Literature DB >> 7666559

Binding kinetics of ecotropic (Moloney) murine leukemia retrovirus with NIH 3T3 cells.

H Yu1, N Soong, W F Anderson.   

Abstract

A quantitative analysis of the binding kinetics of intact Moloney murine leukemia retrovirus (MoMuLV) particles with NIH 3T3 cells was performed with an immunofluorescence flow cytometry assay. The virus-cell binding equilibrium dissociation constant (KD), expressed in terms of virus particle concentration, was measured to be 8.5 (+/- 6.4) x 10(-12) M at 4 degrees C and was three- to sixfold lower at temperatures above 15 degrees C. The KD of virus binding is about 1,000-fold lower than the KD of purified MoMuLV envelope. The association rate constant was determined to be 2.5 (+/- 0.9) x 10(9) M-1 min-1 at 4 degrees C and was 5- to 10-fold higher at temperatures above 15 degrees C. The apparent dissociation rate constant at 4 degrees C was 1.1 (+/- 0.4) x 10(-3) min-1 and was doubled for every 10 degrees C increase in temperature over the range tested (15 to 37 degrees C).

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Year:  1995        PMID： 7666559      PMCID： PMC189559     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  35 in total

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Authors:  M Strand; J T August
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Authors:  L A Lasky; G Nakamura; D H Smith; C Fennie; C Shimasaki; E Patzer; P Berman; T Gregory; D J Capon
Journal:  Cell       Date:  1987-09-11       Impact factor: 41.582

Review 3.  Diffusion-controlled macromolecular interactions.

Authors:  O G Berg; P H von Hippel
Journal:  Annu Rev Biophys Biophys Chem       Date:  1985

4.  Role of diffusion in ligand binding to macromolecules and cell-bound receptors.

Authors:  D Shoup; A Szabo
Journal:  Biophys J       Date:  1982-10       Impact factor: 4.033

5.  Characterization of Rauscher murine leukemia virus envelope glycoprotein receptor in membranes from murine fibroblasts.

Authors:  V S Kalyanaraman; M G Sarngadharan; R C Gallo
Journal:  J Virol       Date:  1978-12       Impact factor: 5.103

6.  Analysis of the interaction between Rauscher murine leukemia virus and murine cell membrane receptor by in vitro binding assay.

Authors:  K Ganguly; V S Kalyanaraman; M G Sarngadharan
Journal:  Cancer Lett       Date:  1983-02       Impact factor: 8.679

7.  A safe packaging line for gene transfer: separating viral genes on two different plasmids.

Authors:  D Markowitz; S Goff; A Bank
Journal:  J Virol       Date:  1988-04       Impact factor: 5.103

8.  In vitro mutagenesis identifies a region within the envelope gene of the human immunodeficiency virus that is critical for infectivity.

Authors:  R L Willey; D H Smith; L A Lasky; T S Theodore; P L Earl; B Moss; D J Capon; M A Martin
Journal:  J Virol       Date:  1988-01       Impact factor: 5.103

9.  Lymphoid cell surface receptor for Moloney leukemia virus envelope glycoprotein gp71. I. Binding characteristics.

Authors:  J Choppin; L Schaffar-Deshayes; P Debré; J P Lévy
Journal:  J Immunol       Date:  1981-06       Impact factor: 5.422

10.  The human immunodeficiency virus gp120 binding site on CD4: delineation by quantitative equilibrium and kinetic binding studies of mutants in conjunction with a high-resolution CD4 atomic structure.

Authors:  U Moebius; L K Clayton; S Abraham; S C Harrison; E L Reinherz
Journal:  J Exp Med       Date:  1992-08-01       Impact factor: 14.307
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  36 in total

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Authors:  M Chung; K Kizhatil; L M Albritton; G N Gaulton
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Journal:  J Virol       Date:  2001-04       Impact factor: 5.103

3.  Identification of the block in targeted retroviral-mediated gene transfer.

Authors:  Y Zhao; L Zhu; S Lee; L Li; E Chang; N W Soong; D Douer; W F Anderson
Journal:  Proc Natl Acad Sci U S A       Date:  1999-03-30       Impact factor: 11.205

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Authors:  E Bachrach; M Marin; M Pelegrin; G Karavanas; M Piechaczyk
Journal:  J Virol       Date:  2000-09       Impact factor: 5.103

5.  Determination of infectious retrovirus concentration from colony-forming assay with quantitative analysis.

Authors:  Young Jik Kwon; Gene Hung; W French Anderson; Ching-An Peng; Hong Yu
Journal:  J Virol       Date:  2003-05       Impact factor: 5.103

6.  Human immunodeficiency virus type 1 spinoculation enhances infection through virus binding.

Authors:  U O'Doherty; W J Swiggard; M H Malim
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7.  Targeted entry via somatostatin receptors using a novel modified retrovirus glycoprotein that delivers genes at levels comparable to those of wild-type viral glycoproteins.

Authors:  Fang Li; Byoung Y Ryu; Robin L Krueger; Scott A Heldt; Lorraine M Albritton
Journal:  J Virol       Date:  2011-10-19       Impact factor: 5.103

8.  Identification of envelope protein residues required for the expanded host range of 10A1 murine leukemia virus.

Authors:  J Y Han; P M Cannon; K M Lai; Y Zhao; M V Eiden; W F Anderson
Journal:  J Virol       Date:  1997-11       Impact factor: 5.103

9.  Synchronized infection of cell cultures by magnetically controlled virus.

Authors:  Hillel Haim; Israel Steiner; Amos Panet
Journal:  J Virol       Date:  2005-01       Impact factor: 5.103

10.  The amphotropic and ecotropic murine leukemia virus envelope TM subunits are equivalent mediators of direct membrane fusion: implications for the role of the ecotropic envelope and receptor in syncytium formation and viral entry.

Authors:  J A Ragheb; H Yu; T Hofmann; W F Anderson
Journal:  J Virol       Date:  1995-11       Impact factor: 5.103
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