Literature DB >> 7656730

Identification of anticoagulant activities in the salivary glands of the soft tick, Ornithodoros savignyi.

A R Gaspar1, J C Crause, A W Neitz.   

Abstract

Salivary gland extracts of the sand tampan, Ornithodoros savignyi, prolonged the activated partial thromboplastin time (APTT) and prothrombin time (PT) significantly in a concentration-dependent manner. There was also a pronounced inhibition of human activated factor Xa (fXa) by salivary gland extracts. The salivary gland extracts inhibited chromogenic assays specific for both fXa and thrombin. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of the salivary gland proteins followed by elution of specific areas or bands from a polyvinylidene difluoride (PVDF)-membrane, showed that various anticoagulant factors are present when screened by means of the APTT assay. The most active component was associated with a band of M(r) of 14 kDa. Partial purification of this component was achieved using isoelectric focusing (IEF) and size-exclusion high-performance liquid chromatography (HPLC).

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Year:  1995        PMID： 7656730     DOI： 10.1007/bf00052551

Source DB:  PubMed          Journal:  Exp Appl Acarol        ISSN： 0168-8162            Impact factor:   2.132


  11 in total

1.  Purification and characterization of an anticoagulant from the salivary glands of the ixodid tick Rhipicephalus appendiculatus.

Authors:  M K Limo; W P Voigt; A G Tumbo-Oeri; R M Njogu; O K ole-MoiYoi
Journal:  Exp Parasitol       Date:  1991-05       Impact factor: 2.011

2.  Preparative elution of proteins blotted to Immobilon membranes.

Authors:  B Szewczyk; D F Summers
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3.  Production of high purity proteins by elution of individual fractions from two-dimensional gels.

Authors:  M N Tsvetkova; L I Kovalyov; A V Leptev; S S Shishkin
Journal:  Electrophoresis       Date:  1991 Jul-Aug       Impact factor: 3.535

4.  Anticoagulant in the tick Ixodes holocyclus.

Authors:  P Anastopoulos; M J Thurn; K W Broady
Journal:  Aust Vet J       Date:  1991-11       Impact factor: 1.281

5.  Tick anticoagulant peptide (TAP) is a novel inhibitor of blood coagulation factor Xa.

Authors:  L Waxman; D E Smith; K E Arcuri; G P Vlasuk
Journal:  Science       Date:  1990-05-04       Impact factor: 47.728

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 7.  Role of saliva in blood-feeding by arthropods.

Authors:  J M Ribeiro
Journal:  Annu Rev Entomol       Date:  1987       Impact factor: 19.686

8.  Inventory of coagulation inhibitors from animals feeding on blood. A report prepared on behalf of the Scientific and Standardization Committee's Registry of Exogenous Hemostatic Factors.

Authors:  F Markwardt
Journal:  Thromb Haemost       Date:  1994-09       Impact factor: 5.249

9.  Isolation and characterization of an anticoagulant present in the salivary glands of the bont-legged tick, Hyalomma truncatum.

Authors:  A M Joubert; J C Crause; A R Gaspar; F C Clarke; A M Spickett; A W Neitz
Journal:  Exp Appl Acarol       Date:  1995-02       Impact factor: 2.132

10.  Antihemostatic, antiinflammatory, and immunosuppressive properties of the saliva of a tick, Ixodes dammini.

Authors:  J M Ribeiro; G T Makoul; J Levine; D R Robinson; A Spielman
Journal:  J Exp Med       Date:  1985-02-01       Impact factor: 14.307
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  8 in total

1.  Apyrase activity and platelet aggregation inhibitors in the tick Ornithodoros savignyi (Acari: Argasidae).

Authors:  B J Mans; A R Gaspar; A I Louw; A W Neitz
Journal:  Exp Appl Acarol       Date:  1998-06       Impact factor: 2.132

2.  Cloning, nucleotide sequence and expression of the gene encoding factor Xa inhibitor from the salivary glands of the tick, Ornithodoros savignyi.

Authors:  A M Joubert; A I Louw; F Joubert; A W Neitz
Journal:  Exp Appl Acarol       Date:  1998-10       Impact factor: 2.132

3.  Thrombin inhibitor from the salivary gland of the camel tick Hyalomma dromedarii.

Authors:  Mahmoud A Ibrahim; Hassan M M Masoud
Journal:  Exp Appl Acarol       Date:  2017-12-19       Impact factor: 2.132

4.  Isolation and characterization of an anticoagulant from the salivary glands of the tick, Ornithodoros savignyi (Acari: Argasidae).

Authors:  A R Gaspar; A M Joubert; J C Crause; A W Neitz
Journal:  Exp Appl Acarol       Date:  1996-10       Impact factor: 2.132

5.  Isolation and properties of two forms of thrombin inhibitor from the nymphs of the camel tick Hyalomma dromedarii (Acari: Ixodidae).

Authors:  M A Ibrahim; A H Ghazy; T Maharem; M Khalil
Journal:  Exp Appl Acarol       Date:  2001       Impact factor: 2.132

6.  Characterization of anti-hemostatic factors in the argasid, Argas monolakensis: implications for the evolution of blood-feeding in the soft tick family.

Authors:  Ben J Mans; John F Andersen; Tom G Schwan; José M C Ribeiro
Journal:  Insect Biochem Mol Biol       Date:  2007-09-25       Impact factor: 4.714

Review 7.  The role of saliva in tick feeding.

Authors:  Ivo M B Francischetti; Anderson Sa-Nunes; Ben J Mans; Isabel M Santos; Jose M C Ribeiro
Journal:  Front Biosci (Landmark Ed)       Date:  2009-01-01

Review 8.  Quantitative Visions of Reality at the Tick-Host Interface: Biochemistry, Genomics, Proteomics, and Transcriptomics as Measures of Complete Inventories of the Tick Sialoverse.

Authors:  Ben J Mans
Journal:  Front Cell Infect Microbiol       Date:  2020-09-11       Impact factor: 5.293
  8 in total

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