Literature DB >> 7695304

PCR mapping of integrons reveals several novel combinations of resistance genes.

C Lévesque1, L Piché, C Larose, P H Roy.   

Abstract

The integron is a new type of mobile element which has evolved by a site-specific recombinational mechanism. Integrons consist of two conserved segments of DNA separated by a variable region containing one or more genes integrated as cassettes. Oligonucleotide probes specific for the conserved segments have revealed that integrons are widespread in recently isolated clinical bacteria. Also, by using oligonucleotide probes for several antibiotic resistance genes, we have found novel combinations of resistance genes in these strains. By using PCR, we have determined the content and order of the resistance genes inserted between the conserved segments in the integrons of these clinical isolates. PCR mapping of integrons can be a useful epidemiological tool to study the evolution of multiresistance plasmids and transposons and dissemination of antibiotic resistance genes.

Entities:  

Mesh：

Substances：

Year:  1995        PMID： 7695304      PMCID： PMC162507          DOI： 10.1128/AAC.39.1.185

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  43 in total

1.  Complete nucleotide sequence of the Escherichia coli plasmid pBR322.

Authors:  J G Sutcliffe
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1979

2.  Revised analysis of aadA2 gene of plasmid pSa.

Authors:  A Bito; M Susani
Journal:  Antimicrob Agents Chemother       Date:  1994-05       Impact factor: 5.191

3.  Nucleotide sequence of the Tn10 encoded tetracycline resistance gene.

Authors:  W Hillen; K Schollmeier
Journal:  Nucleic Acids Res       Date:  1983-01-25       Impact factor: 16.971

4.  Genes for gentamicin-(3)-N-acetyltransferases III and IV: I. Nucleotide sequence of the AAC(3)-IV gene and possible involvement of an IS140 element in its expression.

Authors:  B Bräu; U Pilz; W Piepersberg
Journal:  Mol Gen Genet       Date:  1984

5.  Transposable plasmid deoxyribonucleic acid sequence in Pseudomonas aeruginosa which mediates resistance to gentamicin and four other antimicrobial agents.

Authors:  C E Rubens; W F McNeill; W E Farrar
Journal:  J Bacteriol       Date:  1979-09       Impact factor: 3.490

6.  Identification of the type I trimethoprim-resistant dihydrofolate reductase specified by the Escherichia coli R-plasmid R483: comparison with procaryotic and eucaryotic dihydrofolate reductases.

Authors:  C C Simonsen; E Y Chen; A D Levinson
Journal:  J Bacteriol       Date:  1983-09       Impact factor: 3.490

7.  Cross infection in a surgical ward caused by Pseudomonas aeruginosa with transferable resistance to gentamicin and tobramycin.

Authors:  F R Falkiner; C T Keane; M Dalton; M T Clancy; G A Jacoby
Journal:  J Clin Pathol       Date:  1977-08       Impact factor: 3.411

8.  A transposon, Tn732, encoding gentamicin/tobramycin resistance.

Authors:  M E Nugent; D H Bone; N Datta
Journal:  Nature       Date:  1979-11-22       Impact factor: 49.962

9.  Amikacin resistance mediated by multiresistance transposon Tn2424.

Authors:  J F Meyer; B A Nies; B Wiedemann
Journal:  J Bacteriol       Date:  1983-08       Impact factor: 3.490

10.  Physical and functional mapping of Tn2603, a transposon encoding ampicillin, streptomycin, sulfonamide, and mercury resistance.

Authors:  T Yamamoto; M Tanaka; R Baba; S Yamagishi
Journal:  Mol Gen Genet       Date:  1981
View more
  324 in total

1.  Multidrug resistance is mediated by large plasmids carrying a class 1 integron in the emergent Salmonella enterica serotype [4,5,12:i:-].

Authors:  B Guerra; S M Soto; J M Argüelles; M C Mendoza
Journal:  Antimicrob Agents Chemother       Date:  2001-04       Impact factor: 5.191

2.  Outbreak of infections caused by Pseudomonas aeruginosa producing VIM-1 carbapenemase in Greece.

Authors:  A Tsakris; S Pournaras; N Woodford; M F Palepou; G S Babini; J Douboyas; D M Livermore
Journal:  J Clin Microbiol       Date:  2000-03       Impact factor: 5.948

3.  Antimicrobial resistance and spread of class 1 integrons among Salmonella serotypes.

Authors:  B Guerra; S Soto; S Cal; M C Mendoza
Journal:  Antimicrob Agents Chemother       Date:  2000-08       Impact factor: 5.191

4.  Integrons and gene cassettes in the enterobacteriaceae.

Authors:  P A White; C J McIver; W D Rawlinson
Journal:  Antimicrob Agents Chemother       Date:  2001-09       Impact factor: 5.191

5.  Novel gene cassettes and integrons.

Authors:  E D Peters; M A Leverstein-van Hall; A T Box; J Verhoef; A C Fluit
Journal:  Antimicrob Agents Chemother       Date:  2001-10       Impact factor: 5.191

6.  Characterization of plasmids carrying CMY-2 from expanded-spectrum cephalosporin-resistant Salmonella strains isolated in the United States between 1996 and 1998.

Authors:  A Carattoli; F Tosini; W P Giles; M E Rupp; S H Hinrichs; F J Angulo; T J Barrett; P D Fey
Journal:  Antimicrob Agents Chemother       Date:  2002-05       Impact factor: 5.191

7.  Increased prevalence of class I integrons in Escherichia coli, Klebsiella species, and Enterobacter species isolates over a 7-year period in a German university hospital.

Authors:  F J Schmitz; D Hafner; R Geisel; P Follmann; C Kirschke; J Verhoef; K Köhrer; A C Fluit
Journal:  J Clin Microbiol       Date:  2001-10       Impact factor: 5.948

8.  Detection of a variant metallo-beta-lactamase, IMP-10, from two unrelated strains of Pseudomonas aeruginosa and an alcaligenes xylosoxidans strain.

Authors:  Shizuko Iyobe; Haruko Kusadokoro; Ayako Takahashi; Sachie Yomoda; Toyoji Okubo; Akio Nakamura; Koji O'Hara
Journal:  Antimicrob Agents Chemother       Date:  2002-06       Impact factor: 5.191

9.  Excision and integration of cassettes by an integron integrase of Nitrosomonas europaea.

Authors:  Grégory Léon; Paul H Roy
Journal:  J Bacteriol       Date:  2003-03       Impact factor: 3.490

10.  Novel variant (bla(VIM-4)) of the metallo-beta-lactamase gene bla(VIM-1) in a clinical strain of Pseudomonas aeruginosa.

Authors:  Spyros Pournaras; Athanassios Tsakris; Maria Maniati; Leonidas S Tzouvelekis; Antonios N Maniatis
Journal:  Antimicrob Agents Chemother       Date:  2002-12       Impact factor: 5.191
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.