Literature DB >> 7751276

Identification of Lrp-regulated genes by inverse PCR and sequencing: regulation of two mal operons of Escherichia coli by leucine-responsive regulatory protein.

E Tchetina1, E B Newman.   

Abstract

We have used the technique of inverse PCR to identify Escherichia coli chromosomal genes carrying Lrp-regulated inserts. This technique revealed that malT, malEFG, and malB-lamB-malK are all activated two- to fivefold by Lrp and confirmed that Lrp regulates expression of the leuDBCA and livHJKG operons. lacZ transcription is also increased in the presence of Lrp. However, the growth rate of the Lrp mutant on maltose and lactose is not decreased by Lrp deficiency.

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Year:  1995        PMID： 7751276      PMCID： PMC176937          DOI： 10.1128/jb.177.10.2679-2683.1995

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  23 in total

1.  Dominant constitutive mutations in malT, the positive regulator gene of the maltose regulon in Escherichia coli.

Authors:  M Débarbouillé; H A Shuman; T J Silhavy; M Schwartz
Journal:  J Mol Biol       Date:  1978-09-15       Impact factor: 5.469

Review 2.  Linkage map of Escherichia coli K-12, edition 8.

Authors:  B J Bachmann
Journal:  Microbiol Rev       Date:  1990-06

3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  L-serine degradation in Escherichia coli K-12: cloning and sequencing of the sdaA gene.

Authors:  H S Su; B F Lang; E B Newman
Journal:  J Bacteriol       Date:  1989-09       Impact factor: 3.490

5.  Genetic applications of an inverse polymerase chain reaction.

Authors:  H Ochman; A S Gerber; D L Hartl
Journal:  Genetics       Date:  1988-11       Impact factor: 4.562

6.  The use of thin acrylamide gels for DNA sequencing.

Authors:  F Sanger; A R Coulson
Journal:  FEBS Lett       Date:  1978-03-01       Impact factor: 4.124

7.  The leucine regulon of Escherichia coli K-12: a mutation in rblA alters expression of L-leucine-dependent metabolic operons.

Authors:  L R Tuan; R D'Ari; E B Newman
Journal:  J Bacteriol       Date:  1990-08       Impact factor: 3.490

8.  A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli.

Authors:  M Singer; T A Baker; G Schnitzler; S M Deischel; M Goel; W Dove; K J Jaacks; A D Grossman; J W Erickson; C A Gross
Journal:  Microbiol Rev       Date:  1989-03

9.  Altered amino acid metabolism in lrp mutants of Escherichia coli K12 and their derivatives.

Authors:  G Ambartsoumian; R D'Ari; R T Lin; E B Newman
Journal:  Microbiology       Date:  1994-07       Impact factor: 2.777

10.  Expression of malT, the regulator gene of the maltose region in Escherichia coli, is limited both at transcription and translation.

Authors:  C Chapon
Journal:  EMBO J       Date:  1982       Impact factor: 11.598
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  12 in total

1.  The deoxycytidine pathway for thymidylate synthesis in Escherichia coli.

Authors:  Bernard Weiss
Journal:  J Bacteriol       Date:  2007-09-07       Impact factor: 3.490

2.  Production of 3-nitrosoindole derivatives by Escherichia coli during anaerobic growth.

Authors:  Young-Man Kwon; Bernard Weiss
Journal:  J Bacteriol       Date:  2009-06-26       Impact factor: 3.490

3.  Comparison of the sensitivities of two Escherichia coli genes to in vivo variation of Lrp concentration.

Authors:  C Chen; E B Newman
Journal:  J Bacteriol       Date:  1998-02       Impact factor: 3.490

4.  An Escherichia coli mutant that makes exceptionally long cells.

Authors:  Ziad W El-Hajj; Elaine B Newman
Journal:  J Bacteriol       Date:  2015-02-17       Impact factor: 3.490

5.  Influence of amino acids on low-density Escherichia coli responses to nutrient downshifts.

Authors:  Sergio Bodini; Luisa Nunziangeli; Francesca Santori
Journal:  J Bacteriol       Date:  2007-02-09       Impact factor: 3.490

6.  Use of an inducible regulatory protein to identify members of a regulon: application to the regulon controlled by the leucine-responsive regulatory protein (Lrp) in Escherichia coli.

Authors:  S P Bhagwat; M R Rice; R G Matthews; R M Blumenthal
Journal:  J Bacteriol       Date:  1997-10       Impact factor: 3.490

Review 7.  Maltose/maltodextrin system of Escherichia coli: transport, metabolism, and regulation.

Authors:  W Boos; H Shuman
Journal:  Microbiol Mol Biol Rev       Date:  1998-03       Impact factor: 11.056

8.  Escherichia coli Lrp (leucine-responsive regulatory protein) does not directly regulate expression of the leu operon promoter.

Authors:  J R Landgraf; J A Boxer; J M Calvo
Journal:  J Bacteriol       Date:  1999-10       Impact factor: 3.490

9.  Use of an in vivo titration method to study a global regulator: effect of varying Lrp levels on expression of gltBDF in Escherichia coli.

Authors:  D W Borst; R M Blumenthal; R G Matthews
Journal:  J Bacteriol       Date:  1996-12       Impact factor: 3.490

10.  Natural transformation of Campylobacter jejuni requires components of a type II secretion system.

Authors:  Rebecca S Wiesner; David R Hendrixson; Victor J DiRita
Journal:  J Bacteriol       Date:  2003-09       Impact factor: 3.490
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