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Literature DB >> 7680121

Replacement of RNA hairpins by in vitro selected tetranucleotides.

B Dichtl¹, T Pan, A B DiRenzo, O C Uhlenbeck.

Abstract

An in vitro selection method based on the autolytic cleavage of yeast tRNA(Phe) by Pb2+ was applied to obtain tRNA derivatives with the anticodon hairpin replaced by four single-stranded nucleotides. Based on the rates of the site-specific cleavage by Pb2+ and the presence of a specific UV-induced crosslink, certain tetranucleotide sequences allow proper folding of the rest of the tRNA molecule, whereas others do not. One such successful tetramer sequence was also used to replace the acceptor stem of yeast tRNA(Phe) and the anticodon hairpin of E.coli tRNA(Phe) without disrupting folding. These experiments suggest that certain tetramers may be able to replace structurally nonessential hairpins in any RNA.

Entities: Chemical Species

Mesh：

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Year: 1993 PMID： 7680121 PMCID： PMC309149 DOI： 10.1093/nar/21.3.531

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

19 in total

1. An ultraviolet light-induced crosslink in yeast tRNA(Phe).

Authors: L S Behlen; J R Sampson; O C Uhlenbeck
Journal: Nucleic Acids Res Date: 1992-08-11 Impact factor: 16.971

2. Miniribozymes, small derivatives of the sunY intron, are catalytically active.

Authors: J A Doudna; J W Szostak
Journal: Mol Cell Biol Date: 1989-12 Impact factor: 4.272

3. Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase.

Authors: J R Sampson; A B DiRenzo; L S Behlen; O C Uhlenbeck
Journal: Science Date: 1989-03-10 Impact factor: 47.728

4. Specific cleavages of pure tRNAs by plumbous ions.

Authors: C Werner; B Krebs; G Keith; G Dirheimer
Journal: Biochim Biophys Acta Date: 1976-05-03

5. The design and catalytic properties of a simplified ribonuclease P RNA.

Authors: D S Waugh; C J Green; N R Pace
Journal: Science Date: 1989-06-30 Impact factor: 47.728

6. Characterization of the lead(II)-induced cleavages in tRNAs in solution and effect of the Y-base removal in yeast tRNAPhe.

Authors: W J Krzyzosiak; T Marciniec; M Wiewiorowski; P Romby; J P Ebel; R Giegé
Journal: Biochemistry Date: 1988-07-26 Impact factor: 3.162

7. Deletion of nonconserved helices near the 3' end of the rRNA intron of Tetrahymena thermophila alters self-splicing but not core catalytic activity.

Authors: E T Barfod; T R Cech
Journal: Genes Dev Date: 1988-06 Impact factor: 11.361

Replacement of RNA hairpins by in vitro selected tetranucleotides.

1. An ultraviolet light-induced crosslink in yeast tRNA(Phe).

2. Miniribozymes, small derivatives of the sunY intron, are catalytically active.

3. Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase.

4. Specific cleavages of pure tRNAs by plumbous ions.

5. The design and catalytic properties of a simplified ribonuclease P RNA.

6. Characterization of the lead(II)-induced cleavages in tRNAs in solution and effect of the Y-base removal in yeast tRNAPhe.

7. Deletion of nonconserved helices near the 3' end of the rRNA intron of Tetrahymena thermophila alters self-splicing but not core catalytic activity.

8. Characterization of RNA hairpin loop stability.

Review 9. Comparative anatomy of 16-S-like ribosomal RNA.

10. Crystallographic and biochemical investigation of the lead(II)-catalyzed hydrolysis of yeast phenylalanine tRNA.

1. Kinetic characterization of intramolecular and intermolecular hammerhead RNAs with stem II deletions.

2. Crystallization of ribozymes and small RNA motifs by a sparse matrix approach.

3. Frequent use of the same tertiary motif by self-folding RNAs.

4. Selection for active E. coli tRNA(Phe) variants from a randomized library using two proteins.