:  Appl Environ Microbiol       Date:  1999-04       Impact factor: 4.792

6.  Improvement of cyclodextrin glycosyltransferase gene expression in Escherichia coli by insertion of regulatory sequences involved in the promotion of RNA transcription.

Authors:  Norhayati Ramli; Suraini Abd-Aziz; Noorjahan Banu Alitheen; Mohd Ali Hassan; Toshinari Maeda
Journal:  Mol Biotechnol       Date:  2013-07       Impact factor: 2.695
  6 in total

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