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Literature DB >> 7542269

Detection of the turkey pathogens Mycoplasma meleagridis and M. iowae by amplification of genes coding for rRNA.

Abstract

PCR-based diagnostic tests using oligonucleotides specific to 16S rRNA were designed for the specific detection of the turkey pathogens Mycoplasma meleagridis and M. iowae. This method of detection was shown to be rapid, species specific, and unaffected by strain variation or the presence of other organisms. Detection of M. meleagridis in clinical samples by PCR was achieved and later confirmed by culture and growth inhibition. Definitive identification by culture and growth inhibition required up to 3 weeks, whereas positive results from PCR testing were obtained within a day and negative samples were confirmed within 4 days.

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Year: 1995 PMID： 7542269 PMCID： PMC228157 DOI： 10.1128/jcm.33.5.1335-1338.1995

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

16 in total

1. Identification of a plant-derived mollicute as a strain of an avian pathogen, Mycoplasma iowae, and its implications for mollicute taxonomy.

Authors: O Grau; F Laigret; P Carle; J G Tully; D L Rose; J M Bové
Journal: Int J Syst Bacteriol Date: 1991-10

1. Duplex PCR to differentiate between Mycoplasma synoviae and Mycoplasma gallisepticum on the basis of conserved species-specific sequences of their hemagglutinin genes.

Authors: B Ben Abdelmoumen Mardassi; R Ben Mohamed; I Gueriri; S Boughattas; B Mlik
Journal: J Clin Microbiol Date: 2005-02 Impact factor: 5.948

1 in total

Detection of the turkey pathogens Mycoplasma meleagridis and M. iowae by amplification of genes coding for rRNA.

1. Identification of a plant-derived mollicute as a strain of an avian pathogen, Mycoplasma iowae, and its implications for mollicute taxonomy.

2. Isolation of Mycoplasma gallinarum and M. gallinaceum from the reproductive tract of hens.

3. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

4. Mycoplasma meleagridis of turkeys: probability of eliminating egg-borne infection.

5. Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification.

6. Detection of Mycoplasma meleagridis by polymerase chain reaction.

7. Antigenic heterogeneity in Mycoplasma iowae demonstrated with monoclonal antibodies.

8. Detection and identification of mycoplasmas by amplification of rDNA.

9. Comparison of seven isolates of Mycoplasma meleagridis.

10. Antigenic analysis of three strains of Mycoplasma meleagridis of varying pathogenicity.

1. Duplex PCR to differentiate between Mycoplasma synoviae and Mycoplasma gallisepticum on the basis of conserved species-specific sequences of their hemagglutinin genes.