Literature DB >> 9163500

Fecal PCR assay for diagnosis of Helicobacter infection in laboratory rodents.

C S Beckwith1, C L Franklin, R R Hook, C L Besch-Williford, L K Riley.   

Abstract

A fecal PCR assay for detection of Helicobacter infections in laboratory rodents was developed. DNA was isolated from murine fecal pellets, and a region of the 16S rRNA gene conserved among murine Helicobacter species was amplified. The fecal PCR was sensitive and specific. This assay does not require euthanasia of rodents, which is especially important for valuable rodents, such as transgenic mice.

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Year:  1997        PMID： 9163500      PMCID： PMC229805          DOI： 10.1128/jcm.35.6.1620-1623.1997

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


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