Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 TAFs and TFIIA mediate differential utilization of the tandem Adh promoters.

Literature DB >> 7664336

TAFs and TFIIA mediate differential utilization of the tandem Adh promoters.

Abstract

The D. melanogaster alcohol dehydrogenase (Adh) gene is transcribed from two tandem promoters that are differentially utilized at various stages during development. To determine the mechanism of promoter selectivity, we have analyzed the activity of the Adh promoters both in vitro and in transfected cells. We found that selective promoter utilization is controlled by distinct initiator elements. Reconstitution of Adh transcription with purified components requires a specific TBP-TAF complex that, in concert with TFIIA, directs differential Adh promoter transcription. Fractionation of this TBP-TAF complex reveals that TAFII150 is required for discrimination between the proximal and distal promoters. We propose a mechanism for regulating differential promoter utilization during Drosophila development that involves the recognition of specific initiator elements by TAFs in the TFIID complex.

Entities: Gene Species

Mesh：

Substances：

Year: 1995 PMID： 7664336 DOI： 10.1016/0092-8674(95)90029-2

Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582

Keyword Cloud
Cited

41 in total

1. The downstream promoter element DPE appears to be as widely used as the TATA box in Drosophila core promoters.

Authors: A K Kutach; J T Kadonaga
Journal: Mol Cell Biol Date: 2000-07 Impact factor: 4.272

2. Characterization and functional analysis of Arabidopsis TFIIA reveal that the evolutionarily unconserved region of the large subunit has a transcription activation domain.

Authors: Y F Li; J Le Gourierrec; M Torki; Y J Kim; F Guerineau; D X Zhou
Journal: Plant Mol Biol Date: 1999-02 Impact factor: 4.076

3. The two Saccharomyces cerevisiae SUA7 (TFIIB) transcripts differ at the 3'-end and respond differently to stress.

Authors: B C Hoopes; G D Bowers; M J DiVisconte
Journal: Nucleic Acids Res Date: 2000-11-15 Impact factor: 16.971

4. Drosophila head segmentation factor buttonhead interacts with the same TATA box-binding protein-associated factors and in vivo DNA targets as human Sp1 but executes a different biological program.

Authors: F Schöck; F Sauer; H Jäckle; B A Purnell
Journal: Proc Natl Acad Sci U S A Date: 1999-04-27 Impact factor: 11.205

5. Functional interaction between TATA and upstream CACGTG elements regulates the temporally specific expression of Otx mRNAs during early embryogenesis of the sea urchin, Hemicentrotus pulcherrimus.

Authors: Akiko Kobayashi; Koji Akasaka; Masashi Kawaichi; Tetsuro Kokubo
Journal: Nucleic Acids Res Date: 2002-07-15 Impact factor: 16.971

TAFs and TFIIA mediate differential utilization of the tandem Adh promoters.

1. The downstream promoter element DPE appears to be as widely used as the TATA box in Drosophila core promoters.

2. Characterization and functional analysis of Arabidopsis TFIIA reveal that the evolutionarily unconserved region of the large subunit has a transcription activation domain.

3. The two Saccharomyces cerevisiae SUA7 (TFIIB) transcripts differ at the 3'-end and respond differently to stress.

4. Drosophila head segmentation factor buttonhead interacts with the same TATA box-binding protein-associated factors and in vivo DNA targets as human Sp1 but executes a different biological program.

5. Functional interaction between TATA and upstream CACGTG elements regulates the temporally specific expression of Otx mRNAs during early embryogenesis of the sea urchin, Hemicentrotus pulcherrimus.

Review 6. Multi-protein complexes in eukaryotic gene transcription.

7. Enhancer choice in cis and in trans in Drosophila melanogaster: role of the promoter.

8. ATG deserts define a novel core promoter subclass.

9. Core promoter elements can regulate transcription on a separate chromosome in trans.

10. Interaction of the viral activator protein ICP4 with TFIID through TAF250.