Literature DB >> 7567460

Genotypic analysis of multiple loci in somatic cells by whole genome amplification.

M T Barrett1, B J Reid, G Joslyn.   

Abstract

To screen multiple loci in small purified samples of diploid and aneuploid cells a PCR-based technique of whole genome amplification was adapted to the study of somatic lesions. DNA samples from different numbers of flow-sorted diploid and aneuploid cells from biopsies were amplified with a degenerate 15mer primer. Aliquots of these reactions were then used in locus-specific reactions using a single round of PCR cycles with individual sets of primers representing polymorphic markers for different regions. As a result, polymorphic markers for different chromosomal regions, including VNTRs and dinucleotide repeats, can be used to perform up to 30 locus-specific PCR assays with a single sample obtained from fewer than 1000 cells.

Entities:  

Mesh：

Substances：

Year:  1995        PMID： 7567460      PMCID： PMC307228          DOI： 10.1093/nar/23.17.3488

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

1.  Dinucleotide repeat polymorphisms at the D8S85, D8S87, and D8S88 loci.

Authors:  J L Weber; A E Kwitek; P E May; D Patterson; H Drabkin
Journal:  Nucleic Acids Res       Date:  1990-07-11       Impact factor: 16.971

2.  Preimplantation single-cell analysis of multiple genetic loci by whole-genome amplification.

Authors:  M C Snabes; S S Chong; S B Subramanian; K Kristjansson; D DiSepio; M R Hughes
Journal:  Proc Natl Acad Sci U S A       Date:  1994-06-21       Impact factor: 11.205

3.  17p allelic losses in diploid cells of patients with Barrett's esophagus who develop aneuploidy.

Authors:  P L Blount; P C Galipeau; C A Sanchez; K Neshat; D S Levine; J Yin; H Suzuki; J M Abraham; S J Meltzer; B J Reid
Journal:  Cancer Res       Date:  1994-05-01       Impact factor: 12.701

4.  Mutation in the DNA mismatch repair gene homologue hMLH1 is associated with hereditary non-polyposis colon cancer.

Authors:  C E Bronner; S M Baker; P T Morrison; G Warren; L G Smith; M K Lescoe; M Kane; C Earabino; J Lipford; A Lindblom
Journal:  Nature       Date:  1994-03-17       Impact factor: 49.962

5.  Genetic alterations during colorectal-tumor development.

Authors:  B Vogelstein; E R Fearon; S R Hamilton; S E Kern; A C Preisinger; M Leppert; Y Nakamura; R White; A M Smits; J L Bos
Journal:  N Engl J Med       Date:  1988-09-01       Impact factor: 91.245

6.  Frequent inactivation of the retinoblastoma anti-oncogene is restricted to a subset of human tumor cells.

Authors:  J M Horowitz; S H Park; E Bogenmann; J C Cheng; D W Yandell; F J Kaye; J D Minna; T P Dryja; R A Weinberg
Journal:  Proc Natl Acad Sci U S A       Date:  1990-04       Impact factor: 11.205

Review 7.  p53 mutations in human cancers.

Authors:  M Hollstein; D Sidransky; B Vogelstein; C C Harris
Journal:  Science       Date:  1991-07-05       Impact factor: 47.728

8.  Identification of a chromosome 18q gene that is altered in colorectal cancers.

Authors:  E R Fearon; K R Cho; J M Nigro; S E Kern; J W Simons; J M Ruppert; S R Hamilton; A C Preisinger; G Thomas; K W Kinzler
Journal:  Science       Date:  1990-01-05       Impact factor: 47.728

9.  Clonal ordering of 17p and 5q allelic losses in Barrett dysplasia and adenocarcinoma.

Authors:  P L Blount; S J Meltzer; J Yin; Y Huang; M J Krasna; B J Reid
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-15       Impact factor: 11.205

10.  The human mutator gene homolog MSH2 and its association with hereditary nonpolyposis colon cancer.

Authors:  R Fishel; M K Lescoe; M R Rao; N G Copeland; N A Jenkins; J Garber; M Kane; R Kolodner
Journal:  Cell       Date:  1993-12-03       Impact factor: 41.582
View more
  11 in total

1.  Single nucleotide polymorphism array analysis of flow-sorted epithelial cells from frozen versus fixed tissues for whole genome analysis of allelic loss in breast cancer.

Authors:  Elizabeth L Schubert; Li Hsu; Laura A Cousens; Jeri Glogovac; Steve Self; Brian J Reid; Peter S Rabinovitch; Peggy L Porter
Journal:  Am J Pathol       Date:  2002-01       Impact factor: 4.307

2.  Loss of heterozygosity analysis using whole genome amplification, cell sorting, and fluorescence-based PCR.

Authors:  T G Paulson; P C Galipeau; B J Reid
Journal:  Genome Res       Date:  1999-05       Impact factor: 9.043

3.  17p (p53) allelic losses, 4N (G2/tetraploid) populations, and progression to aneuploidy in Barrett's esophagus.

Authors:  P C Galipeau; D S Cowan; C A Sanchez; M T Barrett; M J Emond; D S Levine; P S Rabinovitch; B J Reid
Journal:  Proc Natl Acad Sci U S A       Date:  1996-07-09       Impact factor: 11.205

4.  Whole genome amplification using a degenerate oligonucleotide primer allows hundreds of genotypes to be performed on less than one nanogram of genomic DNA.

Authors:  V G Cheung; S F Nelson
Journal:  Proc Natl Acad Sci U S A       Date:  1996-12-10       Impact factor: 11.205

5.  Predictors of progression in Barrett's esophagus II: baseline 17p (p53) loss of heterozygosity identifies a patient subset at increased risk for neoplastic progression.

Authors:  B J Reid; L J Prevo; P C Galipeau; C A Sanchez; G Longton; D S Levine; P L Blount; P S Rabinovitch
Journal:  Am J Gastroenterol       Date:  2001-10       Impact factor: 10.864

6.  Whole genome amplification of single cells from clinical peripheral blood smears.

Authors:  C P Beltinger; F Klimek; K M Debatin
Journal:  Mol Pathol       Date:  1997-10

7.  Molecular analysis of microdissected tumors and preneoplastic intraductal lesions in pancreatic carcinoma.

Authors:  E Heinmöller; W Dietmaier; H Zirngibl; P Heinmöller; W Scaringe; K W Jauch; F Hofstädter; J Rüschoff
Journal:  Am J Pathol       Date:  2000-07       Impact factor: 4.307

8.  Cell proliferation, cell cycle abnormalities, and cancer outcome in patients with Barrett's esophagus: a long-term prospective study.

Authors:  Dennis L Chao; Carissa A Sanchez; Patricia C Galipeau; Patricia L Blount; Thomas G Paulson; David S Cowan; Kamran Ayub; Robert D Odze; Peter S Rabinovitch; Brian J Reid
Journal:  Clin Cancer Res       Date:  2008-11-01       Impact factor: 12.531

9.  Translation of an STR-based biomarker into a clinically compatible SNP-based platform for loss of heterozygosity.

Authors:  Heather D Kissel; Patricia C Galipeau; Xiaohong Li; Brian J Reid
Journal:  Cancer Biomark       Date:  2009       Impact factor: 4.388

10.  Multiple mutation analyses in single tumor cells with improved whole genome amplification.

Authors:  W Dietmaier; A Hartmann; S Wallinger; E Heinmöller; T Kerner; E Endl; K W Jauch; F Hofstädter; J Rüschoff
Journal:  Am J Pathol       Date:  1999-01       Impact factor: 4.307
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.