Analysis of the interaction between Rauscher murine leukemia virus and murine cell membrane receptor by in vitro binding assay.

Binding of 125I-labeled gp70 of Rauscher murine leukemia virus (R-MuLV) by 3 murine cell lines, BALB/c-3T3, NIH/3T3 and KA-31 (Kirsten murine sarcoma virus transformed clone A-31 of BALB/c-3T3) cells was measured. The binding was a saturable process, dependent on the concentration of gp70 and on the number of cells. In no experiment could we demonstrate any quantitative utilization of gp70 in the medium. However, gp70 remaining in the spent medium could be bound to fresh cells in a subsequent incubation. BALB/c-3T3, NIH/3T3 and KA-31 cells showed similar association constants (1.2-2.5 x 10(8) M-1) for the binding. Moreover, all 3 cell lines had similar number of receptors (7.4-8.9 x 10(5)) per cell. Neither N- and B-tropism of the cells nor transformation by a sarcoma virus altered the number and type of the cell surface receptors.