Literature DB >> 7537047

Molecular cloning and expression in Escherichia coli of bleomycin-resistance gene from a methicillin-resistant Staphylococcus aureus and its association with IS431 mec.

M Z Bhuiyan1, K Ueda, Y Inouye, M Sugiyama.   

Abstract

A gene that confers bleomycin resistance was cloned from the chromosomal DNA of methicillin-resistant Staphylococcus aureus (MRSA) B-26 into the plasmid pUC18. It is of chromosomal origin rather than plasmid and exists in the chromosome making a cluster with the kanamycin-resistance gene. We found that the nucleotide sequence of the bleomycin-resistance gene from the chromosome of MRSA B-26 is identical to that from a staphylococcal plasmid, pUB110. The partial sequence of IS431mec was also found upstream from the DNA fragment containing the bleomycin- and kanamycin-resistance genes.

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Year:  1995        PMID： 7537047     DOI： 10.1007/bf00170624

Source DB:  PubMed          Journal:  Appl Microbiol Biotechnol        ISSN： 0175-7598            Impact factor:   4.813


  20 in total

1.  An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein.

Authors:  C V Maina; P D Riggs; A G Grandea; B E Slatko; L S Moran; J A Tagliamonte; L A McReynolds; C D Guan
Journal:  Gene       Date:  1988-12-30       Impact factor: 3.688

2.  IS431, a staphylococcal insertion sequence-like element related to IS26 from Proteus vulgaris.

Authors:  L Barberis-Maino; B Berger-Bächi; H Weber; W D Beck; F H Kayser
Journal:  Gene       Date:  1987       Impact factor: 3.688

3.  Restriction maps of the regions coding for methicillin and tobramycin resistances on chromosomal DNA in methicillin-resistant staphylococci.

Authors:  K Ubukata; R Nonoguchi; M Matsuhashi; M D Song; M Konno
Journal:  Antimicrob Agents Chemother       Date:  1989-09       Impact factor: 5.191

4.  Completion of the nucleotide sequence of the central region of Tn5 confirms the presence of three resistance genes.

Authors:  P Mazodier; P Cossart; E Giraud; F Gasser
Journal:  Nucleic Acids Res       Date:  1985-01-11       Impact factor: 16.971

Review 5.  Transposable elements in prokaryotes.

Authors:  N Kleckner
Journal:  Annu Rev Genet       Date:  1981       Impact factor: 16.830

6.  Restriction maps of plasmids pUB110 and pBD9.

Authors:  A Jalanko; I Palva
Journal:  Gene       Date:  1981-09       Impact factor: 3.688

7.  The transposon Tn5 carries a bleomycin-resistance determinant.

Authors:  O Genilloud; M C Garrido; F Moreno
Journal:  Gene       Date:  1984-12       Impact factor: 3.688

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  Characterisation by molecular cloning of two genes from Streptomyces verticillus encoding resistance to bleomycin.

Authors:  M Sugiyama; C J Thompson; T Kumagai; K Suzuki; R Deblaere; R Villarroel; J Davies
Journal:  Gene       Date:  1994-12-30       Impact factor: 3.688

10.  Transposition of R factor genes to bacteriophage lambda.

Authors:  D E Berg; J Davies; B Allet; J D Rochaix
Journal:  Proc Natl Acad Sci U S A       Date:  1975-09       Impact factor: 11.205
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  1 in total

Review 1.  Structural biological study of self-resistance determinants in antibiotic-producing actinomycetes.

Authors:  Masanori Sugiyama
Journal:  J Antibiot (Tokyo)       Date:  2015-04-15       Impact factor: 2.649
  1 in total

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