Literature DB >> 7678193

Fine mapping of HIV-1 Nef-epitopes by monoclonal antibodies.

H Siakkou1, S Jahn, N Kienzle, R Ulrich, C Grötzinger, T Schneider, B Kohleisen, G Pauli, R Spohn, G Jung.   

Abstract

A panel of newly isolated murine monoclonal antibodies is described which are specific for the Nef protein of the human immunodeficiency virus type 1 (HIV-1). Epitope mapping using recombinant Nef-related proteins, synthetic peptides and lipopeptides showed 3 independent antigenic determinants located within the regions of amino acids 83-93, 175-190 and 86-166 of the Nef protein. None of the monoclonal antibodies reacted with recombinant Nef proteins of HIV-2.

Entities:  

Mesh：

Substances：

Year:  1993        PMID： 7678193     DOI： 10.1007/bf01309790

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  44 in total

1.  A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.

Authors:  J F Kearney; A Radbruch; B Liesegang; K Rajewsky
Journal:  J Immunol       Date:  1979-10       Impact factor: 5.422

2.  Semiautomated T-bag peptide synthesis using 9-fluorenyl-methoxycarbonyl strategy and benzotriazol-1-yl-tetramethyl-uronium tetrafluoroborate activation.

Authors:  A G Beck-Sickinger; H Dürr; G Jung
Journal:  Pept Res       Date:  1991 Mar-Apr

3.  Defined monoclonal antibodies to Escherichia coli beta-galactosidase as a tool for characterisation of recombinant expression products.

Authors:  M Bröker; H P Harthus
Journal:  FEBS Lett       Date:  1989-10-23       Impact factor: 4.124

4.  Human immunodeficiency virus type 1 negative factor is a transcriptional silencer.

Authors:  T M Niederman; B J Thielan; L Ratner
Journal:  Proc Natl Acad Sci U S A       Date:  1989-02       Impact factor: 11.205

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Production and characterization of monoclonal antibodies against human Cu/Zn superoxide dismutase and the establishment of a super-rapid enzyme-linked immunosorbent assay (SURALISA).

Authors:  T Porstmann; R Wietschke; R Grunow; S Jahn; B Porstmann; H Schmechta; M Pergande; R Von Baehr
Journal:  J Immunol Methods       Date:  1990-02-20       Impact factor: 2.303

7.  Epitopes of the HIV-1-negative factor (nef) reactive with murine monoclonal antibodies and human HIV-1-positive sera.

Authors:  T Schneider; H P Harthus; P Hildebrandt; M Niedrig; M Bröker; W Weigelt; A Beck; G Pauli
Journal:  AIDS Res Hum Retroviruses       Date:  1991-01       Impact factor: 2.205

8.  Analysis of adenovirus transforming proteins from early regions 1A and 1B with antisera to inducible fusion antigens produced in Escherichia coli.

Authors:  K R Spindler; D S Rosser; A J Berk
Journal:  J Virol       Date:  1984-01       Impact factor: 5.103

9.  Cellular localization of Nef expressed in persistently HIV-1-infected low-producer astrocytes.

Authors:  B Kohleisen; M Neumann; R Herrmann; R Brack-Werner; K J Krohn; V Ovod; A Ranki; V Erfle
Journal:  AIDS       Date:  1992-12       Impact factor: 4.177

10.  Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins.

Authors:  K K Stanley; J P Luzio
Journal:  EMBO J       Date:  1984-06       Impact factor: 11.598
View more
  3 in total

1.  Recognition of prominent viral epitopes induced by immunization with human immunodeficiency virus type 1 regulatory genes.

Authors:  J Hinkula; C Svanholm; S Schwartz; P Lundholm; M Brytting; G Engström; R Benthin; H Glaser; G Sutter; B Kohleisen; V Erfle; K Okuda; H Wigzell; B Wahren
Journal:  J Virol       Date:  1997-07       Impact factor: 5.103

2.  Hamster polyomavirus-encoded proteins: gene cloning, heterologous expression and immunoreactivity.

Authors:  R Ulrich; K Sommerfeld; A Schröder; H Prokoph; W Arnold; D H Krüger; S Scherneck
Journal:  Virus Genes       Date:  1996       Impact factor: 2.332

3.  Subcloning of HIV-2 nef genes in E. coli and immunological reactivity of expressed fusion proteins.

Authors:  R Ulrich; H Siakkou; J Mayer; N Kienzle; N Müller-Lantzsch; D H Krüger
Journal:  Virus Genes       Date:  1993-09       Impact factor: 2.332
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.