Literature DB >> 769785

Conversion of pepsinogen into pepsin is not a one-step process.

C W Dykes, J Kay.   

Abstract

By incubation of pepsinogen with pepstatin at pH2.5, the first 'active' protein generated on activation is trapped in an inactive complex. The first activation peptide liberated has been identified as residues 1-16 from the pepsinogen sequence. This suggests a sequential mechanism rather than a one-step formation of pepsin.

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Year:  1976        PMID： 769785      PMCID： PMC1172551          DOI： 10.1042/bj1530141

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  7 in total

1.  An assay to differentiate the "true" activity of pepsin from the potential activity of pepsinogen.

Authors:  J Kay
Journal:  Anal Biochem       Date:  1975-08       Impact factor: 3.365

2.  The activation of bovine pepsinogen. Sequence of the peptides released, identification of a pepsin inhibitor.

Authors:  M Harboe; P M Andersen; B Foltmann; J Kay; B Kassell
Journal:  J Biol Chem       Date:  1974-07-25       Impact factor: 5.157

Review 3.  Zymogens of proteolytic enzymes.

Authors:  B Kassell; J Kay
Journal:  Science       Date:  1973-06-08       Impact factor: 47.728

4.  N-terminal sequence of swine pepsinogen and pepsin. The site of pepsinogen activation.

Authors:  V M Stepanov; L A Baratova; I B Pugacheva; L P Belyanova; L P Revina; E A Timokhina
Journal:  Biochem Biophys Res Commun       Date:  1973-10-01       Impact factor: 3.575

5.  Kinetics and mechanism of pepsinogen activation.

Authors:  J al-Janabi; J A Hartsuck; J Tang
Journal:  J Biol Chem       Date:  1972-07-25       Impact factor: 5.157

6.  Primary structure of porcine pepsin. III. Amino acid sequence of a cyanogen bromide fragment, CB2A, and the complete structure of porcine pepsin.

Authors:  P Sepulveda; J Marciniszyn; D Liu; J Tang
Journal:  J Biol Chem       Date:  1975-07-10       Impact factor: 5.157

7.  Effect of pepstatin on acid proteases.

Authors:  T Aoyagi; S Kunimoto; H Morishima; T Takeuchi; H Umezawa
Journal:  J Antibiot (Tokyo)       Date:  1971-10       Impact factor: 2.649
  7 in total
  6 in total

1.  Isolation of procathepsin D from mature cathepsin D by pepstatin affinity chromatography. Autocatalytic proteolysis of the zymogen form of the enzyme.

Authors:  G E Conner
Journal:  Biochem J       Date:  1989-10-15       Impact factor: 3.857

2.  Purification and characterization of pepsinogens and pepsins from the stomach of rice field eel (Monopterus albus Zuiew).

Authors:  Wu-Yin Weng; Tao Wu; Wei-Qin Chen; Guang-Ming Liu; Kiyoshi Osatomi; Wen-Jin Su; Min-Jie Cao
Journal:  Fish Physiol Biochem       Date:  2010-12-08       Impact factor: 2.794

3.  Interaction of pepstatin with pig pepsinogen.

Authors:  J Kay; C W Dykes
Journal:  Biochem J       Date:  1976-08-01       Impact factor: 3.857

Review 4.  Mechanism of activation of the gastric aspartic proteinases: pepsinogen, progastricsin and prochymosin.

Authors:  C Richter; T Tanaka; R Y Yada
Journal:  Biochem J       Date:  1998-11-01       Impact factor: 3.857

5.  Conformational instability of the N- and C-terminal lobes of porcine pepsin in neutral and alkaline solutions.

Authors:  X Lin; J A Loy; F Sussman; J Tang
Journal:  Protein Sci       Date:  1993-09       Impact factor: 6.725

6.  The first step in the activation of chicken pepsinogen is similar to that of prochymosin.

Authors:  H Keilova; V Kostka; J Kay
Journal:  Biochem J       Date:  1977-12-01       Impact factor: 3.857
  6 in total

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