Literature DB >> 7751391

Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples.

S N Cho1, G M van der Vliet, S Park, S H Baik, S K Kim, Y Chong, A H Kolk, P R Klatser, J D Kim.   

Abstract

We developed a colorimetric microwell plate hybridization assay (CoMPHA) for the specific detection of 5'-biotinylated amplified Mycobacterium tuberculosis DNA. The optical densities of the CoMPHA corresponded to the initial amounts of purified template DNA. Here, we show that the CoMPHA is useful in distinguishing the PCR-positive and PCR-negative samples.

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Year:  1995        PMID： 7751391      PMCID： PMC228029          DOI： 10.1128/jcm.33.3.752-754.1995

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  Polymerase chain reaction amplification of a repetitive DNA sequence specific for Mycobacterium tuberculosis.

Authors:  K D Eisenach; M D Cave; J H Bates; J T Crawford
Journal:  J Infect Dis       Date:  1990-05       Impact factor: 5.226

2.  Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions.

Authors:  M C Longo; M S Berninger; J L Hartley
Journal:  Gene       Date:  1990-09-01       Impact factor: 3.688

3.  IS6110: conservation of sequence in the Mycobacterium tuberculosis complex and its utilization in DNA fingerprinting.

Authors:  M D Cave; K D Eisenach; P F McDermott; J H Bates; J T Crawford
Journal:  Mol Cell Probes       Date:  1991-02       Impact factor: 2.365

4.  Detection and identification of Mycobacterium tuberculosis by DNA amplification.

Authors:  C C Pao; T S Yen; J B You; J S Maa; E H Fiss; C H Chang
Journal:  J Clin Microbiol       Date:  1990-09       Impact factor: 5.948

5.  Diagnosis of tuberculosis by DNA amplification in clinical practice evaluation.

Authors:  A Brisson-Noel; C Aznar; C Chureau; S Nguyen; C Pierre; M Bartoli; R Bonete; G Pialoux; B Gicquel; G Garrigue
Journal:  Lancet       Date:  1991-08-10       Impact factor: 79.321

6.  A more reliable PCR for detection of Mycobacterium tuberculosis in clinical samples.

Authors:  L F Kox; D Rhienthong; A M Miranda; N Udomsantisuk; K Ellis; J van Leeuwen; S van Heusden; S Kuijper; A H Kolk
Journal:  J Clin Microbiol       Date:  1994-03       Impact factor: 5.948

7.  Comparison of various repetitive DNA elements as genetic markers for strain differentiation and epidemiology of Mycobacterium tuberculosis.

Authors:  D van Soolingen; P E de Haas; P W Hermans; P M Groenen; J D van Embden
Journal:  J Clin Microbiol       Date:  1993-08       Impact factor: 5.948

8.  Progress toward a simplified polymerase chain reaction and its application to diagnosis of tuberculosis.

Authors:  S M Wilson; R McNerney; P M Nye; P D Godfrey-Faussett; N G Stoker; A Voller
Journal:  J Clin Microbiol       Date:  1993-04       Impact factor: 5.948

9.  Detection of Mycobacterium tuberculosis in clinical samples by using polymerase chain reaction and a nonradioactive detection system.

Authors:  A H Kolk; A R Schuitema; S Kuijper; J van Leeuwen; P W Hermans; J D van Embden; R A Hartskeerl
Journal:  J Clin Microbiol       Date:  1992-10       Impact factor: 5.948

10.  Direct detection of Mycobacterium tuberculosis in respiratory specimens in a clinical laboratory by polymerase chain reaction.

Authors:  B A Forbes; K E Hicks
Journal:  J Clin Microbiol       Date:  1993-07       Impact factor: 5.948
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  6 in total

1.  Evaluation of a novel PCR-based diagnostic assay for detection of Mycobacterium tuberculosis in sputum samples.

Authors:  M Maher; M Glennon; G Martinazzo; E Turchetti; S Marcolini; T Smith; M T Dawson
Journal:  J Clin Microbiol       Date:  1996-09       Impact factor: 5.948

2.  Comparative evaluation of two commercial amplification assays for direct detection of Mycobacterium tuberculosis complex in respiratory specimens.

Authors:  C Piersimoni; A Callegaro; D Nista; S Bornigia; F De Conti; G Santini; G De Sio
Journal:  J Clin Microbiol       Date:  1997-01       Impact factor: 5.948

3.  Microwell hybridization assay for detection of PCR products from Mycobacterium tuberculosis complex and the recombinant Mycobacterium smegmatis strain 1008 used as an internal control.

Authors:  L F Kox; G T Noordhoek; M Kunakorn; S Mulder; M Sterrenburg; A H Kolk
Journal:  J Clin Microbiol       Date:  1996-09       Impact factor: 5.948

4.  Evaluation of performances of three DNA enzyme immunoassays for detection of Helicobacter pylori PCR products from biopsy specimens.

Authors:  L Monteiro; J Cabrita; F Mégraud
Journal:  J Clin Microbiol       Date:  1997-11       Impact factor: 5.948

5.  Diagnostic value of PCR in genitourinary tuberculosis.

Authors:  Narotam Sharma; Veena Sharma; Prem Raj Singh; Shivani Sailwal; Rajeev S Kushwaha; Rajesh K Singh; Satish C Nautiyal; Pankaj Mishra; Tariq Masood; R K Singh
Journal:  Indian J Clin Biochem       Date:  2012-12-06

6.  Detection of point mutations associated with resistance of Helicobacter pylori to clarithromycin by hybridization in liquid phase.

Authors:  M Pina; A Occhialini; L Monteiro; H P Doermann; F Mégraud
Journal:  J Clin Microbiol       Date:  1998-11       Impact factor: 5.948
  6 in total

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