Literature DB >> 7542273

Identification of Campylobacter fetus by PCR-DNA probe method.

K Blom1, C M Patton, M A Nicholson, B Swaminathan.   

Abstract

A PCR method for rapid identification of Campylobacter fetus subsp. fetus was evaluated. A fragment of the gene coding for 16S rRNA was amplified from crude cell lysates of 18 C. fetus strains and 30 strains representing other Campylobacter species and subspecies. The amplicons were probed by dot blot hybridization with a digoxigenin-labeled C. fetus-specific oligonucleotide probe. The probe reacted only with C. fetus subsp. fetus and C. fetus subsp. venerealis and may be useful for rapid identification in clinical laboratories.

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Year:  1995        PMID： 7542273      PMCID： PMC228165          DOI： 10.1128/jcm.33.5.1360-1362.1995

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction.

Authors:  K H Wilson; R B Blitchington; R C Greene
Journal:  J Clin Microbiol       Date:  1990-09       Impact factor: 5.948

2.  Oligodeoxynucleotide probes for Campylobacter fetus and Campylobacter hyointestinalis based on 16S rRNA sequences.

Authors:  I V Wesley; R D Wesley; M Cardella; F E Dewhirst; B J Paster
Journal:  J Clin Microbiol       Date:  1991-09       Impact factor: 5.948

3.  Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.

Authors:  W I Wood; J Gitschier; L A Lasky; R M Lawn
Journal:  Proc Natl Acad Sci U S A       Date:  1985-03       Impact factor: 11.205

4.  Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.

Authors:  F C Kafatos; C W Jones; A Efstratiadis
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

5.  Flagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis.

Authors:  I Nachamkin; K Bohachick; C M Patton
Journal:  J Clin Microbiol       Date:  1993-06       Impact factor: 5.948

6.  "Campylobacter hyointestinalis" sp. nov.: a new species of Campylobacter found in the intestines of pigs and other animals.

Authors:  C J Gebhart; P Edmonds; G E Ward; H J Kurtz; D J Brenner
Journal:  J Clin Microbiol       Date:  1985-05       Impact factor: 5.948

7.  Campylobacter fetus diarrhea in a Hutterite colony: epidemiological observations and typing of the causative organism.

Authors:  R P Rennie; D Strong; D E Taylor; S M Salama; C Davidson; H Tabor
Journal:  J Clin Microbiol       Date:  1994-03       Impact factor: 5.948

8.  Campylobacter infection associated with raw milk. An outbreak of gastroenteritis due to Campylobacter jejuni and thermotolerant Campylobacter fetus subsp fetus.

Authors:  B S Klein; J M Vergeront; M J Blaser; P Edmonds; D J Brenner; D Janssen; J P Davis
Journal:  JAMA       Date:  1986-01-17       Impact factor: 56.272
  8 in total
  5 in total

1.  Rapid identification of Campylobacter species by restriction fragment length polymorphism analysis of a PCR-amplified fragment of the gene coding for 16S rRNA.

Authors:  P Cardarelli-Leite; K Blom; C M Patton; M A Nicholson; A G Steigerwalt; S B Hunter; D J Brenner; T J Barrett; B Swaminathan
Journal:  J Clin Microbiol       Date:  1996-01       Impact factor: 5.948

Review 2.  Identification methods for campylobacters, helicobacters, and related organisms.

Authors:  S L On
Journal:  Clin Microbiol Rev       Date:  1996-07       Impact factor: 26.132

3.  Colony multiplex PCR assay for identification and differentiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. fetus.

Authors:  Gehua Wang; Clifford G Clark; Tracy M Taylor; Chad Pucknell; Connie Barton; Lawrence Price; David L Woodward; Frank G Rodgers
Journal:  J Clin Microbiol       Date:  2002-12       Impact factor: 5.948

4.  A novel real-time PCR assay for quantitative detection of Campylobacter fetus based on ribosomal sequences.

Authors:  Gregorio Iraola; Ruben Pérez; Laura Betancor; Ana Marandino; Claudia Morsella; Alejandra Méndez; Fernando Paolicchi; Alessandra Piccirillo; Gonzalo Tomás; Alejandra Velilla; Lucía Calleros
Journal:  BMC Vet Res       Date:  2016-12-15       Impact factor: 2.741

5.  Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence.

Authors:  Rafael Delpiazzo; Maila Barcellos; Sofía Barros; Laura Betancor; Martín Fraga; Jorge Gil; Gregorio Iraola; Claudia Morsella; Fernando Paolicchi; Ruben Pérez; Franklin Riet-Correa; Margarita Sanguinetti; Alfonso Silva; Caroline da Silva Silveira; Lucía Calleros
Journal:  Vet Anim Sci       Date:  2020-12-24
  5 in total

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