Literature DB >> 7615758

Differentiation of pathogenic Escherichia coli strains in Brazilian children by PCR.

N G Tornieporth1, J John, K Salgado, P de Jesus, E Latham, M C Melo, S T Gunzburg, L W Riley.   

Abstract

A PCR technique to differentiate pathogenic enteric Escherichia coli strains in a field setting was evaluated. Among 76 children with acute diarrhea, this technique identified 12 children (16%) with enterotoxigenic E. coli, 6 (8%) with enteropathogenic E. coli, and 1 (1%) with enteroinvasive E. coli infection. Compared with the conventional assays, the PCR method proved to be simpler, more rapid, and inexpensive and therefore suitable for application in a developing-country field setting.

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Year:  1995        PMID： 7615758      PMCID： PMC228169          DOI： 10.1128/jcm.33.5.1371-1374.1995

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

1.  Heat stable enterotoxin produced by Escherichia coli in acute diarrhoea.

Authors:  A Guarino; M Alessio; L Tarallo; M Fontana; G Iacono; L Gobio Casali; S Guandalini
Journal:  Arch Dis Child       Date:  1989-06       Impact factor: 3.791

2.  Identification of Escherichia coli that produces heat-stable enterotoxin STA by a commercially available enzyme-linked immunoassay and comparison of the assay with infant mouse and DNA probe tests.

Authors:  S M Scotland; G A Willshaw; B Said; H R Smith; B Rowe
Journal:  J Clin Microbiol       Date:  1989-07       Impact factor: 5.948

3.  Multi-gene amplification: simultaneous detection of three virulence genes in diarrhoeal stool.

Authors:  G Frankel; J A Giron; J Valmassoi; G K Schoolnik
Journal:  Mol Microbiol       Date:  1989-12       Impact factor: 3.501

4.  Identification of enteropathogenic Escherichia coli by PCR-based detection of the bundle-forming pilus gene.

Authors:  S T Gunzburg; N G Tornieporth; L W Riley
Journal:  J Clin Microbiol       Date:  1995-05       Impact factor: 5.948

5.  Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells.

Authors:  I C Scaletsky; M L Silva; L R Trabulsi
Journal:  Infect Immun       Date:  1984-08       Impact factor: 3.441

6.  Microtiter ganglioside enzyme-linked immunosorbent assay for vibrio and Escherichia coli heat-labile enterotoxins and antitoxin.

Authors:  D A Sack; S Huda; P K Neogi; R R Daniel; W M Spira
Journal:  J Clin Microbiol       Date:  1980-01       Impact factor: 5.948

7.  Enteropathogens associated with acute diarrheal disease in urban infants in São Paulo, Brazil.

Authors:  T A Gomes; V Rassi; K L MacDonald; S R Ramos; L R Trabulsi; M A Vieira; B E Guth; J A Candeias; C Ivey; M R Toledo
Journal:  J Infect Dis       Date:  1991-08       Impact factor: 5.226

8.  Use of Shigella flexneri ipaC and ipaH gene sequences for the general identification of Shigella spp. and enteroinvasive Escherichia coli.

Authors:  M M Venkatesan; J M Buysse; D J Kopecko
Journal:  J Clin Microbiol       Date:  1989-12       Impact factor: 5.948

9.  Primary structure of heat-labile enterotoxin produced by Escherichia coli pathogenic for humans.

Authors:  T Yamamoto; T Tamura; T Yokota
Journal:  J Biol Chem       Date:  1984-04-25       Impact factor: 5.157

Review 10.  Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent.

Authors:  M M Levine
Journal:  J Infect Dis       Date:  1987-03       Impact factor: 5.226
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  30 in total

1.  Use of deoxyribose by intestinal and extraintestinal pathogenic Escherichia coli strains: a metabolic adaptation involved in competitiveness.

Authors:  Christine Bernier-Fébreau; Laurence du Merle; Evelyne Turlin; Valérie Labas; Juana Ordonez; Anne-Marie Gilles; Chantal Le Bouguénec
Journal:  Infect Immun       Date:  2004-10       Impact factor: 3.441

2.  Prevalence of shiga toxin-producing Escherichia coli in a diarrheagenic Tunisian population, and the report of isolating STEC O157:H7 in Tunis.

Authors:  Nazek Al-Gallas; Olfa Bahri; Ridha Ben Aissa
Journal:  Curr Microbiol       Date:  2006-11-17       Impact factor: 2.188

3.  Environmental change and infectious disease: how new roads affect the transmission of diarrheal pathogens in rural Ecuador.

Authors:  Joseph N S Eisenberg; William Cevallos; Karina Ponce; Karen Levy; Sarah J Bates; James C Scott; Alan Hubbard; Nadia Vieira; Pablo Endara; Mauricio Espinel; Gabriel Trueba; Lee W Riley; James Trostle
Journal:  Proc Natl Acad Sci U S A       Date:  2006-12-07       Impact factor: 11.205

4.  Microbiological laboratory results from Haiti: June-October 1995.

Authors:  J J Drabick; J M Gambel; E Huck; S De Young; L Hardeman
Journal:  Bull World Health Organ       Date:  1997       Impact factor: 9.408

5.  Identifying etiological agents causing diarrhea in low income Ecuadorian communities.

Authors:  Gabriela Vasco; Gabriel Trueba; Richard Atherton; Manuel Calvopiña; William Cevallos; Thamara Andrade; Martha Eguiguren; Joseph N S Eisenberg
Journal:  Am J Trop Med Hyg       Date:  2014-07-21       Impact factor: 2.345

6.  Prevalence of diarrheagenic Escherichia coli in finns with or without diarrhea during a round-the-world trip.

Authors:  M Keskimäki; L Mattila; H Peltola; A Siitonen
Journal:  J Clin Microbiol       Date:  2000-12       Impact factor: 5.948

7.  Locals get travellers' diarrhoea too: risk factors for diarrhoeal illness and pathogenic Escherichia coli infection across an urban-rural gradient in Ecuador.

Authors:  Shanon M Smith; Lorena Montero; Maritza Paez; Estefania Ortega; Eric Hall; Kate Bohnert; Xavier Sanchez; Edison Puebla; Pablo Endara; William Cevallos; Gabriel Trueba; Karen Levy
Journal:  Trop Med Int Health       Date:  2018-12-06       Impact factor: 2.622

8.  Multiplex PCRs for identification of Escherichia coli virulence genes.

Authors:  M A Pass; R Odedra; R M Batt
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

9.  Identification of unconventional intestinal pathogenic Escherichia coli isolates expressing intermediate virulence factor profiles by using a novel single-step multiplex PCR.

Authors:  Daniel Müller; Lilo Greune; Gerhard Heusipp; Helge Karch; Angelika Fruth; Helmut Tschäpe; M Alexander Schmidt
Journal:  Appl Environ Microbiol       Date:  2007-03-30       Impact factor: 4.792

10.  Detection of Zoonotic Enteropathogens in Children and Domestic Animals in a Semirural Community in Ecuador.

Authors:  Karla Vasco; Jay P Graham; Gabriel Trueba
Journal:  Appl Environ Microbiol       Date:  2016-06-30       Impact factor: 4.792
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