Literature DB >> 12454184

Colony multiplex PCR assay for identification and differentiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. fetus.

Gehua Wang1, Clifford G Clark, Tracy M Taylor, Chad Pucknell, Connie Barton, Lawrence Price, David L Woodward, Frank G Rodgers.   

Abstract

A multiplex PCR assay was used to simultaneously detect genes from the five major clinically relevant Campylobacter spp. Those genes selected were hipO and 23S rRNA from Campylobacter jejuni; glyA from each of C. coli, C. lari, and C. upsaliensis; and sapB2 from C. fetus subsp. fetus. The assay was evaluated with 137 clinical and environmental isolates and was found to be rapid and easy to perform and had a high sensitivity and specificity for characterizing isolates, even in mixed cultures.

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Year:  2002        PMID： 12454184      PMCID： PMC154608          DOI： 10.1128/JCM.40.12.4744-4747.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  21 in total

1.  PCR detection, identification to species level, and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples.

Authors:  D Linton; A J Lawson; R J Owen; J Stanley
Journal:  J Clin Microbiol       Date:  1997-10       Impact factor: 5.948

2.  Restriction fragment length polymorphism analysis shows that the hippuricase gene of Campylobacter jejuni is highly conserved.

Authors:  E R Slater; R J Owen
Journal:  Lett Appl Microbiol       Date:  1997-10       Impact factor: 2.858

3.  Detection of thermophilic Campylobacter from sparrows by multiplex PCR: the role of sparrows as a source of contamination of broilers with Campylobacter.

Authors:  T Chuma; S Hashimoto; K Okamoto
Journal:  J Vet Med Sci       Date:  2000-12       Impact factor: 1.267

4.  Campylobacter contamination of raw meat and poultry at retail sale: identification of multiple types and comparison with isolates from human infection.

Authors:  J M Kramer; J A Frost; F J Bolton; D R Wareing
Journal:  J Food Prot       Date:  2000-12       Impact factor: 2.077

5.  Rapid detection of Campylobacter fetus by polymerase chain reaction combined with non-radioactive hybridization using an oligonucleotide covalently bound to microwells.

Authors:  I Casadémont; C Bizet; D Chevrier; J L Guesdon
Journal:  Mol Cell Probes       Date:  2000-08       Impact factor: 2.365

6.  Development of a m-PCR assay for simultaneous identification of Campylobacter jejuni and C. coli.

Authors:  M Denis; C Soumet; K Rivoal; G Ermel; D Blivet; G Salvat; P Colin
Journal:  Lett Appl Microbiol       Date:  1999-12       Impact factor: 2.858

7.  Specific identification of Campylobacter fetus by PCR targeting variable regions of the 16S rDNA.

Authors:  O A Oyarzabal; I V Wesley; K M Harmon; L Schroeder-Tucker; J M Barbaree; L H Lauerman; S Backert; D E Conner
Journal:  Vet Microbiol       Date:  1997-10-31       Impact factor: 3.293

8.  Rapid identification of thermotolerant Campylobacter jejuni, Campylobacter coli, Campylobacter lari, and Campylobacter upsaliensis from various geographic locations by a GTPase-based PCR-reverse hybridization assay.

Authors:  L J van Doorn; A Verschuuren-van Haperen; A Burnens; M Huysmans; P Vandamme; B A Giesendorf; M J Blaser; W G Quint
Journal:  J Clin Microbiol       Date:  1999-06       Impact factor: 5.948

9.  Rapid PCR with nested primers for direct detection of Campylobacter jejuni in chicken washes.

Authors:  D K Winters; A E O'Leary; M F Slavik
Journal:  Mol Cell Probes       Date:  1997-08       Impact factor: 2.365

10.  Cloning of a sapB homologue (sapB2) encoding a putative 112-kDa Campylobacter fetus S-layer protein and its use for identification and molecular genotyping.

