Literature DB >> 10790141

Multiplex PCRs for identification of Escherichia coli virulence genes.

M A Pass1, R Odedra, R M Batt.   

Abstract

PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs.

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Year:  2000        PMID： 10790141      PMCID： PMC86652     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  Detection of various variant verotoxin genes in Escherichia coli by polymerase chain reaction.

Authors:  Z Lin; H Kurazono; S Yamasaki; Y Takeda
Journal:  Microbiol Immunol       Date:  1993       Impact factor: 1.955

2.  Isolation of Vero cytotoxin-producing Escherichia coli serotypes O9ab:H- and O101:H-carrying VT2 variant gene sequences from a patient with haemolytic uraemic syndrome.

Authors:  A Thomas; T Cheasty; H Chart; B Rowe
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1994-12       Impact factor: 3.267

3.  Presence of pap-, sfa-, and afa-related sequences in necrotoxigenic Escherichia coli isolates from cattle: evidence for new variants of the AFA family.

Authors:  J G Mainil; E Jacquemin; F Hérault; E Oswald
Journal:  Can J Vet Res       Date:  1997-07       Impact factor: 1.310

4.  Rapid and specific detection of verotoxin genes in Escherichia coli by the polymerase chain reaction.

Authors:  D R Pollard; W M Johnson; H Lior; S D Tyler; K R Rozee
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

5.  Detection of Shigella and enteroinvasive Escherichia coli by PCR in the stools of patients with dysentery in Thailand.

Authors:  O Sethabutr; P Echeverria; C W Hoge; L Bodhidatta; C Pitarangsi
Journal:  J Diarrhoeal Dis Res       Date:  1994-12

6.  Development of PCR for screening of enteroaggregative Escherichia coli.

Authors:  H Schmidt; C Knop; S Franke; S Aleksic; J Heesemann; H Karch
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948

7.  Identification of Shiga-like toxin type II producing Escherichia coli using the polymerase chain reaction and a digoxigenin labelled DNA probe.

Authors:  M P Jackson
Journal:  Mol Cell Probes       Date:  1992-06       Impact factor: 2.365

8.  Sequence heterogeneity of the eae gene and detection of verotoxin-producing Escherichia coli using serotype-specific primers.

Authors:  M Louie; J de Azavedo; R Clarke; A Borczyk; H Lior; M Richter; J Brunton
Journal:  Epidemiol Infect       Date:  1994-06       Impact factor: 2.451

9.  Detection of enterotoxigenic Escherichia coli after polymerase chain reaction amplification with a thermostable DNA polymerase.

Authors:  D M Olive
Journal:  J Clin Microbiol       Date:  1989-02       Impact factor: 5.948

10.  Detection of Escherichia coli and identification of enterotoxigenic strains by primer-directed enzymatic amplification of specific DNA sequences.

Authors:  U Candrian; B Furrer; C Höfelein; R Meyer; M Jermini; J Lüthy
Journal:  Int J Food Microbiol       Date:  1991-04       Impact factor: 5.277
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  41 in total

1.  Rapid detection of Shiga toxin-producing bacteria in feces by multiplex PCR with molecular beacons on the smart cycler.

Authors:  Simon D Bélanger; Maurice Boissinot; Christian Ménard; François J Picard; Michel G Bergeron
Journal:  J Clin Microbiol       Date:  2002-04       Impact factor: 5.948

2.  Multiplex PCR assay for the detection of five putative virulence genes encoded in verotoxigenic Escherichia coli plasmids.

Authors:  A V Bustamante; A M Sanso; P M A Lucchesi; A E Parma
Journal:  Curr Microbiol       Date:  2011-01-29       Impact factor: 2.188

3.  Identification of bacterial populations in dairy wastewaters by use of 16S rRNA gene sequences and other genetic markers.

Authors:  Jeffery A McGarvey; William G Miller; Susan Sanchez; Larry Stanker
Journal:  Appl Environ Microbiol       Date:  2004-07       Impact factor: 4.792

4.  Phylogenetic background of attaching and effacing Escherichia coli isolates from animals.

Authors:  C Tramuta; P Robino; P Nebbia
Journal:  Vet Res Commun       Date:  2008-05-29       Impact factor: 2.459

Review 5.  Epidemiology and clinical manifestations of enteroaggregative Escherichia coli.

Authors:  Betina Hebbelstrup Jensen; Katharina E P Olsen; Carsten Struve; Karen Angeliki Krogfelt; Andreas Munk Petersen
Journal:  Clin Microbiol Rev       Date:  2014-07       Impact factor: 26.132

6.  Unusual "flesh-eating" strains of Escherichia coli.

Authors:  Hila Shaked; Zmira Samra; Michal Paul; Liora Madar-Shapiro; Jonathan Cohen; Silvio Pitlik; Jihad Bishara
Journal:  J Clin Microbiol       Date:  2012-10-03       Impact factor: 5.948

7.  Pediatric norovirus diarrhea in Nicaragua.

Authors:  Filemon Bucardo; Johan Nordgren; Beatrice Carlsson; Margarita Paniagua; Per-Eric Lindgren; Felix Espinoza; Lennart Svensson
Journal:  J Clin Microbiol       Date:  2008-06-18       Impact factor: 5.948

8.  Evaluation of broiler litter with reference to the microbial composition as assessed by using 16S rRNA and functional gene markers.

Authors:  Jingrang Lu; Susan Sanchez; Charles Hofacre; John J Maurer; Barry G Harmon; Margie D Lee
Journal:  Appl Environ Microbiol       Date:  2003-02       Impact factor: 4.792

9.  A set of novel multiplex Taqman real-time PCRs for the detection of diarrhoeagenic Escherichia coli and its use in determining the prevalence of EPEC and EAEC in a university hospital.

Authors:  Christoph Hardegen; Sabine Messler; Birgit Henrich; Klaus Pfeffer; Jens Würthner; Colin R MacKenzie
Journal:  Ann Clin Microbiol Antimicrob       Date:  2010-01-22       Impact factor: 3.944

10.  Single multiplex polymerase chain reaction to detect diverse loci associated with diarrheagenic Escherichia coli.

Authors:  Catalina López-Saucedo; Jorge F Cerna; Nicolas Villegas-Sepulveda; Rocío Thompson; F Raul Velazquez; Javier Torres; Phillip I Tarr; Teresa Estrada-García
Journal:  Emerg Infect Dis       Date:  2003-01       Impact factor: 6.883
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