Literature DB >> 7981946

Efficient preparation of plant chromosomes for high-resolution scanning electron microscopy.

R Martin1, W Busch, R G Herrmann, G Wanner.   

Abstract

A highly reproducible technique to prepare plant chromosomes for high-resolution field emission scanning electron microscopy is presented. The procedure allows the production of relatively high numbers of chromosome spreads that can be viewed at high resolution, showing structural details below 10 nm. This preparation technique is not restricted to metaphase chromosomes, but also allows the observation of plant chromosomes during all stages of the cell cycle.

Mesh：

Year:  1994        PMID： 7981946     DOI： 10.1007/BF01552801

Source DB:  PubMed          Journal:  Chromosome Res        ISSN： 0967-3849            Impact factor:   5.239


  7 in total

1.  Preparation of human chromosomes for high resolution scanning electron microscopy.

Authors:  P O Kättström; B O Nilsson
Journal:  Arch Histol Cytol       Date:  1992

2.  Highly effective cell synchronization in plant roots by hydroxyurea and amiprophos-methyl or colchicine.

Authors:  W H Pan; A Houben; R Schlegel
Journal:  Genome       Date:  1993-04       Impact factor: 2.166

3.  The organization of the mammalian kinetochore: a scanning electron microscope study.

Authors:  J B Rattner
Journal:  Chromosoma       Date:  1987       Impact factor: 4.316

4.  Sensitivity enhancement of fluorescence in situ hybridization on plant chromosomes.

Authors:  W Busch; R Martin; R G Herrmann
Journal:  Chromosome Res       Date:  1994-01       Impact factor: 5.239

5.  Microdissection and microcloning of the barley (Hordeum vulgare L.) chromosome 1HS.

Authors:  J Schondelmaier; R Martin; A Jahoor; A Houben; A Graner; H U Koop; R G Herrmann; C Jung
Journal:  Theor Appl Genet       Date:  1993-06       Impact factor: 5.699

6.  Scanning electron microscopy of mammalian chromosomes from prophase to telophase.

Authors:  A T Sumner
Journal:  Chromosoma       Date:  1991-07       Impact factor: 4.316

7.  High-resolution scanning electron microscopy of human metaphase chromosomes.

Authors:  C J Harrison; T D Allen; M Britch; R Harris
Journal:  J Cell Sci       Date:  1982-08       Impact factor: 5.285
  7 in total
  14 in total

1.  Analysis by atomic force microscopy of morphological changes in barley chromosomes during FISH treatment.

Authors:  Motoharu Shichiri; Daisuke Fukushi; Shigeru Sugiyama; Tomoyuki Yoshino; Toshio Ohtani
Journal:  Chromosome Res       Date:  2003       Impact factor: 5.239

2.  A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants.

Authors:  Lala Aliyeva-Schnorr; Lu Ma; Andreas Houben
Journal:  J Vis Exp       Date:  2015-12-16       Impact factor: 1.355

3.  Characterization of a peg-like terminal NOR structure with light microscopy and high-resolution scanning electron microscopy.

Authors:  Elizabeth Schroeder-Reiter; Andreas Houben; Jürke Grau; Gerhard Wanner
Journal:  Chromosoma       Date:  2005-11-03       Impact factor: 4.316

4.  Genomic instability within centromeres of interspecific marsupial hybrids.

Authors:  Cushla J Metcalfe; Kira V Bulazel; Gianni C Ferreri; Elizabeth Schroeder-Reiter; Gerhard Wanner; Willem Rens; Craig Obergfell; Mark D B Eldridge; Rachel J O'Neill
Journal:  Genetics       Date:  2007-12       Impact factor: 4.562

5.  The ultrastructure of mono- and holocentric plant centromeres: an immunological investigation by structured illumination microscopy and scanning electron microscopy.

Authors:  Gerhard Wanner; Elizabeth Schroeder-Reiter; Wei Ma; Andreas Houben; Veit Schubert
Journal:  Chromosoma       Date:  2015-06-06       Impact factor: 4.316

6.  Changes in chromosomal ultrastructure during the cell cycle.

Authors:  R Martin; W Busch; R G Herrmann; G Wanner
Journal:  Chromosome Res       Date:  1996-06       Impact factor: 5.239

7.  Fluorescence in situ hybridization on plant extended chromatin DNA fibers for single-copy and repetitive DNA sequences.

Authors:  Kun Yang; Hecui Zhang; Richard Converse; Yong Wang; Xiaoying Rong; Zhigang Wu; Bing Luo; Liyan Xue; Li Jian; Liquan Zhu; Xiaojia Wang
Journal:  Plant Cell Rep       Date:  2011-06-22       Impact factor: 4.570

8.  Globular and fibrous structure in barley chromosomes revealed by high-resolution scanning electron microscopy.

Authors:  M Iwano; K Fukui; S Takaichi; A Isogai
Journal:  Chromosome Res       Date:  1997-08       Impact factor: 5.239

9.  Imaging of DNA in human and plant chromosomes by high-resolution scanning electron microscopy.

Authors:  G Wanner; H Formanek
Journal:  Chromosome Res       Date:  1995-09       Impact factor: 5.239

10.  Structural organization of very small chromosomes: study on a single-celled evolutionary distant eukaryote Giardia intestinalis.

Authors:  Pavla Tůmová; Magdalena Uzlíková; Gerhard Wanner; Eva Nohýnková
Journal:  Chromosoma       Date:  2014-08-30       Impact factor: 4.316
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