Authors:  I Casadémont; D Chevrier; J L Guesdon
Journal:  FEMS Immunol Med Microbiol       Date:  1998-08
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  93 in total

1.  Comprehensive detection and discrimination of Campylobacter species by use of confocal micro-Raman spectroscopy and multilocus sequence typing.

Authors:  Xiaonan Lu; Qian Huang; William G Miller; D Eric Aston; Jie Xu; Feng Xue; Hongwei Zhang; Barbara A Rasco; Shuo Wang; Michael E Konkel
Journal:  J Clin Microbiol       Date:  2012-06-27       Impact factor: 5.948

2.  Survival of Campylobacter spp. in darkling beetles (Alphitobius diaperinus) and their larvae in Australia.

Authors:  Jillian M Templeton; Amanda J De Jong; P J Blackall; Jeanette K Miflin
Journal:  Appl Environ Microbiol       Date:  2006-09-29       Impact factor: 4.792

3.  Antibiotic resistance of retail food and human Campylobacter isolates on the island of Ireland from 2001-2002.

Authors:  K McGill; D Cowley; L Moran; P Scates; A O'Leary; R H Madden; C Carroll; E McNamara; J E Moore; S Fanning; J D Collins; P Whyte
Journal:  Epidemiol Infect       Date:  2006-04-20       Impact factor: 2.451

4.  Salmonella, Campylobacter and Enterococcus spp.: their antimicrobial resistance profiles and their spatial relationships in a synoptic study of the Upper Oconee River basin.

Authors:  R J Meinersmann; M E Berrang; C R Jackson; P Fedorka-Cray; S Ladely; E Little; J G Frye; B Mattsson
Journal:  Microb Ecol       Date:  2007-08-09       Impact factor: 4.552

5.  Speciation of Campylobacter coli, C. jejuni, C. helveticus, C. lari, C. sputorum, and C. upsaliensis by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Authors:  Robert E Mandrell; Leslie A Harden; Anna Bates; William G Miller; William F Haddon; Clifton K Fagerquist
Journal:  Appl Environ Microbiol       Date:  2005-10       Impact factor: 4.792

6.  Antimicrobial susceptibility profiles and molecular typing of Campylobacter jejuni and Campylobacter coli isolates from ducks in South Korea.

Authors:  Bai Wei; Se-Yeoun Cha; Min Kang; Jae-Hee Roh; Hye-Suk Seo; Ran-Hee Yoon; Hyung-Kwan Jang
Journal:  Appl Environ Microbiol       Date:  2014-09-26       Impact factor: 4.792

7.  Isolation of Campylobacter spp. from Three Species of Antarctic Penguins in Different Geographic Locations.

Authors:  F J García-Peña; M T Llorente; T Serrano; M J Ruano; J Belliure; J Benzal; S Herrera-León; V Vidal; V D'Amico; D Pérez-Boto; A Barbosa
Journal:  Ecohealth       Date:  2017-01-13       Impact factor: 3.184

8.  The in vitro susceptibility of Campylobacter spp. to the antibacterial effect of manuka honey.

Authors:  S M Lin; P C Molan; R T Cursons
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2008-09-26       Impact factor: 3.267

9.  Identification of thermotolerant campylobacter species by fluorescence in situ hybridization.

Authors:  Sven Poppert; Michaela Haas; Tatjana Yildiz; Thomas Alter; Edda Bartel; Ursula Fricke; Andreas Essig
Journal:  J Clin Microbiol       Date:  2008-04-02       Impact factor: 5.948

10.  Molecular epidemiology and characterization of Campylobacter spp. isolated from wild bird populations in northern England.

Authors:  Laura A Hughes; Malcolm Bennett; Peter Coffey; John Elliott; Trevor R Jones; Richard C Jones; Angela Lahuerta-Marin; A Howard Leatherbarrow; Kenny McNiffe; David Norman; Nicola J Williams; Julian Chantrey
Journal:  Appl Environ Microbiol       Date:  2009-03-13       Impact factor: 4.792
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