Literature DB >> 7747977

Secretion of biologically active murine interleukin-2 by Lactococcus lactis subsp. lactis.

L Steidler1, J M Wells, A Raeymaekers, J Vandekerckhove, W Fiers, E Remaut.   

Abstract

Secretion of functional recombinant murine interleukin-2 (mIL2) by Lactococcus lactis was achieved by fusion of the sequence encoding mature mIL2 to the secretion signal leader of the lactococcal usp45 gene placed under transcriptional control of the phage T7 promoter-T7 RNA polymerase expression system. The recombinant mature mIL2 was one of only a few proteins which accumulated in the growth medium. Sequence analysis revealed correct processing at the first amino acid of the mature protein. A T-cell proliferation assay showed that the recombinant protein has the same specific biological activity as mIL2 obtained from a natural source.

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Year:  1995        PMID： 7747977      PMCID： PMC167420          DOI： 10.1128/aem.61.4.1627-1629.1995

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

1.  Cloning of usp45, a gene encoding a secreted protein from Lactococcus lactis subsp. lactis MG1363.

Authors:  M van Asseldonk; G Rutten; M Oteman; R J Siezen; W M de Vos; G Simons
Journal:  Gene       Date:  1990-10-30       Impact factor: 3.688

2.  Selective in vitro growth of T lymphocytes from normal human bone marrows.

Authors:  D A Morgan; F W Ruscetti; R Gallo
Journal:  Science       Date:  1976-09-10       Impact factor: 47.728

3.  Unique structure of murine interleukin-2 as deduced from cloned cDNAs.

Authors:  N Kashima; C Nishi-Takaoka; T Fujita; S Taki; G Yamada; J Hamuro; T Taniguchi
Journal:  Nature       Date:  1985 Jan 31-Feb 6       Impact factor: 49.962

Review 4.  T-T cell interactions during cytotoxic T lymphocyte (CTL) responses: T cell derived helper factor (Interleukin 2) as a probe to analyze CTL responsiveness and thymic maturation of CTL progenitors.

Authors:  H Wagner; C Hardt; K Heeg; K Pfizenmaier; W Solbach; R Bartlett; H Stockinger; M Röllinghoff
Journal:  Immunol Rev       Date:  1980       Impact factor: 12.988

5.  Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.

Authors:  M J Casadaban; S N Cohen
Journal:  J Mol Biol       Date:  1980-04       Impact factor: 5.469

6.  Efficient oligonucleotide-directed construction of mutations in expression vectors by the gapped duplex DNA method using alternating selectable markers.

Authors:  P Stanssens; C Opsomer; Y M McKeown; W Kramer; M Zabeau; H J Fritz
Journal:  Nucleic Acids Res       Date:  1989-06-26       Impact factor: 16.971

7.  Interleukin 2 induces antigen-reactive T cell lines to secrete BCGF-I.

Authors:  M Howard; L Matis; T R Malek; E Shevach; W Kell; D Cohen; K Nakanishi; W E Paul
Journal:  J Exp Med       Date:  1983-12-01       Impact factor: 14.307

8.  A model system for the investigation of heterologous protein secretion pathways in Lactococcus lactis.

Authors:  J M Wells; P W Wilson; P M Norton; R W Le Page
Journal:  Appl Environ Microbiol       Date:  1993-11       Impact factor: 4.792

9.  Species-specificity of T cell stimulating activities of IL 2 and BSF-1 (IL 4): comparison of normal and recombinant, mouse and human IL 2 and BSF-1 (IL 4).

Authors:  T R Mosmann; T Yokota; R Kastelein; S M Zurawski; N Arai; Y Takebe
Journal:  J Immunol       Date:  1987-03-15       Impact factor: 5.422

10.  Construction of a lactococcal expression vector: expression of hen egg white lysozyme in Lactococcus lactis subsp. lactis.

Authors:  M van de Guchte; J M van der Vossen; J Kok; G Venema
Journal:  Appl Environ Microbiol       Date:  1989-01       Impact factor: 4.792
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  17 in total

1.  Surface expression of the conserved C repeat region of streptococcal M6 protein within the Pip bacteriophage receptor of Lactococcus lactis.

Authors:  B L Geller; N Wade; T D Gilberts; D E Hruby; R Johanson; L Topisirovic
Journal:  Appl Environ Microbiol       Date:  2001-12       Impact factor: 4.792

2.  Expression of the Staphylococcus hyicus lipase in Lactococcus lactis.

Authors:  S Drouault; G Corthier; S D Ehrlich; P Renault
Journal:  Appl Environ Microbiol       Date:  2000-02       Impact factor: 4.792

3.  Nisin-controlled extracellular production of interleukin-2 in Lactococcus lactis strains, without the requirement for a signal peptide sequence.

Authors:  Antonio Fernández; Juan M Rodríguez; Roy J Bongaerts; Michael J Gasson; Nikki Horn
Journal:  Appl Environ Microbiol       Date:  2007-09-28       Impact factor: 4.792

4.  Oral delivery of glucagon like peptide-1 by a recombinant Lactococcus lactis.

Authors:  Payal Agarwal; Pulkit Khatri; Blasé Billack; Woon-Kai Low; Jun Shao
Journal:  Pharm Res       Date:  2014-06-14       Impact factor: 4.200

5.  Functional display of a heterologous protein on the surface of Lactococcus lactis by means of the cell wall anchor of Staphylococcus aureus protein A.

Authors:  L Steidler; J Viaene; W Fiers; E Remaut
Journal:  Appl Environ Microbiol       Date:  1998-01       Impact factor: 4.792

6.  A nine-residue synthetic propeptide enhances secretion efficiency of heterologous proteins in Lactococcus lactis.

Authors:  Y Le Loir; A Gruss; S D Ehrlich; P Langella
Journal:  J Bacteriol       Date:  1998-04       Impact factor: 3.490

7.  The peptidyl-prolyl isomerase motif is lacking in PmpA, the PrsA-like protein involved in the secretion machinery of Lactococcus lactis.

Authors:  Sophie Drouault; Jamila Anba; Sophie Bonneau; Alexander Bolotin; S Dusko Ehrlich; Pierre Renault
Journal:  Appl Environ Microbiol       Date:  2002-08       Impact factor: 4.792

8.  Application of the extracellular alpha-amylase gene from Streptococcus bovis 148 to construction of a secretion vector for yogurt starter strains.

Authors:  E Satoh; Y Ito; Y Sasaki; T Sasaki
Journal:  Appl Environ Microbiol       Date:  1997-11       Impact factor: 4.792

9.  Agrobacterium bioassay strain for ultrasensitive detection of N-acylhomoserine lactone-type quorum-sensing molecules: detection of autoinducers in Mesorhizobium huakuii.

Authors:  Jun Zhu; Yunrong Chai; Zengtao Zhong; Shunpeng Li; Stephen C Winans
Journal:  Appl Environ Microbiol       Date:  2003-11       Impact factor: 4.792

Review 10.  Inducible gene expression systems in Lactococcus lactis.

Authors:  G M Djordjevic; T R Klaenhammer
Journal:  Mol Biotechnol       Date:  1998-04       Impact factor: 2.695
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