Literature DB >> 3889831

Completion of the nucleotide sequence of the central region of Tn5 confirms the presence of three resistance genes.

P Mazodier, P Cossart, E Giraud, F Gasser.   

Abstract

The DNA sequence of the region located downstream from the kanamycin resistance gene of Tn5 up to the right inverted repeat IS50R has been determined. This completes the determination of the sequence of Tn5 which is 5818 bp long. The 2.7 Kb central region contains three resistance genes: the kanamycin-neomycin resistance gene, a gene coding for resistance to CL990 an antimitotic-antibiotic compound of the bleomycin family and a third gene that confers streptomycin resistance in some bacterial species but is cryptic in E. coli. A Tn5* mutant able to express streptomycin resistance in E. coli was isolated. With this mutant, it was demonstrated that in E. coli the expression of the three resistance genes is coordinated in a single operon.

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Year:  1985        PMID： 3889831      PMCID： PMC340984          DOI： 10.1093/nar/13.1.195

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  27 in total

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Authors:  R A Jorgensen; S J Rothstein; W S Reznikoff
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Journal:  Annu Rev Genet       Date:  1979       Impact factor: 16.830

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Journal:  Annu Rev Microbiol       Date:  1978       Impact factor: 15.500

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Journal:  Biochim Biophys Acta       Date:  1975-04-02

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Journal:  FEBS Lett       Date:  1978-03-01       Impact factor: 4.124

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Journal:  Eur J Biochem       Date:  1971-10-14

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Authors:  G F De Vos; T M Finan; E R Signer; G C Walker
Journal:  J Bacteriol       Date:  1984-07       Impact factor: 3.490

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Authors:  D E Berg; J Davies; B Allet; J D Rochaix
Journal:  Proc Natl Acad Sci U S A       Date:  1975-09       Impact factor: 11.205
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  67 in total

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Journal:  Appl Microbiol Biotechnol       Date:  1992-03       Impact factor: 4.813

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Authors:  C Li; S Clarke
Journal:  Proc Natl Acad Sci U S A       Date:  1992-10-15       Impact factor: 11.205

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Journal:  Proc Natl Acad Sci U S A       Date:  1991-07-15       Impact factor: 11.205

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Journal:  Plant Mol Biol       Date:  1987-03       Impact factor: 4.076

6.  A temperature-dependent pBR322 copy number mutant resulting from a Tn5 position effect.

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7.  Transformation of rapid cycling cabbage (Brassica oleracea var. capitata) with Agrobacterium rhizogenes.

Authors:  P Berthomieu; L Jouanin
Journal:  Plant Cell Rep       Date:  1992-07       Impact factor: 4.570

8.  Stable expression of antibiotic resistance genes using a promoter fragment of the U1 snRNA gene.

Authors:  F A Asselbergs; R Pronk
Journal:  Mol Biol Rep       Date:  1993-02       Impact factor: 2.316

9.  Construction and characterization of a proU-gfp transcriptional fusion that measures water availability in a microbial habitat.

Authors:  Catherine A Axtell; Gwyn A Beattie
Journal:  Appl Environ Microbiol       Date:  2002-09       Impact factor: 4.792

10.  Mutations in two new loci that impair both extracellular protein production and development in Myxococcus xanthus.

Authors:  F Petit; M Merah; C Monnier; J F Guespin-Michel
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490
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