| Literature DB >> 7723629 |
A E Kingsbury1, O J Foster, A P Nisbet, N Cairns, L Bray, D J Eve, A J Lees, C D Marsden.
Abstract
The relationship between pH and mRNA preservation in post-mortem human brain was examined using in situ hybridization histochemistry and Northern hybridization with oligonucleotide probes in a large group of human subjects, including control and neuropathological cases. Tissue pH was found to correlate strongly with preservation of four mRNA species in three brain areas. Tissue with low pH, assumed to result from prolonged terminal hypoxia, contained reduced or absent mRNA, while tissue with higher pH was found to contain quantifiable amounts, the values for pathological brain samples being comparable to those for control material of similar pH. Measurement of tissue pH provides a simple means to screen post-mortem brain for mRNA preservation and is suggested as a means to match material in case-control studies of human neurodegenerative disease.Entities:
Mesh：
Substances：
Year: 1995 PMID： 7723629 DOI： 10.1016/0169-328x(94)00219-5
Source DB: PubMed Journal: Brain Res Mol Brain Res ISSN： 0169-328X