| Literature DB >> 8279123 |
R Ulrich1, H Siakkou, J Mayer, N Kienzle, N Müller-Lantzsch, D H Krüger.
Abstract
The nef gene located in the 3' region of the HIV-2 genome encodes an N-terminally myristylated protein of 27-35 kD, likely to be involved in the regulation of viral transcription. The nef genes of HIV-2 isolates GH-1, ROD, ST, BEN, and D194.17 were inserted into E. coli pEX vectors and expression of Nef beta-galactosidase fusion proteins was detected in stained gels. All fusion proteins specifically reacted with a rabbit serum raised against bacterially expressed Nef from HIV-2D194.17. Sera from monkeys inoculated with HIV-2BEN or SIVMAC251 recognized the Nef proteins of only certain HIV-2 isolates. No cross-reactivity of these sera with HIV-1 Nef and of a rabbit anti-HIV-1-Nef serum with the described HIV-2 Nef fusion proteins was observed.Entities:
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Year: 1993 PMID： 8279123 DOI： 10.1007/bf01702584
Source DB: PubMed Journal: Virus Genes ISSN： 0920-8569 Impact factor: 2.332