Literature DB >> 2275540

Characterization of thermostable cyclodextrinase from Clostridium thermohydrosulfuricum 39E.

B C Saha1, J G Zeikus.   

Abstract

Clostridium thermohydrosulfuricum 39E produced a cell-bound cyclodextrin (CD)-degrading enzyme (cyclodextrinase). It was partially purified 205-fold (specific activity, 14.5 U/mg of protein) by solubilizing with Triton X-100, ammonium sulfate treatment, and DEAE-Sepharose CL-6B column chromatography. The enzyme activity was found to be stable at pH 5.5 and 60 degrees C and optimally active at pH 6.0 and 65 degrees C. The enzyme preparation hydrolyzed CDs, with alpha-CD greater than beta-CD greater than gamma-CD, and displayed a putative multiple attack pattern. The enzyme activity was inhibited by p-chloromercuribenzoate but not by N-bromosuccinimide.

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Year:  1990        PMID： 2275540      PMCID： PMC184873          DOI： 10.1128/aem.56.9.2941-2943.1990

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

1.  A Pseudomonas intracellular amylase with high activity on maltodextrins and cyclodextrins.

Authors:  K Kato; T Sugimoto; A Amemura; T Harada
Journal:  Biochim Biophys Acta       Date:  1975-05-23

2.  Purification and Characterization of Extracellular Amylolytic Enzymes from the Yeast Filobasidium capsuligenum.

Authors:  R De Mot; H Verachtert
Journal:  Appl Environ Microbiol       Date:  1985-12       Impact factor: 4.792

3.  Purification and characterization of a novel thermostable beta-amylase from Clostridium thermosulphurogenes.

Authors:  G J Shen; B C Saha; Y E Lee; L Bhatnagar; J G Zeikus
Journal:  Biochem J       Date:  1988-09-15       Impact factor: 3.857

4.  Secretion of alpha-amylase and multiple forms of glucoamylase by the yeast Trichosporon pullulans.

Authors:  R De Mot; H Verachtert
Journal:  Can J Microbiol       Date:  1986-01       Impact factor: 2.419

5.  Properties of the raw-starch digesting amylase of Aspergillus sp. K-27: a synergistic action of glucoamylase and alpha-amylase.

Authors:  J I Abe; K Nakajima; H Nagano; S Hizukuri; K Obata
Journal:  Carbohydr Res       Date:  1988-04-01       Impact factor: 2.104

6.  Purification and characterization of a highly thermostable novel pullulanase from Clostridium thermohydrosulfuricum.

Authors:  B C Saha; S P Mathupala; J G Zeikus
Journal:  Biochem J       Date:  1988-06-01       Impact factor: 3.857

7.  Kinetic difference between hydrolyses of gamma-cyclodextrin by human salivary and pancreatic alpha-amylases.

Authors:  J J Marshall; I Miwa
Journal:  Biochim Biophys Acta       Date:  1981-09-15

8.  Substrate competition and specificity at the active site of amylopullulanase from Clostridium thermohydrosulfuricum.

Authors:  S Mathupala; B C Saha; J G Zeikus
Journal:  Biochem Biophys Res Commun       Date:  1990-01-15       Impact factor: 3.575

9.  Regulation and genetic enhancement of glucoamylase and pullulanase production in Clostridium thermohydrosulfuricum.

Authors:  H H Hyun; J G Zeikus
Journal:  J Bacteriol       Date:  1985-12       Impact factor: 3.490

10.  A bacterial glucoamylase degrading cyclodextrins. Partial purification and properties of the enzyme from a Flavobacterium species.

Authors:  H Bender
Journal:  Eur J Biochem       Date:  1981-04
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  9 in total

1.  Structure of the gene encoding cyclomaltodextrinase from Clostridium thermohydrosulfuricum 39E and characterization of the enzyme purified from Escherichia coli.

Authors:  S M Podkovyrov; J G Zeikus
Journal:  J Bacteriol       Date:  1992-08       Impact factor: 3.490

2.  Purification and characterization of periplasmic alpha-amylase from Xanthomonas campestris K-11151.

Authors:  J Abe; N Onitsuka; T Nakano; Y Shibata; S Hizukuri; E Entani
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490

Review 3.  Biology, ecology, and biotechnological applications of anaerobic bacteria adapted to environmental stresses in temperature, pH, salinity, or substrates.

Authors:  S E Lowe; M K Jain; J G Zeikus
Journal:  Microbiol Rev       Date:  1993-06

4.  Extracellular synthesis, specific recognition, and intracellular degradation of cyclomaltodextrins by the hyperthermophilic archaeon Thermococcus sp. strain B1001.

Authors:  Y Hashimoto; T Yamamoto; S Fujiwara; M Takagi; T Imanaka
Journal:  J Bacteriol       Date:  2001-09       Impact factor: 3.490

5.  Investigating the role of carbohydrate-binding module 34 in cyclomaltodextrinase from Geobacillus thermopakistaniensis: structural and functional analyses.

Authors:  Iqra Aroob; Maryam Javed; Nasir Ahmad; Mehwish Aslam; Naeem Rashid
Journal:  3 Biotech       Date:  2021-12-23       Impact factor: 2.406

6.  Cloning and sequence analysis of the cyclomaltodextrinase gene from Bacillus sphaericus and expression in Escherichia coli cells.

Authors:  T Oguma; A Matsuyama; M Kikuchi; E Nakano
Journal:  Appl Microbiol Biotechnol       Date:  1993-05       Impact factor: 4.813

7.  Metabolism of cyclodextrins by Klebsiella oxytoca m5a1: purification and characterisation of a cytoplasmically located cyclodextrinase.

Authors:  R Feederle; M Pajatsch; E Kremmer; A Böck
Journal:  Arch Microbiol       Date:  1996-03       Impact factor: 2.552

8.  β-cyclodextrin production by the cyclodextrin glucanotransferase from Paenibacillus illinoisensis ZY-08: cloning, purification, and properties.

Authors:  Yong-Suk Lee; Yi Zhou; Dong-Ju Park; Jie Chang; Yong-Lark Choi
Journal:  World J Microbiol Biotechnol       Date:  2012-12-23       Impact factor: 3.312

9.  Influence of Culture Conditions on the Bioreduction of Organic Acids to Alcohols by Thermoanaerobacter pseudoethanolicus.

Authors:  Sean Michael Scully; Aaron E Brown; Yannick Mueller-Hilger; Andrew B Ross; Jóhann Örlygsson
Journal:  Microorganisms       Date:  2021-01-12
  9 in total

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