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Literature DB >> 7542270

Diagnostic assay for Helicobacter hepaticus based on nucleotide sequence of its 16S rRNA gene.

J K Battles¹, J C Williamson, K M Pike, P L Gorelick, J M Ward, M A Gonda.

Abstract

Conserved primers were used to PCR amplify 95% of the Helicobacter hepaticus 16S rRNA gene. Its sequence was determined and aligned to those of related bacteria, enabling the selection of primers to highly diverged regions of the 16S rRNA gene and an oligonucleotide probe for the development of a PCR-liquid hybridization assay. This assay was shown to be both sensitive and specific for H. hepaticus 16S rRNA gene sequences.

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Year: 1995 PMID： 7542270 PMCID： PMC228160 DOI： 10.1128/jcm.33.5.1344-1347.1995

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

39 in total

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Journal: J Clin Microbiol Date: 1991-11 Impact factor: 5.948

2. Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA.

Authors: U Edwards; T Rogall; H Blöcker; M Emde; E C Böttger
Journal: Nucleic Acids Res Date: 1989-10-11 Impact factor: 16.971

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5. Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov.

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9. Helicobacter nemestrinae sp. nov., a spiral bacterium found in the stomach of a pigtailed macaque (Macaca nemestrina)

Authors: M A Bronsdon; C S Goodwin; L I Sly; T Chilvers; F D Schoenknecht
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10. Oligonucleotide probe for detection and identification of Campylobacter pylori.

Authors: M Morotomi; S Hoshina; P Green; H C Neu; P LoGerfo; I Watanabe; M Mutai; I B Weinstein
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3. Lurking in the shadows: emerging rodent infectious diseases.

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Authors: S L On
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5. Helicobacter hepaticus, a recently recognized bacterial pathogen, associated with chronic hepatitis and hepatocellular neoplasia in laboratory mice.

Authors: J M Rice
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6. Helicobacter hepaticus-induced colitis in interleukin-10-deficient mice: cytokine requirements for the induction and maintenance of intestinal inflammation.

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7. Fecal PCR assay for diagnosis of Helicobacter infection in laboratory rodents.

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Journal: J Clin Microbiol Date: 1997-06 Impact factor: 5.948

8. Identification of murine helicobacters by PCR and restriction enzyme analyses.

Authors: L K Riley; C L Franklin; R R Hook; C Besch-Williford
Journal: J Clin Microbiol Date: 1996-04 Impact factor: 5.948

9. Helicobacter hepaticus triggers colitis in specific-pathogen-free interleukin-10 (IL-10)-deficient mice through an IL-12- and gamma interferon-dependent mechanism.

Authors: M C Kullberg; J M Ward; P L Gorelick; P Caspar; S Hieny; A Cheever; D Jankovic; A Sher
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10. CD4+CD45RBHi interleukin-4 defective T cells elicit antral gastritis and duodenitis.

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