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Literature DB >> 7695273

Characterization of the PNT1 pentamidine resistance gene of Saccharomyces cerevisiae.

Abstract

The Saccharomyces cerevisiae PNT1 gene was isolated and characterized. When present in high copy number in S. cerevisiae, PNT1 confers resistance to the anti-Pneumocystis carinii drug pentamidine. The PNT1 gene encodes a previously uncharacterized polypeptide of 409 amino acids. The predicted gene product is a very basic (pI 9.9) polypeptide with one potential membrane-associated region. PNT1 is located on chromosome XVR of S. cerevisiae. It is transcribed at a very low level. Overexpression of the gene increases resistance to the cytostatic and mitochondrial DNA-damaging effects of pentamidine and related cationic compounds. Disruption of the gene leads to slightly increased levels of susceptibility to pentamidine and some related compounds.

Entities: Chemical Gene Species

Mesh：

Substances：
Antimycin A
Pentamidine

Year: 1994 PMID： 7695273 PMCID： PMC188296 DOI： 10.1128/AAC.38.12.2850

Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN： 0066-4804 Impact factor: 5.191

26 in total

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5 in total

1. In vitro activities of pentamidine, pyrimethamine, trimethoprim, and sulfonamides against Aspergillus species.

Authors: Javier Afeltra; Jacques F G M Meis; Roxana G Vitale; Johan W Mouton; Paul E Verweij
Journal: Antimicrob Agents Chemother Date: 2002-06 Impact factor: 5.191

2. Mutations affecting a yeast mitochondrial inner membrane protein, pnt1p, block export of a mitochondrially synthesized fusion protein from the matrix.

Authors: S He; T D Fox
Journal: Mol Cell Biol Date: 1999-10 Impact factor: 4.272