Literature DB >> 762170

An electron microscopic study of pyruvate carboxylase.

N H Goss, P Y Dyer, D B Keech, J C Wallace.   

Abstract

Pyruvate carboxylases, purified to homogeneity from sheep and chicken liver, have been examined in the electron microscope and found to have a splayed tetrahedral structure which, in some views, appears similar to that described for this enzyme isolated from yeast. The square-planar tetrameric image previously ascribed to pyruvate carboxylases from vertebrate sources was shown to be that of a molecule which is not only larger than pyruvate carboxylase, but also devoid of biotin.

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Year:  1979        PMID： 762170

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  10 in total

1.  The existence of multiple tetrameric conformers of chicken liver pyruvate carboxylase and their roles in dilution inactivation.

Authors:  P V Attwood; W Johannssen; A Chapman-Smith; J C Wallace
Journal:  Biochem J       Date:  1993-03-01       Impact factor: 3.857

2.  The carboxybiotin complex of chicken liver pyruvate carboxylase. A kinetic analysis of the effects of acetyl-CoA, Mg2+ ions and temperature on its stability and on its reaction with 2-oxobutyrate.

Authors:  P V Attwood; J C Wallace
Journal:  Biochem J       Date:  1986-04-15       Impact factor: 3.857

3.  Further studies on the localization of the reactive lysyl residue of pyruvate carboxylase.

Authors:  A Chapman-Smith; G W Booker; P R Clements; J C Wallace; D B Keech
Journal:  Biochem J       Date:  1991-06-15       Impact factor: 3.857

4.  The carboxybiotin complex of pyruvate carboxylase. A kinetic analysis of the effects of Mg2+ ions on its stability and on its reaction with pyruvate.

Authors:  P V Attwood; J C Wallace; D B Keech
Journal:  Biochem J       Date:  1984-04-01       Impact factor: 3.857

5.  Inactivation of chicken liver pyruvate carboxylase by 1,10-phenanthroline.

Authors:  J A Carver; G S Baldwin; D B Keech; R Bais; J C Wallace
Journal:  Biochem J       Date:  1988-06-01       Impact factor: 3.857

6.  Cloning, sequencing and expression of rat liver pyruvate carboxylase.

Authors:  S Jitrapakdee; G W Booker; A I Cassady; J C Wallace
Journal:  Biochem J       Date:  1996-06-01       Impact factor: 3.857

7.  Factors that influence the translocation of the N-carboxybiotin moiety between the two sub-sites of pyruvate carboxylase.

Authors:  G J Goodall; G S Baldwin; J C Wallace; D B Keech
Journal:  Biochem J       Date:  1981-12-01       Impact factor: 3.857

8.  Bicarbonate-dependent ATP cleavage catalysed by pyruvate carboxylase in the absence of pyruvate.

Authors:  P V Attwood; B D Graneri
Journal:  Biochem J       Date:  1992-11-01       Impact factor: 3.857

9.  Rat liver pyruvate carboxylase. Purification, detection and quantification of apo and holo forms by immuno-blotting and by an enzyme-linked immunosorbent assay.

Authors:  F Ahmad; P M Ahmad; A Mendez
Journal:  Biochem J       Date:  1986-06-01       Impact factor: 3.857

10.  Pyruvate carboxylase catalysis of phosphate transfer between carbamoyl phosphate and ADP.

Authors:  P V Attwood; B D Graneri
Journal:  Biochem J       Date:  1991-01-15       Impact factor: 3.857
  10 in total

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