Literature DB >> 7534475

High-affinity RNA ligands to Escherichia coli ribosomes and ribosomal protein S1: comparison of natural and unnatural binding sites.

S Ringquist1, T Jones, E E Snyder, T Gibson, I Boni, L Gold.   

Abstract

High-affinity RNA ligands were generated against intact 30S ribosomes, S1-depleted 30S ribosomes, and purified ribosomal protein S1. Sequence analysis indicated two classes of ligand: unstructured RNAs containing a Shine-Dalgarno sequence and structured RNAs containing a pseudoknot. The Shine-Dalgarno-containing ligands were generated against S1-depleted 30S ribosomes but, surprisingly, not against intact 30S ribosomes or ribosomal protein S1. In contrast, pseudoknot-containing ligands were generated against intact ribosomes as well as purified S1 protein. The two classes of ligand exhibited specificity for their respective targets, as well as conserved sequence and secondary structure reminiscent of naturally occurring, cis-acting mRNA elements.

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Year:  1995        PMID： 7534475     DOI： 10.1021/bi00011a019

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  37 in total

1.  Negative in vitro selection identifies the rRNA recognition motif for ErmE methyltransferase.

Authors:  A K Nielsen; S Douthwaite; B Vester
Journal:  RNA       Date:  1999-08       Impact factor: 4.942

2.  Enhancement of translation by the downstream box does not involve base pairing of mRNA with the penultimate stem sequence of 16S rRNA.

Authors:  M O'Connor; T Asai; C L Squires; A E Dahlberg
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-03       Impact factor: 11.205

3.  Protein factors associated with the SsrA.SmpB tagging and ribosome rescue complex.

Authors:  A W Karzai; R T Sauer
Journal:  Proc Natl Acad Sci U S A       Date:  2001-02-27       Impact factor: 11.205

4.  Binding and cross-linking of tmRNA to ribosomal protein S1, on and off the Escherichia coli ribosome.

Authors:  I K Wower; C W Zwieb; S A Guven; J Wower
Journal:  EMBO J       Date:  2000-12-01       Impact factor: 11.598

5.  Visualization of protein S1 within the 30S ribosomal subunit and its interaction with messenger RNA.

Authors:  J Sengupta; R K Agrawal; J Frank
Journal:  Proc Natl Acad Sci U S A       Date:  2001-10-02       Impact factor: 11.205

6.  RNA-binding characteristics of the chloroplast S1-like ribosomal protein CS1.

Authors:  A Shteiman-Kotler; G Schuster
Journal:  Nucleic Acids Res       Date:  2000-09-01       Impact factor: 16.971

7.  Protein S1 counteracts the inhibitory effect of the extended Shine-Dalgarno sequence on translation.

Authors:  Anastassia V Komarova; Ludmila S Tchufistova; Elena V Supina; Irina V Boni
Journal:  RNA       Date:  2002-09       Impact factor: 4.942

Review 8.  Evolutionary conservation of reactions in translation.

Authors:  M Clelia Ganoza; Michael C Kiel; Hiroyuki Aoki
Journal:  Microbiol Mol Biol Rev       Date:  2002-09       Impact factor: 11.056

9.  Ribosomal protein S1 binds mRNA and tmRNA similarly but plays distinct roles in translation of these molecules.

Authors:  Kathleen E McGinness; Robert T Sauer
Journal:  Proc Natl Acad Sci U S A       Date:  2004-08-31       Impact factor: 11.205

10.  Robust translation of the nucleoid protein Fis requires a remote upstream AU element and is enhanced by RNA secondary structure.

Authors:  Maryam Nafissi; Jeannette Chau; Jimin Xu; Reid C Johnson
Journal:  J Bacteriol       Date:  2012-03-02       Impact factor: 3.490
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