Literature DB >> 7551552

Imaging of DNA in human and plant chromosomes by high-resolution scanning electron microscopy.

G Wanner1, H Formanek.   

Abstract

We describe a DNA-specific staining procedure, using a blue platinum organic dye, which allows DNA imaging of chromosomes by detection of back-scattered electrons in the scanning electron microscope. DNA distribution is visualized within chromosomal details of the centromeric and satellite regions, or in interphase chromatin, with high-resolution (10-15 nm) for comparison with the corresponding secondary electron image representing DNA plus protein.

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Year:  1995        PMID： 7551552     DOI： 10.1007/bf00710018

Source DB:  PubMed          Journal:  Chromosome Res        ISSN： 0967-3849            Impact factor:   5.239


  11 in total

1.  The organization of the mammalian kinetochore: a scanning electron microscope study.

Authors:  J B Rattner
Journal:  Chromosoma       Date:  1987       Impact factor: 4.316

2.  High resolution detection of uncoated metaphase chromosomes by means of field emission scanning electron microscopy.

Authors:  R Rizzoli; E Rizzi; M Falconi; A Galanzi; B Baratta; G Lattanzi; M Vitale; G Mazzotti; L Manzoli
Journal:  Chromosoma       Date:  1994-10       Impact factor: 4.316

3.  Scanning electron microscopy of polytene chromosomes (I).

Authors:  C Pelling; T D Allen
Journal:  Chromosome Res       Date:  1993-11       Impact factor: 5.239

4.  Efficient preparation of plant chromosomes for high-resolution scanning electron microscopy.

Authors:  R Martin; W Busch; R G Herrmann; G Wanner
Journal:  Chromosome Res       Date:  1994-09       Impact factor: 5.239

5.  Scanning electron microscopy of the centromeric region of L-cell chromosomes after treatment with Hoechst 33258 combined with 5-bromodeoxyuridine.

Authors:  S Takayama; H Hiramatsu
Journal:  Chromosoma       Date:  1993-03       Impact factor: 4.316

6.  [Cytostatic platinum complexes: an unexpected discovery with considerable consequences].

Authors:  P Köpf-Maier; H Köpf
Journal:  Naturwissenschaften       Date:  1986-05

7.  Scanning electron microscopy of mammalian chromosomes from prophase to telophase.

Authors:  A T Sumner
Journal:  Chromosoma       Date:  1991-07       Impact factor: 4.316

8.  Cell-surface-associated nucleic acid in tumorigenic cells made visible with platinum-pyrimidine complexes by electron microscopy.

Authors:  S K Aggarwal; R W Wagner; P K McAllister; B Rosenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205

9.  Scanning electron microscope analysis of structural changes and aberrations in human chromosomes associated with the inhibition and reversal of inhibition of ultraviolet light induced DNA repair.

Authors:  A M Mullinger; R T Johnson
Journal:  Chromosoma       Date:  1987       Impact factor: 4.316

10.  High-resolution scanning electron microscopy of human metaphase chromosomes.

Authors:  C J Harrison; T D Allen; M Britch; R Harris
Journal:  J Cell Sci       Date:  1982-08       Impact factor: 5.285
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  12 in total

1.  Characterization of a peg-like terminal NOR structure with light microscopy and high-resolution scanning electron microscopy.

Authors:  Elizabeth Schroeder-Reiter; Andreas Houben; Jürke Grau; Gerhard Wanner
Journal:  Chromosoma       Date:  2005-11-03       Impact factor: 4.316

2.  Chromosome imaging by atomic force microscopy: influencing factors and comparative evaluation.

Authors:  Yangzhe Wu; Jiye Cai; Longqiu Cheng; Chenxi Wang; Yong Chen
Journal:  J Genet       Date:  2006-08       Impact factor: 1.166

3.  Genomic instability within centromeres of interspecific marsupial hybrids.

Authors:  Cushla J Metcalfe; Kira V Bulazel; Gianni C Ferreri; Elizabeth Schroeder-Reiter; Gerhard Wanner; Willem Rens; Craig Obergfell; Mark D B Eldridge; Rachel J O'Neill
Journal:  Genetics       Date:  2007-12       Impact factor: 4.562

4.  Chromosome inner structure investigation by electron tomography and electron diffraction in a transmission electron microscope.

Authors:  Rinyaporn Phengchat; Marek Malac; Misa Hayashida
Journal:  Chromosome Res       Date:  2021-03-18       Impact factor: 5.239

5.  Globular and fibrous structure in barley chromosomes revealed by high-resolution scanning electron microscopy.

Authors:  M Iwano; K Fukui; S Takaichi; A Isogai
Journal:  Chromosome Res       Date:  1997-08       Impact factor: 5.239

6.  RICE MORPHOLOGY DETERMINANT encodes the type II formin FH5 and regulates rice morphogenesis.

Authors:  Zheng Zhang; Yi Zhang; Hexin Tan; Ying Wang; Gang Li; Wanqi Liang; Zheng Yuan; Jianping Hu; Haiyun Ren; Dabing Zhang
Journal:  Plant Cell       Date:  2011-02-09       Impact factor: 11.277

7.  Structural organization of very small chromosomes: study on a single-celled evolutionary distant eukaryote Giardia intestinalis.

Authors:  Pavla Tůmová; Magdalena Uzlíková; Gerhard Wanner; Eva Nohýnková
Journal:  Chromosoma       Date:  2014-08-30       Impact factor: 4.316

8.  Nuclear incorporation of iron during the eukaryotic cell cycle.

Authors:  Ian Robinson; Yang Yang; Fucai Zhang; Christophe Lynch; Mohammed Yusuf; Peter Cloetens
Journal:  J Synchrotron Radiat       Date:  2016-10-18       Impact factor: 2.616

9.  Immunogold labeling of chromosomes for scanning electron microscopy: a closer look at phosphorylated histone H3 in mitotic metaphase chromosomes of Hordeum vulgare.

Authors:  E Schroeder-Reiter; A Houben; G Wanner
Journal:  Chromosome Res       Date:  2003       Impact factor: 4.620

10.  Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution.

Authors:  Hanfei Yan; Evgeny Nazaretski; Kenneth Lauer; Xiaojing Huang; Ulrich Wagner; Christoph Rau; Mohammed Yusuf; Ian Robinson; Sebastian Kalbfleisch; Li Li; Nathalie Bouet; Juan Zhou; Ray Conley; Yong S Chu
Journal:  Sci Rep       Date:  2016-02-05       Impact factor: 4.379
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