Literature DB >> 7615759

Identification of enteropathogenic Escherichia coli by PCR-based detection of the bundle-forming pilus gene.

S T Gunzburg1, N G Tornieporth, L W Riley.   

Abstract

A rapid and simple method of detecting enteropathogenic Escherichia coli (EPEC) was developed. The procedure is based on amplifying by the PCR method a 326-bp region of the bundle-forming pilus gene of EPEC. The oligonucleotide DNA primers used in this procedure did not amplify DNA of any other bacterial enteropathogens tested. The procedure was 100% specific for EPEC strains that exhibit a characteristic pattern of attachment (localized adherence) to HeLa cells.

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Year:  1995        PMID： 7615759      PMCID： PMC228170          DOI： 10.1128/jcm.33.5.1375-1377.1995

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

1.  Isolation and characterization of the localized adherence factor of enteropathogenic Escherichia coli.

Authors:  I C Scaletsky; S R Milani; L R Trabulsi; L R Travassos
Journal:  Infect Immun       Date:  1988-11       Impact factor: 3.441

2.  Plasmid-mediated adhesion in enteropathogenic Escherichia coli.

Authors:  M M Baldini; J B Kaper; M M Levine; D C Candy; H W Moon
Journal:  J Pediatr Gastroenterol Nutr       Date:  1983       Impact factor: 2.839

3.  Rapid and specific detection of verotoxin genes in Escherichia coli by the polymerase chain reaction.

Authors:  D R Pollard; W M Johnson; H Lior; S D Tyler; K R Rozee
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

4.  Screening for enteropathogenic Escherichia coli in infants with diarrhea by the fluorescent-actin staining test.

Authors:  S Knutton; A D Phillips; H R Smith; R J Gross; R Shaw; P Watson; E Price
Journal:  Infect Immun       Date:  1991-01       Impact factor: 3.441

5.  Detection of an adherence factor of enteropathogenic Escherichia coli with a DNA probe.

Authors:  J P Nataro; M M Baldini; J B Kaper; R E Black; N Bravo; M M Levine
Journal:  J Infect Dis       Date:  1985-09       Impact factor: 5.226

6.  Distribution of the bundle-forming pilus structural gene (bfpA) among enteropathogenic Escherichia coli.

Authors:  J A Girón; M S Donnenberg; W C Martin; K G Jarvis; J B Kaper
Journal:  J Infect Dis       Date:  1993-10       Impact factor: 5.226

7.  Enteropathogens associated with acute diarrheal disease in urban infants in São Paulo, Brazil.

Authors:  T A Gomes; V Rassi; K L MacDonald; S R Ramos; L R Trabulsi; M A Vieira; B E Guth; J A Candeias; C Ivey; M R Toledo
Journal:  J Infect Dis       Date:  1991-08       Impact factor: 5.226

8.  Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction.

Authors:  H Karch; T Meyer
Journal:  J Clin Microbiol       Date:  1989-12       Impact factor: 5.948

9.  Localized adherence by enteropathogenic Escherichia coli is an inducible phenotype associated with the expression of new outer membrane proteins.

Authors:  J Vuopio-Varkila; G K Schoolnik
Journal:  J Exp Med       Date:  1991-11-01       Impact factor: 14.307

Review 10.  Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent.

Authors:  M M Levine
Journal:  J Infect Dis       Date:  1987-03       Impact factor: 5.226
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  89 in total

1.  Expression of the bundle-forming pilus by enteropathogenic Escherichia coli strains of heterologous serotypes.

Authors:  Juana Gismero-Ordoñez; Monique Dall'Agnol; Luiz R Trabulsi; Jorge A Girón
Journal:  J Clin Microbiol       Date:  2002-06       Impact factor: 5.948

2.  Reduced etiological role for enteropathogenic Escherichia coli in cases of diarrhea in Brazilian infants.

Authors:  Josias Rodrigues; Cristiane M Thomazini; Alexandra Morelli; Georgea C M de Batista
Journal:  J Clin Microbiol       Date:  2004-01       Impact factor: 5.948

Review 3.  Virulence of enteropathogenic Escherichia coli, a global pathogen.

Authors:  S C Clarke; R D Haigh; P P E Freestone; P H Williams
Journal:  Clin Microbiol Rev       Date:  2003-07       Impact factor: 26.132

4.  Typing of intimin (eae) genes in attaching and effacing Escherichia coli strains from monkeys.

Authors:  Miguel Blanco; Jesús E Blanco; Jorge Blanco; Vânia Maria de Carvalho; Daniela Lopes Onuma; Antonio F Pestana de Castro
Journal:  J Clin Microbiol       Date:  2004-03       Impact factor: 5.948

5.  Epidemiological investigation of eaeA-positive Escherichia coli and Escherichia albertii strains isolated from healthy wild birds.

Authors:  Jae-Young Oh; Min-Su Kang; Hee-Tae Hwang; Byung-Ki An; Jun-Hun Kwon; Yong-Kuk Kwon
Journal:  J Microbiol       Date:  2011-11-09       Impact factor: 3.422

6.  Enteropathogenic Escherichia coli strains carrying genes encoding the PER-2 and TEM-116 extended-spectrum beta-lactamases isolated from children with diarrhea in Uruguay.

Authors:  Rafael Vignoli; Gustavo Varela; María Inés Mota; Nicolas F Cordeiro; Pablo Power; Elizabet Ingold; Pilar Gadea; Alfredo Sirok; Felipe Schelotto; Juan A Ayala; Gabriel Gutkind
Journal:  J Clin Microbiol       Date:  2005-06       Impact factor: 5.948

7.  Predominance of afr2 and ral fimbrial genes related to those encoding the K88 and CS31A fimbrial adhesins in enteropathogenic Escherichia coli isolates from rabbits with postweaning diarrhea in Central Europe.

Authors:  Mohamed A Dow; István Tóth; Pavel Alexa; Michael Davies; Anna Malik; Eric Oswald; Béla Nagy
Journal:  J Clin Microbiol       Date:  2005-03       Impact factor: 5.948

8.  Monoclonal antibodies specific for the bundle-forming pilus of enteropathogenic Escherichia coli.

Authors:  J A Girón; F Qadri; T Azim; K J Jarvis; J B Kaper; M J Albert
Journal:  Infect Immun       Date:  1995-12       Impact factor: 3.441

9.  Rapid identification of Escherichia coli pathotypes by virulence gene detection with DNA microarrays.

Authors:  Sadjia Bekal; Roland Brousseau; Luke Masson; Gabrielle Prefontaine; John Fairbrother; Josée Harel
Journal:  J Clin Microbiol       Date:  2003-05       Impact factor: 5.948

10.  Characterization of atypical enteropathogenic Escherichia coli strains harboring the astA gene that were associated with a waterborne outbreak of diarrhea in Japan.

Authors:  Jun Yatsuyanagi; Shioko Saito; Yoshimichi Miyajima; Ken-Ichi Amano; Katsuhiko Enomoto
Journal:  J Clin Microbiol       Date:  2003-05       Impact factor: 5.948
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