Literature DB >> 15695715

Duplex PCR to differentiate between Mycoplasma synoviae and Mycoplasma gallisepticum on the basis of conserved species-specific sequences of their hemagglutinin genes.

B Ben Abdelmoumen Mardassi1, R Ben Mohamed, I Gueriri, S Boughattas, B Mlik.   

Abstract

We developed a duplex PCR assay targeting the hemagglutinin multigene families, vlhA and pMGA, of Mycoplasma synoviae and Mycoplasma gallisepticum, respectively. The assay proved to be specific and sensitive enough to justify its use for the simultaneous detection of the two major avian mycoplasma species from field isolates.

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Year:  2005        PMID： 15695715      PMCID： PMC548031          DOI： 10.1128/JCM.43.2.948-950.2005

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

1.  The absorption of -globulins to Mycoplasma gallisepticum and the possible role in non-specific serological reactions.

Authors:  J M Bradbury; F T Jordan
Journal:  Vet Rec       Date:  1971-09-11       Impact factor: 2.695

2.  Non-specific agglutination of Mycoplasma gallisepticum.

Authors:  J M Bradbury; F T Jordan
Journal:  Vet Rec       Date:  1973-06-02       Impact factor: 2.695

3.  A medium for the isolation of avian mycoplasmas.

Authors:  M L Frey; R P Hanson; D P Andrson
Journal:  Am J Vet Res       Date:  1968-11       Impact factor: 1.156

4.  Cloning of Mycoplasma synoviae genes encoding specific antigens and their use as species-specific DNA probes.

Authors:  B Ben Abdelmoumen; R S Roy; R Brousseau
Journal:  J Vet Diagn Invest       Date:  1999-03       Impact factor: 1.279

5.  Molecular cloning of a member of the gene family that encodes pMGA, a hemagglutinin of Mycoplasma gallisepticum.

Authors:  P F Markham; M D Glew; K G Whithear; I D Walker
Journal:  Infect Immun       Date:  1993-03       Impact factor: 3.441

6.  A novel mechanism for control of antigenic variation in the haemagglutinin gene family of mycoplasma synoviae.

Authors:  A H Noormohammadi; P F Markham; A Kanci; K G Whithear; G F Browning
Journal:  Mol Microbiol       Date:  2000-02       Impact factor: 3.501

7.  Detection of the turkey pathogens Mycoplasma meleagridis and M. iowae by amplification of genes coding for rRNA.

Authors:  J S Boyle; R T Good; C J Morrow
Journal:  J Clin Microbiol       Date:  1995-05       Impact factor: 5.948

8.  Detection of Mycoplasma gallisepticum, M. synoviae, and M. iowae by multi-species polymerase chain reaction and restriction fragment length polymorphism.

Authors:  M Garcia; M W Jackwood; S Levisohn; S H Kleven
Journal:  Avian Dis       Date:  1995 Jul-Sep       Impact factor: 1.577

9.  An enzyme-linked immunosorbent assay for detection of avian mycoplasmas in culture.

Authors:  B B Abdelmoumen; R S Roy
Journal:  Avian Dis       Date:  1995 Jan-Mar       Impact factor: 1.577

10.  Antigenic relatedness between seven avian mycoplasma species as revealed by western blot analysis.

Authors:  B Ben Abdelmoumen; R S Roy
Journal:  Avian Dis       Date:  1995 Apr-Jun       Impact factor: 1.577
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  7 in total

1.  Rapid serodiagnosis with the use of surface plasmon resonance imaging for the detection of antibodies against major surface protein A of Mycoplasma synoviae in chickens.

Authors:  Kiseok Oh; Semi Lee; Jayoung Seo; Dongwoo Lee; Taejung Kim
Journal:  Can J Vet Res       Date:  2010-01       Impact factor: 1.310

2.  Detection and differentiation of avian mycoplasmas by surface-enhanced Raman spectroscopy based on a silver nanorod array.

Authors:  Suzanne L Hennigan; Jeremy D Driskell; Naola Ferguson-Noel; Richard A Dluhy; Yiping Zhao; Ralph A Tripp; Duncan C Krause
Journal:  Appl Environ Microbiol       Date:  2011-12-30       Impact factor: 4.792

3.  A duplex real-time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae.

Authors:  Li Huang; Zhixun Xie; Liji Xie; Xianwen Deng; Zhiqin Xie; Sisi Luo; Jiaoling Huang; Tingting Zeng; Jiaxun Feng
Journal:  Virol J       Date:  2015-02-12       Impact factor: 4.099

4.  Development of a PCR assay to detect mycoplasma contamination in cord blood hematopoietic stem cells.

Authors:  Reza Tabatabaei-Qomi; Mohsen Sheykh-Hasan; Hoda Fazaely; Naser Kalhor; Mahdieh Ghiasi
Journal:  Iran J Microbiol       Date:  2014-08

5.  Molecular identification of Mycoplasma synoviae from seroprevalent commercial breeder farms at Chittagong district, Bangladesh.

Authors:  Md Inkeyas Uddin; Md Harisul Abid; Md Shafiqul Islam; Tofazzal Md Rakib; Ashim Baran Sen; Shah Mohammed Ziqrul Haq Chowdhury; Md Nurul Anwar; Kazi Md Kamaruddin
Journal:  Vet World       Date:  2016-10-10

6.  Genetic variability of the P120' surface protein gene of Mycoplasma hominis isolates recovered from Tunisian patients with uro-genital and infertility disorders.

Authors:  Boutheina Ben Abdelmoumen Mardassi; Hajer Ayari; Awatef Béjaoui-Khiari; Béhija Mlik; Imed Moalla; Faten Amouna
Journal:  BMC Infect Dis       Date:  2007-12-05       Impact factor: 3.090

7.  Isolation and Identification of Mycoplasma synoviae From Suspected Ostriches by Polymerase Chain Reaction, in Kerman Province, Iran.

Authors:  Hamid Tebyanian; Seyed Hanif Mirhosseiny; Babak Kheirkhah; Mehdi Hassanshahian; Hamze Farhadian
Journal:  Jundishapur J Microbiol       Date:  2014-09-01       Impact factor: 0.747
  7 in total

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