| Literature DB >> 7646359 |
S Harpin1, S M Elahi, E Cornaglia, R H Yolken, Y Elazhary.
Abstract
The 5' untranslated region (UTR) of several bovine viral diarrhea virus (BVDV) isolates from the severe Quebec outbreak was amplified by polymerase chain reaction (PCR) and sequenced. Sequences revealed the loss, for the BVDV type II isolates, of an internal PstI restriction site, which is present in all known BVDV type 1 5' UTR sequences. A single restriction enzyme digestion (PstI) of an aliquot of PCR product allowed us to differentiate BVDV type I and BVDV type II.Entities:
Mesh:
Year: 1995 PMID: 7646359 DOI: 10.1007/bf01322754
Source DB: PubMed Journal: Arch Virol ISSN: 0304-8608 Impact factor: 2.574